Restriction map for
pFN21AE3464
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# NO CUT ENZYMES ( 58 enzymes) #
##################################
AclI AflII AgeI Aor51HI ApaI
AscI AseI AsnI AvrII BbvCI
BfrI BlnI BsiWI Bsp1407I BspDI
BsrGI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI DraI DrdI Eco47III
EcoT22I FseI HindIII HpaI MfeI
MluI MunI NcoI NdeI NheI
NruI NsiI NspV PacI PinAI
PmeI PshBI Psp1406I PvuI RsrII
SalI SbfI ScaI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SwaI XbaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Acc65I 562 GGTACC G^GTACC
AccI 1675 GTAGAC GT^[AC][GT]AC
AflIII 3617 ACATGT A^C[GA][CT]GT
Alw44I 981 GTGCAC G^TGCAC
ApaLI 981 GTGCAC G^TGCAC
Asp718I 562 GGTACC G^GTACC
AspI 2049 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3770 TGCGCA TGC^GCA
BamHI 1878 GGATCC G^GATCC
BbrPI 3217 CACGTG CAC^GTG
BciVI 485 GTATCC GTATCC(6/5)
BglII 1532 AGATCT A^GATCT
BlpI 1135 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1135 GCTGAGC GC^T[ACGT]AGC
BsaBI 408 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 2661 CGTCTC CGTCTC(1/5)
BsmI 2122 GAATGC GAATGC(1/-1)
BspLU11I 3617 ACATGT A^CATGT
BsrBI 3163 GAGCGG CCGCTC(-3/-3)
BssSI 2331 CACGAG CACGAG(-5/-1)
BstEII 1788 GGTCACC G^GT[ACGT]ACC
BstPI 1788 GGTCACC G^GT[ACGT]ACC
BstXI 749 CCAAGTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1135 GCTGAGC GC^T[ACGT]AGC
EcoO65I 1788 GGTCACC G^GT[ACGT]ACC
EcoRI 963 GAATTC G^AATTC
EcoRV 115 GATATC GAT^ATC
FspI 3770 TGCGCA TGC^GCA
KpnI 562 GGTACC GGTAC^C
KspI 74 CCGCGG CCGC^GG
MamI 408 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NotI 28 GCGGCCGC GC^GGCCGC
PflFI 2049 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3217 CACGTG CAC^GTG
PmlI 3217 CACGTG CAC^GTG
PvuII 1133 CAGCTG CAG^CTG
SacII 74 CCGCGG CCGC^GG
SmaI 419 CCCGGG CCC^GGG
SspI 2821 AATATT AAT^ATT
Tth111I 2049 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 746 CCACCAAGTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 419 CCCGGG C^CCGGG
XmaI 419 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 35 enzymes) #
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AatII 1053 GACGTC GACGT^C
2809 GACGTC
Asp700I 1353 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3198 GAAGGGCTTC
BalI 2 TGGCCA TGG^CCA
464 TGGCCA
BbeI 70 GGCGCC GGCGC^C
1430 GGCGCC
BclI 248 TGATCA T^GATCA
1100 TGATCA
BglI 1551 GCCGTACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2913 GCCAGATGGGC
BsiEI 29 CGGCCG CG[GA][CT]^CG
2114 CGGCCG
BspHI 245 TCATGA T^CATGA
3194 TCATGA
BsrDI 1449 CATTGC GCAATG(2/0)
3083 GCAATG
BsrFI 14 GCCGGC [GA]^CCGG[CT]
2116 GCCGGC
BstAPI 983 GCACCCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3385 GCAGAGATTGC
Cfr10I 14 GCCGGC [GA]^CCGG[CT]
2116 GCCGGC
EagI 29 CGGCCG C^GGCCG
2114 CGGCCG
EclXI 29 CGGCCG C^GGCCG
2114 CGGCCG
Eco52I 29 CGGCCG C^GGCCG
2114 CGGCCG
FbaI 248 TGATCA T^GATCA
1100 TGATCA
HgaI 2535 GACGC GACGC(5/10)
2660 GCGTC
HincII 3139 GTCAAC GT[CT]^[GA]AC
3823 GTCAAC
HindII 3139 GTCAAC GT[CT]^[GA]AC
3823 GTCAAC
KasI 70 GGCGCC G^GCGCC
1430 GGCGCC
MluNI 2 TGGCCA TGG^CCA
464 TGGCCA
MscI 2 TGGCCA TGG^CCA
464 TGGCCA
MseI 347 TTAA T^TAA
1499 TTAA
NaeI 14 GCCGGC GCC^GGC
2116 GCCGGC
NarI 70 GGCGCC GG^CGCC
1430 GGCGCC
NgoMIV 14 GCCGGC G^CCGGC
2116 GCCGGC
NspI 3617 ACATGT [GA]CATG^[CT]
3773 GCATGT
PstI 2428 CTGCAG CTGCA^G
2694 CTGCAG
RcaI 245 TCATGA T^CATGA
3194 TCATGA
SacI 451 GAGCTC GAGCT^C
1066 GAGCTC
SapI 3297 GAAGAGC GCTCTTC(1/4)
267 GCTCTTC
SexAI 1964 ACCTGGT A^CC[AT]GGT
2050 ACCTGGT
SfoI 70 GGCGCC GGC^GCC
1430 GGCGCC
Tru9I 347 TTAA T^TAA
1499 TTAA
XmnI 1353 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3198 GAAGGGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AccIII AciI AcsI AcyI
AfaI AhdI AluI AlwI AlwNI
AocI ApoI AspEI AspHI AvaI
AvaII BanI BanII BbsI BbvI
BcgI BcnI BfaI BmyI BpmI
BpuAI BsaAI BsaHI BsaI BsaJI
BsaWI BseAI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspEI BspMI BsrI BssKI
BstF5I BstNI BstUI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI Eam1105I
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MroI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PaeR7I PflMI
PleI PpuMI PshAI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoI XhoII