Restriction map for
pFN21AE3403
##################################
# NO CUT ENZYMES ( 196 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AfaI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI AscI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BamHI BanII
BbrPI BbvI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
BmyI BpmI Bpu1102I BsaAI BsaBI
BsaI BsaJI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsmI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DpnII DraI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRV EcoT14I
EcoT22I FauI FbaI FseI FspI
HapII HincII HindII HindIII HinfI
HpaI HpaII HphI KpnI Ksp632I
KspI MaeII MamI MboI MfeI
MflI MluI MluNI MroI MscI
MspA1I MspI MunI MvnI NaeI
NciI NcoI NdeI NdeII NgoMIV
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PstI PvuI PvuII
RcaI RsaI RsrII SacI SacII
SalI SapI Sau3AI SbfI ScaI
SexAI SfcI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TfiI TseI Tth111I Van91I
XcmI XhoI XhoII XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AciI 484 CCGC CCGC(-3/-1)
AcyI 443 GGCGCC G[GA]^CG[CT]C
AseI 331 ATTAAT AT^TAAT
AsnI 331 ATTAAT AT^TAAT
BanI 443 GGCGCC G^G[CT][GA]CC
BbeI 443 GGCGCC GGCGC^C
BbvCI 478 CCTCAGC CCTCAGC(-5/-2)
BcgI 432 GCAAGAGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaHI 443 GGCGCC G[GA]^CG[CT]C
BsmFI 100 GTCCC GGGAC(10/14)
BssKI 4 CCAGG ^CC[ACGT]GG
BstF5I 218 GGATG GGATG(2/0)
BstNI 4 CCAGG CC^[AT]GG
Cac8I 390 GCTAGC GC[ACGT]^[ACGT]GC
CfoI 444 GCGC GCG^C
EcoRI 405 GAATTC G^AATTC
EcoRII 4 CCAGG ^CC[AT]GG
Fnu4HI 483 GCCGC GC^[ACGT]GC
FokI 218 GGATG GGATG(9/13)
HaeII 443 GGCGCC [GA]GCGC^[CT]
HgaI 19 GCGTC GACGC(5/10)
HhaI 444 GCGC GCG^C
Hin1I 443 GGCGCC G[GA]^CG[CT]C
HinP1I 444 GCGC G^CGC
ItaI 483 GCCGC GC^[ACGT]GC
KasI 443 GGCGCC G^GCGCC
MslI 381 CATATCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 4 CCAGG CC^[AT]GG
MwoI 161 GCACAGTTAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NarI 443 GGCGCC GG^CGCC
NheI 390 GCTAGC G^CTAGC
NlaIII 195 CATG CATG^
PshBI 331 ATTAAT AT^TAAT
ScrFI 4 CCAGG CC^[ACGT]GG
SfaNI 416 GCATC GCATC(5/9)
SfoI 443 GGCGCC GGC^GCC
TaqI 131 TCGA T^CGA
Tsp45I 33 GTCAC ^GT[GC]AC
TthHB8I 131 TCGA T^CGA
XbaI 177 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AcsI 76 AAATTT [GA]^AATT[CT]
405 GAATTC
AluI 169 AGCT AG^CT
437 AGCT
ApoI 76 AAATTT [GA]^AATT[CT]
405 GAATTC
AvaII 283 GGACC G^G[AT]CC
369 GGACC
BbsI 59 GAAGAC GAAGAC(2/6)
190 GAAGAC
BpuAI 59 GAAGAC GAAGAC(2/6)
190 GAAGAC
BsiYI 114 CCGACTGTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
484 CCGCCCGAAGG
BslI 114 CCGACTGTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
484 CCGCCCGAAGG
DdeI 46 CTGAG C^T[ACGT]AG
479 CTCAG
HaeIII 139 GGCC GG^CC
230 GGCC
MseI 269 TTAA T^TAA
332 TTAA
NlaIV 369 GGACCC GG[ACGT]^[ACGT]CC
443 GGCGCC
Tru9I 269 TTAA T^TAA
332 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 9 enzymes) #
#########################################
BfaI Cfr13I MaeI MaeIII MboII
MnlI Sau96I Tsp509I TspRI