Restriction map for
pFN21AE3401
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# NO CUT ENZYMES ( 197 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI AluI Alw44I AlwNI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BciVI BclI BcnI BfrI BglI
BglII BlnI BlpI BmyI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaJI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssKI BssSI Bst1107I BstAPI
BstBI BstEII BstNI BstPI BstUI
BstZ17I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRII EcoRV EcoT14I EcoT22I FauI
FbaI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HpaII HphI
KasI KpnI Ksp632I KspI MaeII
MamI MboII MfeI MluI MluNI
MroI MscI MspA1I MspI MunI
MvaI MvnI MwoI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI ScrFI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
StuI StyI SwaI TfiI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AciI 146 GCGG CCGC(-3/-1)
AcsI 221 GAATTT [GA]^AATT[CT]
AfaI 405 GTAC GT^AC
AocI 199 CCTAAGG CC^T[ACGT]AGG
ApoI 221 GAATTT [GA]^AATT[CT]
AvaI 419 CTCGGG C^[CT]CG[GA]G
BbvI 251 GCAGC GCAGC(8/12)
BcgI 254 GCATTTGTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 361 CTAG C^TAG
BpmI 56 CTCCAG CTGGAG(16/14)
BsiYI 136 CCATATCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 136 CCATATCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 260 GTCTC GTCTC(1/5)
BsmFI 120 GGGAC GGGAC(10/14)
BsoBI 419 CTCGGG C^[CT]CG[GA]G
BspMI 73 GCAGGT ACCTGC(4/8)
BsrI 79 CCAGT ACTGG(1/-1)
BstXI 110 CCACAATTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 380 GGATCT [GA]^GATC[CT]
Bsu36I 199 CCTAAGG CC^T[ACGT]AGG
DraII 120 GGGACCT [GA]G^G[ACGT]CC[CT]
Eco81I 199 CCTAAGG CC^T[ACGT]AGG
EcoO109I 120 GGGACCT [GA]G^G[ACGT]CC[CT]
Fnu4HI 251 GCAGC GC^[ACGT]GC
HaeIII 45 GGCC GG^CC
HinfI 429 GACTC G^A[ACGT]TC
ItaI 251 GCAGC GC^[ACGT]GC
MaeI 361 CTAG C^TAG
MaeIII 50 GTGAC ^GT[ACGT]AC
MflI 380 GGATCT [GA]^GATC[CT]
MnlI 55 CCTC CCTC(7/6)
NlaIII 444 CATG CATG^
NlaIV 120 GGGACC GG[ACGT]^[ACGT]CC
PleI 429 GACTC GAGTC(4/5)
PpuMI 120 GGGACCT [GA]G^G[AT]CC[CT]
PstI 71 CTGCAG CTGCA^G
RsaI 405 GTAC GT^AC
SspI 235 AATATT AAT^ATT
TaqI 263 TCGA T^CGA
TseI 251 GCAGC G^C[AT]GC
Tsp45I 50 GTGAC ^GT[GC]AC
TthHB8I 263 TCGA T^CGA
XhoII 380 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 10 enzymes) #
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AlwI 340 GATCC GGATC(4/5)
380 GGATC
AvaII 76 GGTCC G^G[AT]CC
121 GGACC
BstF5I 229 CATCC GGATG(2/0)
87 GGATG
Cac8I 105 GCAAGC GC[ACGT]^[ACGT]GC
288 GCCTGC
FokI 229 CATCC GGATG(9/13)
87 GGATG
MseI 239 TTAA T^TAA
295 TTAA
MslI 111 CACAATTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
329 CACTGCTATG
SfcI 71 CTGCAG C^T[GA][CT]AG
176 CTACAG
Tru9I 239 TTAA T^TAA
295 TTAA
TspRI 280 CAGTG CA[GC]TG[ACGT][ACGT]^
329 CACTG
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# 3 OR MORE CUT ENZYMES ( 8 enzymes) #
#########################################
Cfr13I DdeI DpnII MboI NdeII
Sau3AI Sau96I Tsp509I