Restriction map for
pFN21AB7746
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# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AccIII AcyI AgeI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp718I AspI AvaI AviII
AvrII BamHI BbeI BbrPI BbsI
BcgI BcnI BfaI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaHI
BsaWI BseAI BsgI BsiEI BsiWI
BsmI BsoBI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrGI BssHII BssSI
BstBI BstEII BstPI Bsu36I CelII
ClaI CpoI DraI DraIII DrdI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoT22I FseI
FspI HaeII HgaI Hin1I HindIII
KasI KpnI KspI MaeI MfeI
MluI MroI MunI NarI NciI
NdeI NheI NotI NruI NsiI
NspV PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PshAI PvuI
RsrII SacI SacII SalI SapI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI Tth111I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccI 130 GTATAC GT^[AC][GT]AC
AccII 29 CGCG CG^CG
AclI 357 AACGTT AA^CGTT
AcsI 1260 AAATTC [GA]^AATT[CT]
AflIII 1076 ACACGT A^C[GA][CT]GT
AhdI 616 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 820 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 1260 AAATTC [GA]^AATT[CT]
AseI 880 ATTAAT AT^TAAT
AsnI 880 ATTAAT AT^TAAT
AspEI 616 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1066 GAGCAC G[AT]GC[AT]^C
BalI 533 TGGCCA TGG^CCA
BanI 1096 GGCACC G^G[CT][GA]CC
BanII 792 GAGCCC G[GA]GC[CT]^C
BbvCI 15 GCTGAGG CCTCAGC(-5/-2)
BciVI 598 GGATAC GTATCC(6/5)
BclI 1106 TGATCA T^GATCA
BsaBI 676 GATATCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 719 CTCCTC GAGGAG(10/8)
BsiHKAI 1066 GAGCAC G[AT]GC[AT]^C
BspHI 701 TCATGA T^CATGA
BspMI 511 GCAGGT ACCTGC(4/8)
BsrDI 234 GCAATG GCAATG(2/0)
Bst1107I 130 GTATAC GTA^TAC
BstAPI 487 GCAAAACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 29 CGCG CG^CG
BstYI 1382 AGATCC [GA]^GATC[CT]
BstZ17I 130 GTATAC GTA^TAC
CfoI 30 GCGC GCG^C
Eam1105I 616 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 1106 TGATCA T^GATCA
HhaI 30 GCGC GCG^C
HinP1I 30 GCGC G^CGC
HpaI 360 GTTAAC GTT^AAC
MaeIII 239 GTGAC ^GT[ACGT]AC
MamI 676 GATATCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1382 AGATCC [GA]^GATC[CT]
MluNI 533 TGGCCA TGG^CCA
MscI 533 TGGCCA TGG^CCA
MvnI 29 CGCG CG^CG
NaeI 32 GCCGGC GCC^GGC
NgoMIV 32 GCCGGC G^CCGGC
NspI 483 ACATGC [GA]CATG^[CT]
PacI 877 TTAATTAA TTAAT^TAA
PflMI 1282 CCACAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 880 ATTAAT AT^TAAT
Psp1406I 357 AACGTT AA^CGTT
PstI 826 CTGCAG CTGCA^G
RcaI 701 TCATGA T^CATGA
ScaI 308 AGTACT AGT^ACT
SexAI 1245 ACCTGGT A^CC[AT]GGT
SfaNI 1423 GATGC GCATC(5/9)
SfcI 826 CTGCAG C^T[GA][CT]AG
Tsp45I 239 GTGAC ^GT[GC]AC
Van91I 1282 CCACAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1382 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AflII 400 CTTAAG C^TTAAG
1306 CTTAAG
AlwI 1383 GATCC GGATC(4/5)
296 GGATC
Asp700I 412 GAATACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
580 GAAGTGATTC
BfrI 400 CTTAAG C^TTAAG
1306 CTTAAG
BglI 27 GCCGCGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1426 GCCATAGAGGC
BpmI 199 CTCCAG CTGGAG(16/14)
575 CTCCAG
BsaI 151 GAGACC GGTCTC(1/5)
1156 GAGACC
BsmBI 4 GAGACG CGTCTC(1/5)
1443 GAGACG
BsmFI 405 GGGAC GGGAC(10/14)
624 GTCCC
BsrFI 32 GCCGGC [GA]^CCGG[CT]
154 ACCGGC
BsrI 1092 ACTGG ACTGG(1/-1)
1398 CCAGT
BstXI 641 CCATAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
739 CCAGAGCTATGG
Cfr10I 32 GCCGGC [GA]^CCGG[CT]
154 ACCGGC
DraII 115 GGGTCCT [GA]G^G[ACGT]CC[CT]
1325 AGGACCT
EaeI 156 CGGCCA [CT]^GGCC[GA]
533 TGGCCA
EcoO109I 115 GGGTCCT [GA]G^G[ACGT]CC[CT]
1325 AGGACCT
FauI 24 CCCGC CCCGC(4/6)
927 GCGGG
HincII 360 GTTAAC GT[CT]^[GA]AC
515 GTCAAC
HindII 360 GTTAAC GT[CT]^[GA]AC
515 GTCAAC
HphI 1003 GGTGA GGTGA(8/7)
1104 GGTGA
MaeII 358 ACGT A^CGT
1078 ACGT
PpuMI 115 GGGTCCT [GA]G^G[AT]CC[CT]
1325 AGGACCT
PvuII 13 CAGCTG CAG^CTG
37 CAGCTG
SmlI 400 CTTAAG C^T[CT][GA]AG
1306 CTTAAG
TaqI 342 TCGA T^CGA
993 TCGA
TthHB8I 342 TCGA T^CGA
993 TCGA
XmnI 412 GAATACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
580 GAAGTGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AfaI AluI AvaII BbvI
BmyI BsaJI BsiYI BslI BsmAI
Bsp1286I BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DsaI EarI
EcoRII EcoRV EcoT14I Fnu4HI FokI
HaeIII HapII HinfI HpaII ItaI
Ksp632I MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MwoI
NcoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SspI StyI
TfiI Tru9I TseI Tsp509I TspRI