Restriction map for
pFN21AB7645
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# NO CUT ENZYMES ( 136 enzymes) #
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AatII AccI AclI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
ApaI ApaLI AscI AseI AsnI
AspEI AspHI AspI AviII AvrII
BalI BbrPI BbsI BbvCI BcgI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsaI
BseRI BsiEI BsiHKAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DrdI EaeI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HgaI HincII HindII
HindIII HpaI KspI MaeIII MamI
MfeI MluI MluNI MscI MseI
MslI MunI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI SspBI
SspI StuI StyI SwaI TfiI
Tru9I Tsp45I Tth111I Van91I XbaI
XhoI
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# ONE CUT ENZYMES ( 47 enzymes) #
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Acc65I 845 GGTACC G^GTACC
AccIII 455 TCCGGA T^CCGGA
AcsI 422 AAATTC [GA]^AATT[CT]
Aor51HI 673 AGCGCT AGC^GCT
ApoI 422 AAATTC [GA]^AATT[CT]
Asp700I 368 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 845 GGTACC G^GTACC
BamHI 862 GGATCC G^GATCC
BfaI 22 CTAG C^TAG
BsaAI 63 CACGTA [CT]AC^GT[GA]
BseAI 455 TCCGGA T^CCGGA
BsgI 898 CTGCAC GTGCAG(16/14)
BsmAI 703 GTCTC GTCTC(1/5)
BspEI 455 TCCGGA T^CCGGA
BspMI 875 ACCTGC ACCTGC(4/8)
BsrBI 90 GAGCGG CCGCTC(-3/-3)
BstF5I 743 GGATG GGATG(2/0)
DraII 407 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 683 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 884 CCACGG C^C[GA][CT]GG
EarI 497 CTCTTC CTCTTC(1/4)
Eco47III 673 AGCGCT AGC^GCT
EcoO109I 407 AGGACCT [GA]G^G[ACGT]CC[CT]
FokI 743 GGATG GGATG(9/13)
KpnI 845 GGTACC GGTAC^C
Ksp632I 497 CTCTTC CTCTTC(1/4)
MaeI 22 CTAG C^TAG
MroI 455 TCCGGA T^CCGGA
NlaIII 258 CATG CATG^
PpuMI 407 AGGACCT [GA]G^G[AT]CC[CT]
PstI 877 CTGCAG CTGCA^G
PvuII 487 CAGCTG CAG^CTG
SapI 496 GCTCTTC GCTCTTC(1/4)
SbfI 876 CCTGCAGG CCTGCA^GG
SfaNI 924 GCATC GCATC(5/9)
SfcI 877 CTGCAG C^T[GA][CT]AG
SmaI 108 CCCGGG CCC^GGG
SmlI 412 CTTGAG C^T[CT][GA]AG
Sse8387I 876 CCTGCAGG CCTGCA^GG
TaqI 40 TCGA T^CGA
Tsp509I 423 AATT ^AATT
TspRI 768 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 40 TCGA T^CGA
XcmI 479 CCAGTTTCCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 108 CCCGGG C^CCGGG
XmaI 108 CCCGGG C^CCGGG
XmnI 368 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 11 enzymes) #
###################################
BanII 240 GGGCTC G[GA]GC[CT]^C
795 GGGCTC
BglI 107 GCCCGGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
547 GCCGAGCTGGC
BmyI 240 GGGCTC G[AGT]GC[ACT]^C
795 GGGCTC
BsaWI 168 TCCGGT [AT]^CCGG[AT]
455 TCCGGA
Bsp1286I 240 GGGCTC G[AGT]GC[ACT]^C
795 GGGCTC
BstYI 644 AGATCC [GA]^GATC[CT]
862 GGATCC
MaeII 64 ACGT A^CGT
296 ACGT
MboII 624 GAAGA GAAGA(8/7)
498 TCTTC
MflI 644 AGATCC [GA]^GATC[CT]
862 GGATCC
PshAI 3 GACTGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
579 GACTCAGGTC
XhoII 644 AGATCC [GA]^GATC[CT]
862 GGATCC
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# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AvaI AvaII BanI BbeI
BbvI BcnI BpmI BsaHI BsaJI
BsiYI BslI BsmFI BsoBI BsrFI
BsrI BssKI BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
EcoRII FauI Fnu4HI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI MboI
MnlI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NdeII
NgoMIV NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfoI TseI