Restriction map for
pFN21AE3385
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# NO CUT ENZYMES ( 84 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AcyI AflII AocI Aor51HI ApoI
AscI AseI AsnI Asp700I Asp718I
AviII BbeI BcgI BfrI BsaBI
BsaHI BseAI BsiWI Bsp1407I BspDI
BspEI BspHI BsrBI BsrDI BsrGI
BssSI Bst1107I BstBI BstZ17I Bsu36I
ClaI DraI DrdI Eco47III Eco81I
EcoNI EcoRI EcoRV EcoT22I FseI
FspI Hin1I HindIII HpaI KasI
KpnI KspI MamI MluI MroI
NarI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PmeI
PshBI Psp1406I PvuI RcaI SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SnaBI SpeI Sse8387I
SspBI SwaI XhoI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccII 3299 CGCG CG^CG
AgeI 2233 ACCGGT A^CCGGT
AhdI 1147 GACATGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 1147 GACATGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 713 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 951 CCTAGG C^CTAGG
BamHI 999 GGATCC G^GATCC
BclI 1221 TGATCA T^GATCA
BglI 2026 GCCCAGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1646 AGATCT A^GATCT
BlnI 951 CCTAGG C^CTAGG
BsaI 2137 GAGACC GGTCTC(1/5)
BsaWI 2233 ACCGGT [AT]^CCGG[AT]
BsiEI 2998 CGGCCG CG[GA][CT]^CG
BsmBI 1056 CGTCTC CGTCTC(1/5)
BsmI 793 GAATGC GAATGC(1/-1)
BspMI 918 GCAGGT ACCTGC(4/8)
BssHII 3298 GCGCGC G^CGCGC
BstUI 3299 CGCG CG^CG
BstXI 378 CCAAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CpoI 3065 CGGACCG CG^G[AT]CCG
EagI 2998 CGGCCG C^GGCCG
Eam1105I 1147 GACATGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2998 CGGCCG C^GGCCG
Eco52I 2998 CGGCCG C^GGCCG
FbaI 1221 TGATCA T^GATCA
MvnI 3299 CGCG CG^CG
NaeI 3240 GCCGGC GCC^GGC
NgoMIV 3240 GCCGGC G^CCGGC
PflFI 713 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2233 ACCGGT A^CCGGT
RsrII 3065 CGGACCG CG^G[AT]CCG
SgrAI 3239 CGCCGGCG C[GA]^CCGG[CT]G
SspI 1265 AATATT AAT^ATT
Tth111I 713 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1006 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 25 enzymes) #
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AccI 504 GTCTAC GT^[AC][GT]AC
3460 GTCTAC
Alw44I 2452 GTGCAC G^TGCAC
3166 GTGCAC
ApaLI 2452 GTGCAC G^TGCAC
3166 GTGCAC
BalI 2038 TGGCCA TGG^CCA
2096 TGGCCA
BbvCI 287 CCTCAGC CCTCAGC(-5/-2)
1784 CCTCAGC
BciVI 3469 GGATAC GTATCC(6/5)
3405 GTATCC
BlpI 1993 GCTCAGC GC^T[ACGT]AGC
3046 GCTCAGC
Bpu1102I 1993 GCTCAGC GC^T[ACGT]AGC
3046 GCTCAGC
BstEII 282 GGTCACC G^GT[ACGT]ACC
2481 GGTCACC
BstPI 282 GGTCACC G^GT[ACGT]ACC
2481 GGTCACC
CelII 1993 GCTCAGC GC^T[ACGT]AGC
3046 GCTCAGC
EcoO65I 282 GGTCACC G^GT[ACGT]ACC
2481 GGTCACC
HaeII 1276 AGCGCC [GA]GCGC^[CT]
3439 AGCGCC
HincII 2884 GTCAAC GT[CT]^[GA]AC
3493 GTCAAC
HindII 2884 GTCAAC GT[CT]^[GA]AC
3493 GTCAAC
MfeI 1142 CAATTG C^AATTG
2540 CAATTG
MluNI 2038 TGGCCA TGG^CCA
2096 TGGCCA
MscI 2038 TGGCCA TGG^CCA
2096 TGGCCA
MunI 1142 CAATTG C^AATTG
2540 CAATTG
SacI 1991 GAGCTC GAGCT^C
2964 GAGCTC
SmaI 2519 CCCGGG CCC^GGG
3368 CCCGGG
SmlI 391 CTCAAG C^T[CT][GA]AG
1979 CTCAAG
StuI 1916 AGGCCT AGG^CCT
3095 AGGCCT
XmaCI 2519 CCCGGG C^CCGGG
3368 CCCGGG
XmaI 2519 CCCGGG C^CCGGG
3368 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BanI BanII BbrPI BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspLU11I BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PmaCI PmlI PpuMI
PshAI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SphI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII