Restriction map for
pFN21AB8559
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# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII AccI AclI AcsI AcyI
AflII AgeI AhdI AlwNI AocI
Aor51HI ApaI ApoI AscI AseI
AsnI AspEI AspI AviII AvrII
BamHI BbeI BbvCI BcgI BclI
BfrI BlnI BlpI Bpu1102I BsaHI
BsiEI BsiWI BsmBI BspDI BsrBI
BssSI Bst1107I BstZ17I Bsu36I CelII
ClaI CpoI DraII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO109I EcoRI FauI FbaI FseI
FspI HgaI Hin1I HpaI KasI
KspI MfeI MluI MunI NaeI
NarI NgoMIV NheI NotI NruI
PacI PaeR7I PflFI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI SfiI SfoI SgrAI SmaI
SnaBI SpeI SphI Sse8387I StuI
SwaI Tth111I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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Acc65I 2227 GGTACC G^GTACC
AccIII 116 TCCGGA T^CCGGA
Alw44I 324 GTGCAC G^TGCAC
ApaLI 324 GTGCAC G^TGCAC
Asp700I 2563 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2227 GGTACC G^GTACC
BanII 129 GAGCCC G[GA]GC[CT]^C
BbrPI 2534 CACGTG CAC^GTG
BbsI 1638 GAAGAC GAAGAC(2/6)
BglI 101 GCCATGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 762 AGATCT A^GATCT
BpuAI 1638 GAAGAC GAAGAC(2/6)
BsaI 2957 GAGACC GGTCTC(1/5)
BsaWI 116 TCCGGA [AT]^CCGG[AT]
BseAI 116 TCCGGA T^CCGGA
BspEI 116 TCCGGA T^CCGGA
BsrFI 226 ACCGGC [GA]^CCGG[CT]
BssHII 218 GCGCGC G^CGCGC
BstBI 2303 TTCGAA TT^CGAA
BstYI 762 AGATCT [GA]^GATC[CT]
Cfr10I 226 ACCGGC [GA]^CCGG[CT]
DraI 1140 TTTAAA TTT^AAA
DraIII 327 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1864 CCATGG C^C[GA][CT]GG
EcoNI 694 CCTGTGCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1226 ATGCAT ATGCA^T
HindIII 2071 AAGCTT A^AGCTT
KpnI 2227 GGTACC GGTAC^C
MflI 762 AGATCT [GA]^GATC[CT]
MroI 116 TCCGGA T^CCGGA
NcoI 1864 CCATGG C^CATGG
NdeI 578 CATATG CA^TATG
NsiI 1226 ATGCAT ATGCA^T
NspV 2303 TTCGAA TT^CGAA
PflMI 274 CCAATCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2534 CACGTG CAC^GTG
PmlI 2534 CACGTG CAC^GTG
PstI 1205 CTGCAG CTGCA^G
ScaI 1542 AGTACT AGT^ACT
SexAI 749 ACCTGGT A^CC[AT]GGT
SfuI 2303 TTCGAA TT^CGAA
SmlI 2778 CTCAAG C^T[CT][GA]AG
SspI 2788 AATATT AAT^ATT
Van91I 274 CCAATCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2898 TCTAGA T^CTAGA
XcmI 1506 CCATGCAGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 762 AGATCT [GA]^GATC[CT]
XmnI 2563 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 27 enzymes) #
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AccII 58 CGCG CG^CG
219 CGCG
AlwI 2344 GATCC GGATC(4/5)
1210 GGATC
AspHI 324 GTGCAC G[AT]GC[AT]^C
2463 GTGCTC
AvaI 47 CTCGGG C^[CT]CG[GA]G
133 CCCGAG
BalI 1389 TGGCCA TGG^CCA
2643 TGGCCA
BciVI 2097 GGATAC GTATCC(6/5)
891 GTATCC
BsaAI 1930 CACGTA [CT]AC^GT[GA]
2534 CACGTG
BseRI 136 GAGGAG GAGGAG(10/8)
476 GAGGAG
BsiHKAI 324 GTGCAC G[AT]GC[AT]^C
2463 GTGCTC
BsmFI 155 GGGAC GGGAC(10/14)
2220 GGGAC
BsoBI 47 CTCGGG C^[CT]CG[GA]G
133 CCCGAG
BspHI 873 TCATGA T^CATGA
1701 TCATGA
BspLU11I 1425 ACATGT A^CATGT
2648 ACATGT
BstAPI 2257 GCATCTATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2841 GCAAAAGTTGC
BstEII 745 GGTGACC G^GT[ACGT]ACC
1285 GGTGACC
BstPI 745 GGTGACC G^GT[ACGT]ACC
1285 GGTGACC
BstUI 58 CGCG CG^CG
219 CGCG
BstXI 1275 CCAAGAATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1858 CCAAGACCATGG
EcoO65I 745 GGTGACC G^GT[ACGT]ACC
1285 GGTGACC
HincII 1429 GTTGAC GT[CT]^[GA]AC
2945 GTCAAC
HindII 1429 GTTGAC GT[CT]^[GA]AC
2945 GTCAAC
MluNI 1389 TGGCCA TGG^CCA
2643 TGGCCA
MscI 1389 TGGCCA TGG^CCA
2643 TGGCCA
MvnI 58 CGCG CG^CG
219 CGCG
PvuII 167 CAGCTG CAG^CTG
2621 CAGCTG
RcaI 873 TCATGA T^CATGA
1701 TCATGA
SfcI 678 CTACAG C^T[GA][CT]AG
1205 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AciI AfaI AflIII AluI AvaII
BanI BbvI BcnI BfaI BmyI
BpmI BsaBI BsaJI BsgI BsiYI
BslI BsmAI BsmI Bsp1286I Bsp1407I
BspMI BsrDI BsrGI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII EaeI EarI EcoRII
EcoRV EcoT14I Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SspBI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I