Restriction map for
pFN21AE2111
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# NO CUT ENZYMES ( 96 enzymes) #
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Acc65I AclI AcsI AgeI Alw44I
Aor51HI ApaLI ApoI AseI AsnI
Asp700I Asp718I AspI AvrII BbeI
BcgI BclI BfaI BglII BlnI
BlpI Bpu1102I BsaBI BsiWI Bsp1407I
BspLU11I BsrDI BsrGI Bst1107I BstBI
BstEII BstPI BstZ17I CelII CpoI
DraI DraIII Eco47III EcoO65I EcoRI
EcoRV EcoT22I FbaI HindIII HpaI
KasI KpnI KspI MaeI MamI
MfeI MluI MunI NarI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmeI PshBI Psp1406I PstI PvuI
RsrII SacII SalI SbfI ScaI
SexAI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tsp509I
Tth111I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AatII 2135 GACGTC GACGT^C
AccI 848 GTAGAC GT^[AC][GT]AC
AccIII 2036 TCCGGA T^CCGGA
AcyI 2135 GACGTC G[GA]^CG[CT]C
AflII 604 CTTAAG C^TTAAG
AflIII 1599 ACACGT A^C[GA][CT]GT
AhdI 991 GACACCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 214 CCTGAGG CC^T[ACGT]AGG
AscI 2127 GGCGCGCC GG^CGCGCC
AspEI 991 GACACCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1247 TGCGCA TGC^GCA
BbrPI 1492 CACGTG CAC^GTG
BciVI 706 GGATAC GTATCC(6/5)
BfrI 604 CTTAAG C^TTAAG
BsaHI 2135 GACGTC G[GA]^CG[CT]C
BsaI 837 GGTCTC GGTCTC(1/5)
BsaWI 2036 TCCGGA [AT]^CCGG[AT]
BseAI 2036 TCCGGA T^CCGGA
BsiEI 1577 CGGCCG CG[GA][CT]^CG
BsmBI 776 GAGACG CGTCTC(1/5)
BsmI 267 GCATTC GAATGC(1/-1)
BspDI 529 ATCGAT AT^CGAT
BspEI 2036 TCCGGA T^CCGGA
BsrBI 1231 GAGCGG CCGCTC(-3/-3)
BssHII 2128 GCGCGC G^CGCGC
BssSI 1387 CACGAG CACGAG(-5/-1)
BstAPI 267 GCATTCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 214 CCTGAGG CC^T[ACGT]AGG
ClaI 529 ATCGAT AT^CGAT
DrdI 2419 GACGAGGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1577 CGGCCG C^GGCCG
Eam1105I 991 GACACCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1577 CGGCCG C^GGCCG
Eco52I 1577 CGGCCG C^GGCCG
Eco81I 214 CCTGAGG CC^T[ACGT]AGG
EcoNI 315 CCTGAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 1247 TGCGCA TGC^GCA
Hin1I 2135 GACGTC G[GA]^CG[CT]C
HincII 901 GTCAAC GT[CT]^[GA]AC
HindII 901 GTCAAC GT[CT]^[GA]AC
MroI 2036 TCCGGA T^CCGGA
NcoI 1710 CCATGG C^CATGG
PmaCI 1492 CACGTG CAC^GTG
PmlI 1492 CACGTG CAC^GTG
PpuMI 1720 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 1997 GACCCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 1836 GAAGAGC GCTCTTC(1/4)
SfiI 1289 GGCCCCCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
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# TWO CUT ENZYMES ( 16 enzymes) #
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ApaI 1288 GGGCCC GGGCC^C
1670 GGGCCC
BalI 1467 TGGCCA TGG^CCA
1496 TGGCCA
BbsI 794 GAAGAC GAAGAC(2/6)
1956 GTCTTC
BbvCI 2029 CCTCAGC CCTCAGC(-5/-2)
1434 GCTGAGG
BglI 1290 GCCCCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1816 GCCAAGAAGGC
BpuAI 794 GAAGAC GAAGAC(2/6)
1956 GTCTTC
BspHI 515 TCATGA T^CATGA
1983 TCATGA
BstXI 669 CCAGAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1452 CCAGTCGCCTGG
DsaI 1710 CCATGG C^C[GA][CT]GG
1950 CCACGG
FseI 1578 GGCCGGCC GGCCGG^CC
2022 GGCCGGCC
HgaI 778 GACGC GACGC(5/10)
1573 GACGC
MluNI 1467 TGGCCA TGG^CCA
1496 TGGCCA
MscI 1467 TGGCCA TGG^CCA
1496 TGGCCA
RcaI 515 TCATGA T^CATGA
1983 TCATGA
SacI 672 GAGCTC GAGCT^C
1171 GAGCTC
SmlI 604 CTTAAG C^T[CT][GA]AG
2008 CTTGAG
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# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
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AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BamHI
BanI BanII BbvI BcnI BmyI
BpmI BsaAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV PflMI
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
Tru9I TseI Tsp45I TspRI TthHB8I
Van91I XcmI XhoII