Restriction map for
pFN21AB7963
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI Alw44I AlwNI
AocI ApaI ApaLI AscI AseI
AsnI Asp718I AviII AvrII BamHI
BanII BbeI BbrPI BbvCI BciVI
BclI BfaI BfrI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BseRI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI EagI EarI EclXI
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HgaI Hin1I HpaI KasI KpnI
Ksp632I KspI MaeI MaeIII MamI
MfeI MluI MroI MunI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tsp45I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AccI 12 GTCTAC GT^[AC][GT]AC
AccII 472 CGCG CG^CG
AcsI 190 AAATTC [GA]^AATT[CT]
AhdI 841 GACTACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 23 GGATC GGATC(4/5)
Aor51HI 497 AGCGCT AGC^GCT
ApoI 190 AAATTC [GA]^AATT[CT]
AspEI 841 GACTACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 854 GTGCTC G[AT]GC[AT]^C
AspI 862 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 346 CTCGGG C^[CT]CG[GA]G
BanI 724 GGCACC G^G[CT][GA]CC
BcgI 437 GCACCTTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 543 CCGGG CC^[GC]GG
BglI 476 GCCTGGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 210 AGATCT A^GATCT
BmyI 854 GTGCTC G[AGT]GC[ACT]^C
BpmI 430 CTGGAG CTGGAG(16/14)
BsgI 466 GTGCAG GTGCAG(16/14)
BsiHKAI 854 GTGCTC G[AT]GC[AT]^C
BsmI 746 GCATTC GAATGC(1/-1)
BsoBI 346 CTCGGG C^[CT]CG[GA]G
Bsp1286I 854 GTGCTC G[AGT]GC[ACT]^C
BspHI 858 TCATGA T^CATGA
BsrDI 99 CATTGC GCAATG(2/0)
BssSI 523 CTCGTG CACGAG(-5/-1)
BstF5I 384 CATCC GGATG(2/0)
BstUI 472 CGCG CG^CG
BstYI 210 AGATCT [GA]^GATC[CT]
DraII 42 GGGGCCT [GA]G^G[ACGT]CC[CT]
Eam1105I 841 GACTACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 497 AGCGCT AGC^GCT
EcoO109I 42 GGGGCCT [GA]G^G[ACGT]CC[CT]
FauI 126 GCGGG CCCGC(4/6)
FokI 384 CATCC GGATG(9/13)
HaeII 497 AGCGCT [GA]GCGC^[CT]
HindIII 200 AAGCTT A^AGCTT
MflI 210 AGATCT [GA]^GATC[CT]
MseI 821 TTAA T^TAA
MslI 772 CATGGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 124 CAGCGG C[AC]G^C[GT]G
MvnI 472 CGCG CG^CG
NaeI 259 GCCGGC GCC^GGC
NciI 543 CCGGG CC^[GC]GG
NgoMIV 259 GCCGGC G^CCGGC
NspI 275 ACATGC [GA]CATG^[CT]
PflFI 862 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 362 CTGCAG CTGCA^G
RcaI 858 TCATGA T^CATGA
SfaNI 687 GATGC GCATC(5/9)
SfcI 362 CTGCAG C^T[GA][CT]AG
TaqI 503 TCGA T^CGA
Tru9I 821 TTAA T^TAA
Tsp509I 191 AATT ^AATT
TspRI 777 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 862 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 503 TCGA T^CGA
XcmI 419 CCATGGCCAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 210 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AfaI 92 GTAC GT^AC
618 GTAC
AluI 58 AGCT AG^CT
201 AGCT
BbsI 327 GAAGAC GAAGAC(2/6)
672 GAAGAC
BbvI 468 GCAGC GCAGC(8/12)
567 GCAGC
BpuAI 327 GAAGAC GAAGAC(2/6)
672 GAAGAC
BsaI 352 GAGACC GGTCTC(1/5)
697 GAGACC
BsmFI 379 GGGAC GGGAC(10/14)
737 GGGAC
BsrI 834 ACTGG ACTGG(1/-1)
863 ACTGG
DdeI 282 CTCAG C^T[ACGT]AG
627 CTCAG
HincII 232 GTTGAC GT[CT]^[GA]AC
838 GTTGAC
HindII 232 GTTGAC GT[CT]^[GA]AC
838 GTTGAC
MaeII 230 ACGT A^CGT
665 ACGT
PflMI 299 CCATGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
644 CCATGGTCTGG
PleI 367 GACTC GAGTC(4/5)
338 GAGTC
RsaI 92 GTAC GT^AC
618 GTAC
SmlI 54 CTCAAG C^T[CT][GA]AG
217 CTCAAG
TseI 468 GCAGC G^C[AT]GC
567 GCAGC
Van91I 299 CCATGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
644 CCATGGTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI Asp700I AvaII BalI BsaJI
BsiYI BslI BsmAI BsrFI BssKI
BstNI Cac8I CfoI Cfr10I Cfr13I
DpnII DsaI EaeI EcoRII EcoT14I
Fnu4HI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MboI
MboII MluNI MnlI MscI MspI
MvaI MwoI NcoI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI StyI
TfiI XmnI