Restriction map for
pFN21AE3201
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BalI BamHI BbrPI BbvCI
BclI BcnI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaWI
BseAI BsgI BsiEI BsiWI BsmI
BspEI BspMI BsrBI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII Cfr10I CpoI DraIII EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoT22I FauI FbaI
FseI FspI HincII HindII HpaI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MunI MvnI
NaeI NciI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SwaI Tth111I Van91I XbaI XcmI
XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AatII 526 GACGTC GACGT^C
AflIII 710 ACATGT A^C[GA][CT]GT
AlwNI 562 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 1021 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 362 GTGCTC G[AT]GC[AT]^C
AvaI 365 CTCGAG C^[CT]CG[GA]G
BanI 24 GGCGCC G^G[CT][GA]CC
BanII 322 GAGCCC G[GA]GC[CT]^C
BbeI 24 GGCGCC GGCGC^C
BcgI 531 CGATACAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1120 GGATAC GTATCC(6/5)
BfaI 37 CTAG C^TAG
BglII 1169 AGATCT A^GATCT
BpmI 1037 CTGGAG CTGGAG(16/14)
BsiHKAI 362 GTGCTC G[AT]GC[AT]^C
BsiYI 1068 CCCAGGTCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 1068 CCCAGGTCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 564 GGGAC GGGAC(10/14)
BsoBI 365 CTCGAG C^[CT]CG[GA]G
Bsp1407I 894 TGTACA T^GTACA
BspDI 425 ATCGAT AT^CGAT
BspHI 144 TCATGA T^CATGA
BspLU11I 710 ACATGT A^CATGT
BsrDI 166 CATTGC GCAATG(2/0)
BsrGI 894 TGTACA T^GTACA
ClaI 425 ATCGAT AT^CGAT
DraI 1159 TTTAAA TTT^AAA
DrdI 33 GACCCTAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 8 CCATGG C^C[GA][CT]GG
EaeI 5 CGGCCA [CT]^GGCC[GA]
EcoRI 440 GAATTC G^AATTC
EcoRV 186 GATATC GAT^ATC
EcoT14I 8 CCATGG C^C[AT][AT]GG
HaeII 24 GGCGCC [GA]GCGC^[CT]
HapII 542 CCGG C^CGG
HgaI 1391 GACGC GACGC(5/10)
HindIII 409 AAGCTT A^AGCTT
HpaII 542 CCGG C^CGG
KasI 24 GGCGCC G^GCGCC
MaeI 37 CTAG C^TAG
MaeIII 1013 GTGAC ^GT[ACGT]AC
MslI 621 CATAGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 542 CCGG C^CGG
NarI 24 GGCGCC GG^CGCC
NcoI 8 CCATGG C^CATGG
NdeI 1198 CATATG CA^TATG
PaeR7I 365 CTCGAG C^TCGAG
PstI 635 CTGCAG CTGCA^G
RcaI 144 TCATGA T^CATGA
SfoI 24 GGCGCC GGC^GCC
SspBI 894 TGTACA T^GTACA
SspI 314 AATATT AAT^ATT
StyI 8 CCATGG C^C[AT][AT]GG
Tsp45I 1013 GTGAC ^GT[GC]AC
XhoI 365 CTCGAG C^TCGAG
XmnI 1021 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AvaII 1033 GGACC G^G[AT]CC
1246 GGTCC
BmyI 322 GAGCCC G[AGT]GC[ACT]^C
362 GTGCTC
BsaI 13 GAGACC GGTCTC(1/5)
794 GAGACC
BsmBI 19 GAGACG CGTCTC(1/5)
1356 GAGACG
Bsp1286I 322 GAGCCC G[AGT]GC[ACT]^C
362 GTGCTC
BstYI 93 GGATCT [GA]^GATC[CT]
1169 AGATCT
Cac8I 131 GCCAGC GC[ACGT]^[ACGT]GC
407 GCAAGC
CfoI 25 GCGC GCG^C
394 GCGC
DraII 1032 AGGACCT [GA]G^G[ACGT]CC[CT]
1245 GGGTCCT
EcoO109I 1032 AGGACCT [GA]G^G[ACGT]CC[CT]
1245 GGGTCCT
HhaI 25 GCGC GCG^C
394 GCGC
HinP1I 25 GCGC G^CGC
394 GCGC
HphI 1012 GGTGA GGTGA(8/7)
1048 GGTGA
MaeII 527 ACGT A^CGT
1016 ACGT
MflI 93 GGATCT [GA]^GATC[CT]
1169 AGATCT
NspI 710 ACATGT [GA]CATG^[CT]
1204 GCATGT
PpuMI 1032 AGGACCT [GA]G^G[AT]CC[CT]
1245 GGGTCCT
PvuII 536 CAGCTG CAG^CTG
698 CAGCTG
SfaNI 382 GATGC GCATC(5/9)
949 GATGC
SfcI 635 CTGCAG C^T[GA][CT]AG
1226 CTATAG
SmlI 307 CTCAAG C^T[CT][GA]AG
365 CTCGAG
StuI 293 AGGCCT AGG^CCT
965 AGGCCT
XhoII 93 GGATCT [GA]^GATC[CT]
1169 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI ApoI BbsI BbvI BpuAI
BsaHI BsaJI BseRI BsmAI BsrI
BssKI BstF5I BstNI Cfr13I DdeI
DpnII EarI EcoRII Fnu4HI FokI
HaeIII Hin1I HinfI ItaI Ksp632I
MboI MboII MnlI MseI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I