Restriction map for
pFN21AB9645
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# NO CUT ENZYMES ( 69 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AscI AseI AsnI Asp718I AviII
AvrII BbrPI BcgI BciVI BfrI
BglII BlnI BsaAI BsaBI BseAI
BsmI BspDI BspEI BspLU11I BsrBI
BssHII Bst1107I BstXI BstZ17I ClaI
CpoI DraIII EcoRI EcoRV EcoT22I
FseI FspI HindIII HpaI KpnI
MamI MfeI MluI MroI MunI
NdeI NotI NruI NsiI PacI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SalI ScaI
SexAI SfiI SgrAI SnaBI SpeI
SspI StuI SwaI XbaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 495 GACGTC GACGT^C
AflIII 1769 ACGTGT A^C[GA][CT]GT
AgeI 2505 ACCGGT A^CCGGT
AhdI 2494 GACGAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1948 CCTGAGG CC^T[ACGT]AGG
Asp700I 2804 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2494 GACGAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 175 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 159 GGATCC G^GATCC
BbvCI 828 CCTCAGC CCTCAGC(-5/-2)
BclI 842 TGATCA T^GATCA
BfaI 118 CTAG C^TAG
BglI 1093 GCCCGCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
Bsp1407I 2186 TGTACA T^GTACA
BspHI 2120 TCATGA T^CATGA
BsrDI 5 GCAATG GCAATG(2/0)
BsrGI 2186 TGTACA T^GTACA
BssSI 378 CACGAG CACGAG(-5/-1)
BstBI 2907 TTCGAA TT^CGAA
Bsu36I 1948 CCTGAGG CC^T[ACGT]AGG
DraI 270 TTTAAA TTT^AAA
EagI 1646 CGGCCG C^GGCCG
Eam1105I 2494 GACGAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1646 CGGCCG C^GGCCG
Eco52I 1646 CGGCCG C^GGCCG
Eco81I 1948 CCTGAGG CC^T[ACGT]AGG
FbaI 842 TGATCA T^GATCA
HincII 1813 GTCAAC GT[CT]^[GA]AC
HindII 1813 GTCAAC GT[CT]^[GA]AC
KspI 1334 CCGCGG CCGC^GG
MaeI 118 CTAG C^TAG
NcoI 1778 CCATGG C^CATGG
NheI 117 GCTAGC G^CTAGC
NspV 2907 TTCGAA TT^CGAA
PflFI 175 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2505 ACCGGT A^CCGGT
PvuII 396 CAGCTG CAG^CTG
RcaI 2120 TCATGA T^CATGA
SacII 1334 CCGCGG CCGC^GG
SbfI 2562 CCTGCAGG CCTGCA^GG
SfuI 2907 TTCGAA TT^CGAA
SmaI 1620 CCCGGG CCC^GGG
SphI 2204 GCATGC GCATG^C
Sse8387I 2562 CCTGCAGG CCTGCA^GG
SspBI 2186 TGTACA T^GTACA
Tth111I 175 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1620 CCCGGG C^CCGGG
XmaI 1620 CCCGGG C^CCGGG
XmnI 2804 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 1441 AAATTT [GA]^AATT[CT]
2737 AAATTC
Alw44I 652 GTGCAC G^TGCAC
2644 GTGCAC
AlwNI 934 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
2685 CAGCCACTG
Aor51HI 737 AGCGCT AGC^GCT
2104 AGCGCT
ApaLI 652 GTGCAC G^TGCAC
2644 GTGCAC
ApoI 1441 AAATTT [GA]^AATT[CT]
2737 AAATTC
BlpI 257 GCTTAGC GC^T[ACGT]AGC
2100 GCTGAGC
Bpu1102I 257 GCTTAGC GC^T[ACGT]AGC
2100 GCTGAGC
BsaI 1833 GAGACC GGTCTC(1/5)
2593 GAGACC
BsiEI 1646 CGGCCG CG[GA][CT]^CG
2259 CGACCG
BsiWI 626 CGTACG C^GTACG
1028 CGTACG
BstEII 1173 GGTCACC G^GT[ACGT]ACC
2031 GGTCACC
BstPI 1173 GGTCACC G^GT[ACGT]ACC
2031 GGTCACC
CelII 257 GCTTAGC GC^T[ACGT]AGC
2100 GCTGAGC
DrdI 826 GACCTCAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2418 GACGCCCAAGTC
Eco47III 737 AGCGCT AGC^GCT
2104 AGCGCT
EcoO65I 1173 GGTCACC G^GT[ACGT]ACC
2031 GGTCACC
MseI 271 TTAA T^TAA
2299 TTAA
NspI 791 GCATGT [GA]CATG^[CT]
2204 GCATGC
PaeR7I 143 CTCGAG C^TCGAG
2580 CTCGAG
SacI 321 GAGCTC GAGCT^C
1854 GAGCTC
SapI 1851 GAAGAGC GCTCTTC(1/4)
134 GCTCTTC
TfiI 2341 GATTC G^A[AT]TC
2886 GATTC
Tru9I 271 TTAA T^TAA
2299 TTAA
Tsp509I 1442 AATT ^AATT
2738 AATT
XhoI 143 CTCGAG C^TCGAG
2580 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI ApaI AspHI AvaI AvaII
BalI BanI BanII BbeI BbsI
BbvI BcnI BmyI BpmI BpuAI
BsaHI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NdeII
NgoMIV NlaIII NlaIV PflMI PleI
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmlI
StyI TaqI TseI Tsp45I TspRI
TthHB8I Van91I XcmI XhoII