Restriction map for
pFN21AB7806
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# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AflIII AgeI AhdI AocI Aor51HI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BamHI BbrPI BbvCI
BciVI BclI BfaI BfrI BglII
BlnI BsaBI BsaI BsiWI BspDI
BspHI BspLU11I BsrDI Bst1107I BstBI
BstEII BstPI BstYI BstZ17I Bsu36I
ClaI CpoI DraI DrdI Eam1105I
EarI Eco47III Eco81I EcoO65I EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI Ksp632I
MaeI MamI MfeI MflI MluI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI SapI SbfI ScaI SexAI
SfuI SgrAI SmlI SnaBI SpeI
Sse8387I SspI SwaI TfiI Tth111I
XbaI XhoI XhoII
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccIII 1374 TCCGGA T^CCGGA
AcsI 727 GAATTC [GA]^AATT[CT]
AcyI 1049 GGCGCC G[GA]^CG[CT]C
Alw44I 1427 GTGCAC G^TGCAC
ApaLI 1427 GTGCAC G^TGCAC
ApoI 727 GAATTC [GA]^AATT[CT]
AscI 48 GGCGCGCC GG^CGCGCC
Asp700I 723 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI 1049 GGCGCC GGCGC^C
BbsI 400 GAAGAC GAAGAC(2/6)
BcgI 87 CGATCCCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 1606 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1606 GCTCAGC GC^T[ACGT]AGC
BpuAI 400 GAAGAC GAAGAC(2/6)
BsaHI 1049 GGCGCC G[GA]^CG[CT]C
BseAI 1374 TCCGGA T^CCGGA
BsmAI 22 GAGAC GTCTC(1/5)
BsmBI 22 GAGACG CGTCTC(1/5)
BsmI 1475 GCATTC GAATGC(1/-1)
Bsp1407I 1586 TGTACA T^GTACA
BspEI 1374 TCCGGA T^CCGGA
BspMI 905 ACCTGC ACCTGC(4/8)
BsrBI 586 GAGCGG CCGCTC(-3/-3)
BsrGI 1586 TGTACA T^GTACA
BssHII 49 GCGCGC G^CGCGC
BssSI 853 CTCGTG CACGAG(-5/-1)
BstAPI 72 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 1606 GCTCAGC GC^T[ACGT]AGC
DraIII 1258 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 727 GAATTC G^AATTC
HaeII 1049 GGCGCC [GA]GCGC^[CT]
Hin1I 1049 GGCGCC G[GA]^CG[CT]C
KasI 1049 GGCGCC G^GCGCC
KspI 549 CCGCGG CCGC^GG
MroI 1374 TCCGGA T^CCGGA
MseI 686 TTAA T^TAA
NarI 1049 GGCGCC GG^CGCC
NcoI 1451 CCATGG C^CATGG
SacI 1009 GAGCTC GAGCT^C
SacII 549 CCGCGG CCGC^GG
SfoI 1049 GGCGCC GGC^GCC
SphI 1103 GCATGC GCATG^C
SspBI 1586 TGTACA T^GTACA
StuI 542 AGGCCT AGG^CCT
TaqI 704 TCGA T^CGA
Tru9I 686 TTAA T^TAA
Tsp45I 835 GTGAC ^GT[GC]AC
Tsp509I 728 AATT ^AATT
TthHB8I 704 TCGA T^CGA
XcmI 1053 CCAGAGCTTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 723 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwI 88 GATCC GGATC(4/5)
1481 GGATC
BalI 794 TGGCCA TGG^CCA
1448 TGGCCA
BsaAI 628 CACGTA [CT]AC^GT[GA]
826 TACGTG
BsaWI 1374 TCCGGA [AT]^CCGG[AT]
1702 ACCGGA
BsrFI 1046 GCCGGC [GA]^CCGG[CT]
1614 GCCGGC
BstXI 566 CCAGGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1053 CCAGAGCTTTGG
Cfr10I 1046 GCCGGC [GA]^CCGG[CT]
1614 GCCGGC
EcoNI 405 CCTCGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
852 CCTCGTGGAGG
EcoT14I 893 CCAAGG C^C[AT][AT]GG
1451 CCATGG
HgaI 24 GACGC GACGC(5/10)
274 GACGC
MaeIII 835 GTGAC ^GT[ACGT]AC
998 GTAAC
MluNI 794 TGGCCA TGG^CCA
1448 TGGCCA
MscI 794 TGGCCA TGG^CCA
1448 TGGCCA
MslI 1554 CATCAGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1560 CATGTCGGTG
NaeI 1046 GCCGGC GCC^GGC
1614 GCCGGC
NgoMIV 1046 GCCGGC G^CCGGC
1614 GCCGGC
NspI 1103 GCATGC [GA]CATG^[CT]
1559 GCATGT
PflMI 54 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1257 CCACCCTGTGG
PpuMI 710 GGGACCC [GA]G^G[AT]CC[CT]
803 AGGTCCT
StyI 893 CCAAGG C^C[AT][AT]GG
1451 CCATGG
Van91I 54 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1257 CCACCCTGTGG
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# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
ApaI AspHI AvaI AvaII BanI
BanII BbvI BcnI BglI BmyI
BpmI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EagI
EclXI Eco52I EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeII MboI MboII MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SfiI SmaI TseI
TspRI XmaCI XmaI