Restriction map for
pFN21AE3501
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# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII AccI AclI AgeI AhdI
Aor51HI ApaI AscI AspEI AviII
AvrII BalI BbeI BbrPI BcgI
BclI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsiEI BsiWI Bsp1407I
BspDI BspHI BsrFI BsrGI BssHII
Bst1107I BstZ17I CelII Cfr10I ClaI
CpoI DraI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoT22I FbaI
FseI FspI HaeII KasI KspI
MfeI MluI MluNI MscI MunI
NaeI NarI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI StuI
SwaI XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 1578 GGTACC G^GTACC
AccIII 553 TCCGGA T^CCGGA
AcyI 81 GGCGTC G[GA]^CG[CT]C
AflIII 2747 ACATGT A^C[GA][CT]GT
Alw44I 993 GTGCAC G^TGCAC
ApaLI 993 GTGCAC G^TGCAC
AseI 2703 ATTAAT AT^TAAT
AsnI 2703 ATTAAT AT^TAAT
Asp718I 1578 GGTACC G^GTACC
AspI 66 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 150 GGATCC G^GATCC
BbvCI 1503 GCTGAGG CCTCAGC(-5/-2)
BciVI 1277 GTATCC GTATCC(6/5)
BsaHI 81 GGCGTC G[GA]^CG[CT]C
BsaI 119 GAGACC GGTCTC(1/5)
BseAI 553 TCCGGA T^CCGGA
BsgI 2270 CTGCAC GTGCAG(16/14)
BsmBI 3275 GAGACG CGTCTC(1/5)
BsmI 3087 GCATTC GAATGC(1/-1)
BspEI 553 TCCGGA T^CCGGA
BspLU11I 2747 ACATGT A^CATGT
BsrBI 549 CCGCTC CCGCTC(-3/-3)
BsrDI 1876 GCAATG GCAATG(2/0)
BssSI 229 CACGAG CACGAG(-5/-1)
BstBI 3245 TTCGAA TT^CGAA
DraII 1145 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 2599 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 66 GACTCCGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 219 CGGCCA [CT]^GGCC[GA]
EcoO109I 1145 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 2419 GAATTC G^AATTC
EcoRV 755 GATATC GAT^ATC
FauI 597 GCGGG CCCGC(4/6)
Hin1I 81 GGCGTC G[GA]^CG[CT]C
HincII 523 GTTAAC GT[CT]^[GA]AC
HindII 523 GTTAAC GT[CT]^[GA]AC
HpaI 523 GTTAAC GTT^AAC
KpnI 1578 GGTACC GGTAC^C
MroI 553 TCCGGA T^CCGGA
NdeI 1553 CATATG CA^TATG
NspV 3245 TTCGAA TT^CGAA
PflFI 66 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1582 CCAGACTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1145 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 2351 GACGACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2703 ATTAAT AT^TAAT
SacI 694 GAGCTC GAGCT^C
SapI 696 GCTCTTC GCTCTTC(1/4)
ScaI 1489 AGTACT AGT^ACT
SfuI 3245 TTCGAA TT^CGAA
SspI 2155 AATATT AAT^ATT
Tth111I 66 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1582 CCAGACTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AflII 711 CTTAAG C^TTAAG
2371 CTTAAG
AvaI 310 CTCGGG C^[CT]CG[GA]G
674 CTCGGG
BbsI 2376 GTCTTC GAAGAC(2/6)
3455 GTCTTC
BfrI 711 CTTAAG C^TTAAG
2371 CTTAAG
BpuAI 2376 GTCTTC GAAGAC(2/6)
3455 GTCTTC
BsaBI 1300 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2328 GATCCGAATC
BsoBI 310 CTCGGG C^[CT]CG[GA]G
674 CTCGGG
BspMI 290 ACCTGC ACCTGC(4/8)
2274 ACCTGC
BstAPI 237 GCAACAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3553 GCAGCCTTTGC
BstYI 150 GGATCC [GA]^GATC[CT]
2327 AGATCC
HgaI 82 GCGTC GACGC(5/10)
168 GCGTC
HindIII 439 AAGCTT A^AGCTT
1769 AAGCTT
MaeII 275 ACGT A^CGT
3062 ACGT
MamI 1300 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2328 GATCCGAATC
MflI 150 GGATCC [GA]^GATC[CT]
2327 AGATCC
SmlI 711 CTTAAG C^T[CT][GA]AG
2371 CTTAAG
SphI 663 GCATGC GCATG^C
705 GCATGC
XhoII 150 GGATCC [GA]^GATC[CT]
2327 AGATCC
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# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI AocI ApoI Asp700I
AspHI AvaII BanI BanII BbvI
BcnI BfaI BmyI BpmI BsaJI
BsaWI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstEII BstF5I BstNI BstPI BstUI
BstXI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DsaI EarI Eco81I
EcoO65I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XmnI