##################################
# NO CUT ENZYMES (  141 enzymes) #
##################################

AatII       AccIII      AclI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
AspEI       AspHI       AspI        AvaI        AvrII       
BalI        BamHI       BanII       BbeI        BbrPI       
BbvCI       BcgI        BciVI       BfrI        BglI        
BglII       BlnI        BlpI        BmyI        Bpu1102I    
BsaAI       BsaI        BseAI       BsgI        BsiEI       
BsiHKAI     BsoBI       Bsp1286I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrFI       BssHII      
BssSI       BstBI       BstEII      BstPI       BstXI       
BstYI       CelII       Cfr10I      ClaI        CpoI        
DraII       DraIII      DrdI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
EcoNI       EcoO109I    EcoO65I     EcoRV       FseI        
HaeII       HincII      HindII      HpaI        KasI        
Ksp632I     KspI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MspA1I      MunI        
NaeI        NarI        NgoMIV      NheI        NotI        
NruI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspI        SwaI        Tth111I     Van91I      
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

Acc65I        200  GGTACC               G^GTACC
AccI         1196  GTATAC               GT^[AC][GT]AC
AccII        1691  CGCG                 CG^CG
AcyI          146  GACGCC               G[GA]^CG[CT]C
AocI          796  CCTGAGG              CC^T[ACGT]AGG
Asp718I       200  GGTACC               G^GTACC
AviII        1869  TGCGCA               TGC^GCA
BanI          200  GGTACC               G^G[CT][GA]CC
BclI          363  TGATCA               T^GATCA
BsaBI         943  GATGTACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         146  GACGCC               G[GA]^CG[CT]C
BsiWI        1817  CGTACG               C^GTACG
BsmBI         144  GAGACG               CGTCTC(1/5)
BspMI         185  ACCTGC               ACCTGC(4/8)
Bst1107I     1196  GTATAC               GTA^TAC
BstAPI       1742  GCAAGGACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI        1691  CGCG                 CG^CG
BstZ17I      1196  GTATAC               GTA^TAC
Bsu36I        796  CCTGAGG              CC^T[ACGT]AGG
Cac8I        1784  GCGTGC               GC[ACGT]^[ACGT]GC
DraI          597  TTTAAA               TTT^AAA
Eco81I        796  CCTGAGG              CC^T[ACGT]AGG
FbaI          363  TGATCA               T^GATCA
FspI         1869  TGCGCA               TGC^GCA
HgaI          146  GACGC                GACGC(5/10)
Hin1I         146  GACGCC               G[GA]^CG[CT]C
KpnI          200  GGTACC               GGTAC^C
MamI          943  GATGTACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI         1691  CGCG                 CG^CG
NdeI         1680  CATATG               CA^TATG
PstI           44  CTGCAG               CTGCA^G
TaqI         2278  TCGA                 T^CGA
TthHB8I      2278  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AvaII          29  GGTCC                G^G[AT]CC
              132  GGACC               
BbsI          919  GTCTTC               GAAGAC(2/6)
             1507  GTCTTC              
BcnI           33  CCCGG                CC^[GC]GG
             1049  CCGGG               
BpuAI         919  GTCTTC               GAAGAC(2/6)
             1507  GTCTTC              
BsaWI        1216  ACCGGA               [AT]^CCGG[AT]
             1468  ACCGGA              
BseRI         115  GAGGAG               GAGGAG(10/8)
              799  GAGGAG              
BsmAI         144  GAGAC                GTCTC(1/5)
             1728  GAGAC               
DsaI           25  CCATGG               C^C[GA][CT]GG
              253  CCATGG              
NciI           33  CCCGG                CC^[GC]GG
             1049  CCGGG               
NcoI           25  CCATGG               C^CATGG
              253  CCATGG              
PleI           16  GACTC                GAGTC(4/5)
              563  GACTC               
SfcI           44  CTGCAG               C^T[GA][CT]AG
              177  CTACAG              


#########################################
# 3 OR MORE CUT ENZYMES (   72 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwNI       
ApoI        Asp700I     BbvI        BfaI        BpmI        
BsaJI       BsiYI       BslI        BsmFI       BsmI        
Bsp1407I    BsrDI       BsrGI       BsrI        BssKI       
BstF5I      BstNI       CfoI        Cfr13I      DdeI        
DpnII       EcoRI       EcoRII      EcoT14I     EcoT22I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HindIII     HinfI       HpaII       
HphI        ItaI        MaeI        MaeII       MaeIII      
MboI        MboII       MnlI        MseI        MslI        
MspI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       NsiI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SspBI       StuI        StyI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       XmnI