Restriction map for
pFN21AE3684
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AflIII AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI AspEI AspI
AviII AvrII BalI BbrPI BbsI
BclI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BseAI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstZ17I CelII ClaI CpoI
DraI DrdI Eam1105I Eco47III EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI MamI
MfeI MluI MluNI MroI MscI
MseI MunI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI ScaI SexAI
SfaNI SfiI SfuI SgrAI SmlI
SnaBI SpeI SphI SspBI SspI
SwaI Tru9I Tth111I XbaI XcmI
XhoI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 1397 GGTACC G^GTACC
AcsI 933 GAATTT [GA]^AATT[CT]
AgeI 1394 ACCGGT A^CCGGT
AlwNI 1042 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 467 CCTCAGG CC^T[ACGT]AGG
ApoI 933 GAATTT [GA]^AATT[CT]
Asp718I 1397 GGTACC G^GTACC
BamHI 166 GGATCC G^GATCC
BbeI 86 GGCGCC GGCGC^C
BbvCI 948 GCTGAGG CCTCAGC(-5/-2)
BcgI 368 CGAAACCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 801 GTATCC GTATCC(6/5)
BglI 1411 GCCCTGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 341 GAGACC GGTCTC(1/5)
BsaWI 1394 ACCGGT [AT]^CCGG[AT]
BsiEI 1359 CGGCCG CG[GA][CT]^CG
BsmBI 686 CGTCTC CGTCTC(1/5)
BstEII 1287 GGTCACC G^GT[ACGT]ACC
BstPI 1287 GGTCACC G^GT[ACGT]ACC
BstYI 166 GGATCC [GA]^GATC[CT]
Bsu36I 467 CCTCAGG CC^T[ACGT]AGG
DraIII 1004 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1359 CGGCCG [CT]^GGCC[GA]
EagI 1359 CGGCCG C^GGCCG
EclXI 1359 CGGCCG C^GGCCG
Eco52I 1359 CGGCCG C^GGCCG
Eco81I 467 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1287 GGTCACC G^GT[ACGT]ACC
HaeII 86 GGCGCC [GA]GCGC^[CT]
HinfI 148 GAATC G^A[ACGT]TC
KasI 86 GGCGCC G^GCGCC
KpnI 1397 GGTACC GGTAC^C
KspI 82 CCGCGG CCGC^GG
MaeII 1073 ACGT A^CGT
MflI 166 GGATCC [GA]^GATC[CT]
NaeI 761 GCCGGC GCC^GGC
NarI 86 GGCGCC GG^CGCC
NgoMIV 761 GCCGGC G^CCGGC
NspI 797 GCATGT [GA]CATG^[CT]
PflMI 1003 CCACAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1394 ACCGGT A^CCGGT
PvuII 1052 CAGCTG CAG^CTG
SacI 291 GAGCTC GAGCT^C
SacII 82 CCGCGG CCGC^GG
SbfI 1413 CCTGCAGG CCTGCA^GG
SfoI 86 GGCGCC GGC^GCC
Sse8387I 1413 CCTGCAGG CCTGCA^GG
TfiI 148 GAATC G^A[AT]TC
Van91I 1003 CCACAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 166 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AcyI 86 GGCGCC G[GA]^CG[CT]C
1375 GACGCC
AfaI 1014 GTAC GT^AC
1398 GTAC
ApaI 330 GGGCCC GGGCC^C
680 GGGCCC
Asp700I 837 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
969 GAACCGCTTC
AspHI 291 GAGCTC G[AT]GC[AT]^C
724 GTGCTC
BanI 86 GGCGCC G^G[CT][GA]CC
1397 GGTACC
BfaI 1166 CTAG C^TAG
1387 CTAG
BsaHI 86 GGCGCC G[GA]^CG[CT]C
1375 GACGCC
BsgI 817 CTGCAC GTGCAG(16/14)
1222 CTGCAC
BsiHKAI 291 GAGCTC G[AT]GC[AT]^C
724 GTGCTC
BsmAI 341 GAGAC GTCTC(1/5)
687 GTCTC
BsrFI 761 GCCGGC [GA]^CCGG[CT]
1394 ACCGGT
BsrI 482 CCAGT ACTGG(1/-1)
1439 CCAGT
BstF5I 1149 CATCC GGATG(2/0)
12 GGATG
Cfr10I 761 GCCGGC [GA]^CCGG[CT]
1394 ACCGGT
EcoNI 21 CCTCCGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1035 CCTTCGGCAGG
FokI 1149 CATCC GGATG(9/13)
12 GGATG
HgaI 1200 GACGC GACGC(5/10)
1375 GACGC
Hin1I 86 GGCGCC G[GA]^CG[CT]C
1375 GACGCC
MaeI 1166 CTAG C^TAG
1387 CTAG
MaeIII 1169 GTGAC ^GT[ACGT]AC
1288 GTCAC
MslI 1020 CACTAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1324 CACGCTGATG
PpuMI 461 AGGACCC [GA]G^G[AT]CC[CT]
1142 GGGTCCC
PstI 1351 CTGCAG CTGCA^G
1414 CTGCAG
RsaI 1014 GTAC GT^AC
1398 GTAC
SapI 288 GAAGAGC GCTCTTC(1/4)
492 GAAGAGC
SfcI 1351 CTGCAG C^T[GA][CT]AG
1414 CTGCAG
SmaI 713 CCCGGG CCC^GGG
770 CCCGGG
StuI 1295 AGGCCT AGG^CCT
1418 AGGCCT
Tsp45I 1169 GTGAC ^GT[GC]AC
1288 GTCAC
Tsp509I 886 AATT ^AATT
934 AATT
XmaCI 713 CCCGGG C^CCGGG
770 CCCGGG
XmaI 713 CCCGGG C^CCGGG
770 CCCGGG
XmnI 837 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
969 GAACCGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AccII AciI AluI AlwI AvaI
AvaII BanII BbvI BcnI BmyI
BpmI BsaJI BseRI BsiYI BslI
BsmFI BsoBI Bsp1286I BssKI BstNI
BstUI BstXI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI Ksp632I MboI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI StyI TaqI
TseI TspRI TthHB8I