Restriction map for
pFN21AE3691
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI Asp700I
AspEI AspI AvaI AvrII BbrPI
BcgI BciVI BclI BcnI BglI
BglII BlnI BsaBI BsaI BsaWI
BseAI BsiEI BsiWI BsmAI BsmBI
BsoBI BspDI BspEI BspLU11I BsrBI
BsrFI BssHII Bst1107I BstAPI BstEII
BstPI BstUI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoT22I FauI
FbaI FseI HapII HpaII KspI
MamI MluI MroI MspI MvnI
NaeI NciI NdeI NgoMIV NheI
NotI NruI NsiI PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI Psp1406I PstI PvuI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Tth111I Van91I XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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Acc65I 1136 GGTACC G^GTACC
AciI 1184 GCGG CCGC(-3/-1)
AcyI 1554 GGCGCC G[GA]^CG[CT]C
AflII 745 CTTAAG C^TTAAG
Asp718I 1136 GGTACC G^GTACC
AviII 1076 TGCGCA TGC^GCA
BalI 1437 TGGCCA TGG^CCA
BamHI 1544 GGATCC G^GATCC
BbeI 1554 GGCGCC GGCGC^C
BbvCI 1044 GCTGAGG CCTCAGC(-5/-2)
BfrI 745 CTTAAG C^TTAAG
BlpI 678 GCTGAGC GC^T[ACGT]AGC
BpmI 86 CTCCAG CTGGAG(16/14)
Bpu1102I 678 GCTGAGC GC^T[ACGT]AGC
BsaAI 883 TACGTG [CT]AC^GT[GA]
BsaHI 1554 GGCGCC G[GA]^CG[CT]C
BsmI 1883 GCATTC GAATGC(1/-1)
Bsp1407I 1591 TGTACA T^GTACA
BspMI 1354 GCAGGT ACCTGC(4/8)
BsrGI 1591 TGTACA T^GTACA
BssSI 103 CTCGTG CACGAG(-5/-1)
BstBI 1753 TTCGAA TT^CGAA
BstYI 1544 GGATCC [GA]^GATC[CT]
CelII 678 GCTGAGC GC^T[ACGT]AGC
DraI 1278 TTTAAA TTT^AAA
DraII 189 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 1121 CCATGG C^C[GA][CT]GG
EaeI 1437 TGGCCA [CT]^GGCC[GA]
EcoO109I 189 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRV 1322 GATATC GAT^ATC
FspI 1076 TGCGCA TGC^GCA
HaeII 1554 GGCGCC [GA]GCGC^[CT]
HgaI 714 GCGTC GACGC(5/10)
Hin1I 1554 GGCGCC G[GA]^CG[CT]C
HindIII 466 AAGCTT A^AGCTT
HpaI 643 GTTAAC GTT^AAC
KasI 1554 GGCGCC G^GCGCC
KpnI 1136 GGTACC GGTAC^C
MfeI 1706 CAATTG C^AATTG
MflI 1544 GGATCC [GA]^GATC[CT]
MluNI 1437 TGGCCA TGG^CCA
MscI 1437 TGGCCA TGG^CCA
MspA1I 1054 CAGCTG C[AC]G^C[GT]G
MunI 1706 CAATTG C^AATTG
NarI 1554 GGCGCC GG^CGCC
NcoI 1121 CCATGG C^CATGG
NspV 1753 TTCGAA TT^CGAA
PpuMI 189 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 1563 GACCACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1054 CAGCTG CAG^CTG
SapI 802 GAAGAGC GCTCTTC(1/4)
SfcI 520 CTACAG C^T[GA][CT]AG
SfoI 1554 GGCGCC GGC^GCC
SfuI 1753 TTCGAA TT^CGAA
SspBI 1591 TGTACA T^GTACA
XbaI 1939 TCTAGA T^CTAGA
XhoII 1544 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AseI 831 ATTAAT AT^TAAT
877 ATTAAT
AsnI 831 ATTAAT AT^TAAT
877 ATTAAT
BanI 1136 GGTACC G^G[CT][GA]CC
1554 GGCGCC
BseRI 1037 GAGGAG GAGGAG(10/8)
1460 GAGGAG
BsgI 1958 CTGCAC GTGCAG(16/14)
1816 GTGCAG
BsmFI 135 GTCCC GGGAC(10/14)
1947 GTCCC
BsrDI 674 CATTGC GCAATG(2/0)
1582 CATTGC
BstXI 4 CCATTGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1016 CCACTCACTTGG
Cac8I 943 GCCAGC GC[ACGT]^[ACGT]GC
1891 GCTTGC
EarI 109 GAAGAG CTCTTC(1/4)
802 GAAGAG
HincII 72 GTCAAC GT[CT]^[GA]AC
643 GTTAAC
HindII 72 GTCAAC GT[CT]^[GA]AC
643 GTTAAC
Ksp632I 109 GAAGAG CTCTTC(1/4)
802 GAAGAG
MaeII 641 ACGT A^CGT
884 ACGT
MslI 5 CATTGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1303 CACCTAAGTG
NspI 1079 GCATGT [GA]CATG^[CT]
1586 GCATGT
PleI 326 GAGTC GAGTC(4/5)
1945 GAGTC
PshBI 831 ATTAAT AT^TAAT
877 ATTAAT
SmlI 745 CTTAAG C^T[CT][GA]AG
763 CTTGAG
TfiI 525 GATTC G^A[AT]TC
1870 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AcsI AfaI AluI AlwI AlwNI
ApoI AspHI AvaII BanII BbsI
BbvI BfaI BmyI BpuAI BsaJI
BsiHKAI BsiYI BslI Bsp1286I BspHI
BsrI BssKI BstF5I BstNI CfoI
Cfr13I DdeI DpnII EcoRII EcoT14I
Fnu4HI FokI HaeIII HhaI HinP1I
HinfI HphI ItaI MaeI MaeIII
MboI MboII MnlI MseI MvaI
MwoI NdeII NlaIII NlaIV RcaI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I