Restriction map for
pFN21AE9647
##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AccIII AclI AgeI AhdI Aor51HI
ApaI AscI AspEI AspI AvrII
BbrPI BbvCI BclI BlnI BlpI
Bpu1102I BsaAI BsaI BseAI BsiEI
BsiWI BspDI BspEI BssHII CelII
ClaI CpoI EagI Eam1105I EclXI
Eco47III Eco52I EcoRV EcoT22I FbaI
FseI HpaI MluI MroI NcoI
NheI NotI NruI NsiI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SalI
SapI SbfI SfiI SnaBI SpeI
Sse8387I SspI StuI SwaI Tth111I
XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AcsI 2263 GAATTC [GA]^AATT[CT]
Alw44I 210 GTGCAC G^TGCAC
ApaLI 210 GTGCAC G^TGCAC
ApoI 2263 GAATTC [GA]^AATT[CT]
Asp700I 1888 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 2961 TGCGCA TGC^GCA
BamHI 833 GGATCC G^GATCC
BbeI 257 GGCGCC GGCGC^C
BcgI 1323 GCAGGAGTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaBI 2671 GATCTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 1840 GCATTC GAATGC(1/-1)
BspHI 4323 TCATGA T^CATGA
BssSI 1154 CTCGTG CACGAG(-5/-1)
BstBI 2330 TTCGAA TT^CGAA
BstEII 3213 GGTCACC G^GT[ACGT]ACC
BstPI 3213 GGTCACC G^GT[ACGT]ACC
DraI 1111 TTTAAA TTT^AAA
DrdI 3674 GACTAGGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3213 GGTCACC G^GT[ACGT]ACC
EcoRI 2263 GAATTC G^AATTC
FspI 2961 TGCGCA TGC^GCA
HgaI 727 GACGC GACGC(5/10)
HincII 748 GTCAAC GT[CT]^[GA]AC
HindII 748 GTCAAC GT[CT]^[GA]AC
HindIII 2203 AAGCTT A^AGCTT
KasI 257 GGCGCC G^GCGCC
KspI 205 CCGCGG CCGC^GG
MamI 2671 GATCTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 361 CAATTG C^AATTG
MunI 361 CAATTG C^AATTG
NaeI 3806 GCCGGC GCC^GGC
NarI 257 GGCGCC GG^CGCC
NdeI 2102 CATATG CA^TATG
NgoMIV 3806 GCCGGC G^CCGGC
NspV 2330 TTCGAA TT^CGAA
PshAI 2139 GACTGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1949 CTGCAG CTGCA^G
RcaI 4323 TCATGA T^CATGA
SacII 205 CCGCGG CCGC^GG
ScaI 1502 AGTACT AGT^ACT
SexAI 2295 ACCTGGT A^CC[AT]GGT
SfoI 257 GGCGCC GGC^GCC
SfuI 2330 TTCGAA TT^CGAA
SgrAI 3805 CGCCGGCG C[GA]^CCGG[CT]G
SphI 171 GCATGC GCATG^C
XmnI 1888 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AatII 958 GACGTC GACGT^C
1618 GACGTC
AccII 206 CGCG CG^CG
4229 CGCG
AflII 1834 CTTAAG C^TTAAG
3750 CTTAAG
AseI 2392 ATTAAT AT^TAAT
3274 ATTAAT
AsnI 2392 ATTAAT AT^TAAT
3274 ATTAAT
BciVI 4130 GGATAC GTATCC(6/5)
3861 GTATCC
BfrI 1834 CTTAAG C^TTAAG
3750 CTTAAG
BglII 1859 AGATCT A^GATCT
2618 AGATCT
BsaWI 836 TCCGGT [AT]^CCGG[AT]
1637 ACCGGA
BsmBI 3501 CGTCTC CGTCTC(1/5)
3894 CGTCTC
Bsp1407I 1217 TGTACA T^GTACA
3261 TGTACA
BspMI 128 ACCTGC ACCTGC(4/8)
3783 ACCTGC
BsrBI 1446 CCGCTC CCGCTC(-3/-3)
2383 GAGCGG
BsrFI 1005 GCCGGT [GA]^CCGG[CT]
3806 GCCGGC
BsrGI 1217 TGTACA T^GTACA
3261 TGTACA
Bst1107I 1770 GTATAC GTA^TAC
2548 GTATAC
BstAPI 1150 GCATCTCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3735 GCACCCAGTGC
BstUI 206 CGCG CG^CG
4229 CGCG
BstZ17I 1770 GTATAC GTA^TAC
2548 GTATAC
Cfr10I 1005 GCCGGT [GA]^CCGG[CT]
3806 GCCGGC
HaeII 257 GGCGCC [GA]GCGC^[CT]
413 AGCGCC
MvnI 206 CGCG CG^CG
4229 CGCG
PshBI 2392 ATTAAT AT^TAAT
3274 ATTAAT
PvuII 3982 CAGCTG CAG^CTG
4280 CAGCTG
SfcI 1949 CTGCAG C^T[GA][CT]AG
2050 CTACAG
SmaI 3563 CCCGGG CCC^GGG
3646 CCCGGG
SmlI 1834 CTTAAG C^T[CT][GA]AG
3750 CTTAAG
SspBI 1217 TGTACA T^GTACA
3261 TGTACA
XbaI 1302 TCTAGA T^CTAGA
2607 TCTAGA
XcmI 2483 CCAACCGAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3294 CCACATCATCCGTGG
XmaCI 3563 CCCGGG C^CCGGG
3646 CCCGGG
XmaI 3563 CCCGGG C^CCGGG
3646 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
Acc65I AccI AciI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
Asp718I AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspLU11I BsrDI BsrI BssKI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KpnI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII