Restriction map for
pFN21AE9611
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
Acc65I AccI AccII AclI AcsI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AvaI
AviII AvrII BalI BbeI BbrPI
BclI BcnI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsiEI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspMI BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII CfoI ClaI CpoI
DraI DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FbaI FseI
FspI HaeII HhaI HinP1I HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MluNI
MscI MseI MslI MspA1I MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SbfI SexAI SfiI
SfoI SfuI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI StyI
SwaI TaqI Tru9I TthHB8I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AatII 745 GACGTC GACGT^C
AccIII 443 TCCGGA T^CCGGA
AciI 198 GCGG CCGC(-3/-1)
AflIII 757 ACATGT A^C[GA][CT]GT
AlwNI 534 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 619 GGGCCC GGGCC^C
AspHI 137 GTGCTC G[AT]GC[AT]^C
AspI 421 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 672 GGATCC G^GATCC
BanI 200 GGCACC G^G[CT][GA]CC
BanII 619 GGGCCC G[GA]GC[CT]^C
BbsI 469 GTCTTC GAAGAC(2/6)
BbvCI 183 GCTGAGG CCTCAGC(-5/-2)
BcgI 830 CGAAGCCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 446 GGATAC GTATCC(6/5)
BpuAI 469 GTCTTC GAAGAC(2/6)
BsaWI 443 TCCGGA [AT]^CCGG[AT]
BseAI 443 TCCGGA T^CCGGA
BsgI 121 GTGCAG GTGCAG(16/14)
BsiHKAI 137 GTGCTC G[AT]GC[AT]^C
BsmFI 463 GTCCC GGGAC(10/14)
BsmI 552 GAATGC GAATGC(1/-1)
BspEI 443 TCCGGA T^CCGGA
BspHI 810 TCATGA T^CATGA
BspLU11I 757 ACATGT A^CATGT
BstF5I 516 CATCC GGATG(2/0)
FokI 516 CATCC GGATG(9/13)
HgaI 168 GCGTC GACGC(5/10)
MroI 443 TCCGGA T^CCGGA
PflFI 421 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 719 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 810 TCATGA T^CATGA
SapI 106 GAAGAGC GCTCTTC(1/4)
ScaI 379 AGTACT AGT^ACT
SgrAI 202 CACCGGCG C[GA]^CCGG[CT]G
SspI 500 AATATT AAT^ATT
StuI 333 AGGCCT AGG^CCT
Tsp509I 290 AATT ^AATT
Tth111I 421 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 245 CCAACCAAACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 167 GGCGTC G[GA]^CG[CT]C
745 GACGTC
AvaII 420 GGACC G^G[AT]CC
720 GGACC
BfaI 70 CTAG C^TAG
704 CTAG
BmyI 137 GTGCTC G[AGT]GC[ACT]^C
619 GGGCCC
BsaHI 167 GGCGTC G[GA]^CG[CT]C
745 GACGTC
BsaI 73 GAGACC GGTCTC(1/5)
894 GGTCTC
BseRI 359 GAGGAG GAGGAG(10/8)
793 GAGGAG
Bsp1286I 137 GTGCTC G[AGT]GC[ACT]^C
619 GGGCCC
BsrFI 203 ACCGGC [GA]^CCGG[CT]
584 ACCGGC
BstAPI 114 GCAGAGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
223 GCATGTATTGC
BstYI 88 AGATCC [GA]^GATC[CT]
672 GGATCC
Cfr10I 203 ACCGGC [GA]^CCGG[CT]
584 ACCGGC
DraII 619 GGGCCCT [GA]G^G[ACGT]CC[CT]
719 AGGACCT
EcoO109I 619 GGGCCCT [GA]G^G[ACGT]CC[CT]
719 AGGACCT
Hin1I 167 GGCGTC G[GA]^CG[CT]C
745 GACGTC
HphI 453 TCACC GGTGA(8/7)
749 TCACC
MaeI 70 CTAG C^TAG
704 CTAG
MaeII 21 ACGT A^CGT
746 ACGT
MflI 88 AGATCC [GA]^GATC[CT]
672 GGATCC
NspI 223 GCATGT [GA]CATG^[CT]
757 ACATGT
PflMI 249 CCAAACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
782 CCACTTCATGG
PleI 34 GAGTC GAGTC(4/5)
888 GAGTC
PstI 112 CTGCAG CTGCA^G
313 CTGCAG
SfcI 112 CTGCAG C^T[GA][CT]AG
313 CTGCAG
TfiI 153 GATTC G^A[AT]TC
406 GATTC
Van91I 249 CCAAACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
782 CCACTTCATGG
XhoII 88 AGATCC [GA]^GATC[CT]
672 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AfaI AluI AlwI BbvI BsaJI
BsiYI BslI BsmAI BsrI BssKI
BstNI Cac8I Cfr13I DdeI DpnII
EarI EcoRII Fnu4HI HaeIII HapII
HinfI HpaII ItaI Ksp632I MaeIII
MboI MboII MnlI MspI MvaI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI TseI
Tsp45I TspRI