Restriction map for
pFN21AE3804
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# NO CUT ENZYMES ( 165 enzymes) #
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AatII AccI AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AlwI AocI Aor51HI ApaI ApoI
AscI AseI AsnI Asp700I AspI
AvaI AviII AvrII BamHI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfrI BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BseAI BseRI BsgI BsiEI BsiWI
BsmAI BsmBI BsmI BsoBI Bsp1407I
BspEI BspHI BspLU11I BspMI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII CfoI Cfr10I CpoI
DraI DraII DraIII DrdI EagI
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoT22I
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI Ksp632I KspI
MamI MfeI MluI MroI MseI
MspA1I MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tru9I Tsp509I Tth111I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AhdI 274 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 85 GTGCAC G^TGCAC
AlwNI 588 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 85 GTGCAC G^TGCAC
AspEI 274 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 85 GTGCAC G[AT]GC[AT]^C
AvaII 21 GGACC G^G[AT]CC
BcnI 8 CCCGG CC^[GC]GG
BfaI 256 CTAG C^TAG
BglI 502 GCCAGTAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 179 AGATCT A^GATCT
BpmI 277 CTGGAG CTGGAG(16/14)
BsaWI 389 ACCGGA [AT]^CCGG[AT]
BsiHKAI 85 GTGCAC G[AT]GC[AT]^C
BspDI 376 ATCGAT AT^CGAT
BstAPI 587 GCAGATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 47 CCATTGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 179 AGATCT [GA]^GATC[CT]
ClaI 376 ATCGAT AT^CGAT
DdeI 534 CTTAG C^T[ACGT]AG
Eam1105I 274 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 298 GATATC GAT^ATC
FauI 306 GCGGG CCCGC(4/6)
MaeI 256 CTAG C^TAG
MboII 12 GAAGA GAAGA(8/7)
MflI 179 AGATCT [GA]^GATC[CT]
NciI 8 CCCGG CC^[GC]GG
PleI 280 GAGTC GAGTC(4/5)
SmlI 413 CTCAAG C^T[CT][GA]AG
XcmI 353 CCATGGTGTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 179 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 30 enzymes) #
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Acc65I 396 GGTACC G^GTACC
521 GGTACC
AfaI 397 GTAC GT^AC
522 GTAC
Asp718I 396 GGTACC G^GTACC
521 GGTACC
BalI 320 TGGCCA TGG^CCA
500 TGGCCA
BanI 396 GGTACC G^G[CT][GA]CC
521 GGTACC
BanII 237 GGGCTC G[GA]GC[CT]^C
509 GGGCTC
BbvI 248 GCTGC GCAGC(8/12)
593 GCTGC
BsmFI 185 GGGAC GGGAC(10/14)
331 GTCCC
BsrBI 210 CCGCTC CCGCTC(-3/-3)
202 GAGCGG
BstF5I 132 CATCC GGATG(2/0)
252 CATCC
Cfr13I 21 GGACC G^G[ACGT]CC
125 GGGCC
EaeI 320 TGGCCA [CT]^GGCC[GA]
500 TGGCCA
Fnu4HI 248 GCTGC GC^[ACGT]GC
593 GCTGC
FokI 132 CATCC GGATG(9/13)
252 CATCC
HapII 9 CCGG C^CGG
390 CCGG
HpaII 9 CCGG C^CGG
390 CCGG
ItaI 248 GCTGC GC^[ACGT]GC
593 GCTGC
KpnI 396 GGTACC GGTAC^C
521 GGTACC
MaeII 94 ACGT A^CGT
226 ACGT
MluNI 320 TGGCCA TGG^CCA
500 TGGCCA
MscI 320 TGGCCA TGG^CCA
500 TGGCCA
MspI 9 CCGG C^CGG
390 CCGG
RsaI 397 GTAC GT^AC
522 GTAC
Sau96I 21 GGACC G^G[ACGT]CC
125 GGGCC
TaqI 377 TCGA T^CGA
517 TCGA
TfiI 3 GAATC G^A[AT]TC
553 GAATC
TseI 248 GCTGC G^C[AT]GC
593 GCTGC
Tsp45I 158 GTGAC ^GT[GC]AC
268 GTCAC
TspRI 119 CACTG CA[GC]TG[ACGT][ACGT]^
456 CAGTG
TthHB8I 377 TCGA T^CGA
517 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AciI AluI BmyI BsaJI BsiYI
BslI Bsp1286I BsrI BssKI BstNI
Cac8I DpnII DsaI EcoRII EcoT14I
HaeIII HinfI HphI MaeIII MboI
MnlI MslI MvaI MwoI NcoI
NdeII NlaIII NlaIV Sau3AI ScrFI
SfaNI StyI