Restriction map for
pFN21AE9672
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# NO CUT ENZYMES ( 74 enzymes) #
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AatII AccI AccII AflII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI Asp700I AspEI AviII AvrII
BfrI BlnI BsaBI BsiEI BsiWI
BspLU11I BsrBI BsrFI BssHII Bst1107I
BstEII BstPI BstUI BstZ17I Cfr10I
CpoI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRV FseI FspI
HpaI KspI MamI MfeI MluI
MunI MvnI NaeI NcoI NdeI
NgoMIV NotI NruI PacI PflMI
PinAI PmeI PvuI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SgrAI SnaBI SpeI SphI Sse8387I
StuI Van91I XcmI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Acc65I 1501 GGTACC G^GTACC
AclI 1666 AACGTT AA^CGTT
AflIII 3431 ACACGT A^C[GA][CT]GT
AseI 921 ATTAAT AT^TAAT
AsnI 921 ATTAAT AT^TAAT
Asp718I 1501 GGTACC G^GTACC
AspI 4308 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 1368 GGCGCC GGCGC^C
BbrPI 4219 CACGTG CAC^GTG
BciVI 1445 GTATCC GTATCC(6/5)
BclI 2381 TGATCA T^GATCA
BglI 3826 GCCGGGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3478 AGATCT A^GATCT
BlpI 1825 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1825 GCTCAGC GC^T[ACGT]AGC
BsaAI 4219 CACGTG [CT]AC^GT[GA]
BspDI 3076 ATCGAT AT^CGAT
BspHI 768 TCATGA T^CATGA
BssSI 2800 CACGAG CACGAG(-5/-1)
BstBI 635 TTCGAA TT^CGAA
BstXI 2918 CCATCATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1825 GCTCAGC GC^T[ACGT]AGC
ClaI 3076 ATCGAT AT^CGAT
EcoRI 4065 GAATTC G^AATTC
EcoT22I 426 ATGCAT ATGCA^T
FbaI 2381 TGATCA T^GATCA
HindIII 3463 AAGCTT A^AGCTT
KasI 1368 GGCGCC G^GCGCC
KpnI 1501 GGTACC GGTAC^C
NarI 1368 GGCGCC GG^CGCC
NheI 3804 GCTAGC G^CTAGC
NsiI 426 ATGCAT ATGCA^T
NspV 635 TTCGAA TT^CGAA
PaeR7I 2845 CTCGAG C^TCGAG
PflFI 4308 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 4219 CACGTG CAC^GTG
PmlI 4219 CACGTG CAC^GTG
PshAI 1756 GACCAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 921 ATTAAT AT^TAAT
Psp1406I 1666 AACGTT AA^CGTT
RcaI 768 TCATGA T^CATGA
SfoI 1368 GGCGCC GGC^GCC
SfuI 635 TTCGAA TT^CGAA
SwaI 2051 ATTTAAAT ATTT^AAAT
Tth111I 4308 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2845 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 18 enzymes) #
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AccIII 1784 TCCGGA T^CCGGA
2981 TCCGGA
BalI 3903 TGGCCA TGG^CCA
3966 TGGCCA
BamHI 114 GGATCC G^GATCC
943 GGATCC
BcgI 3068 GCACTCTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4101 GCATCGGAGTCG
BsaI 2369 GGTCTC GGTCTC(1/5)
4418 GGTCTC
BsaWI 1784 TCCGGA [AT]^CCGG[AT]
2981 TCCGGA
BseAI 1784 TCCGGA T^CCGGA
2981 TCCGGA
BsmBI 2708 CGTCTC CGTCTC(1/5)
3282 GAGACG
Bsp1407I 318 TGTACA T^GTACA
714 TGTACA
BspEI 1784 TCCGGA T^CCGGA
2981 TCCGGA
BsrGI 318 TGTACA T^GTACA
714 TGTACA
DsaI 4082 CCGTGG C^C[GA][CT]GG
4303 CCACGG
MluNI 3903 TGGCCA TGG^CCA
3966 TGGCCA
MroI 1784 TCCGGA T^CCGGA
2981 TCCGGA
MscI 3903 TGGCCA TGG^CCA
3966 TGGCCA
NspI 2763 ACATGC [GA]CATG^[CT]
3813 ACATGC
SspBI 318 TGTACA T^GTACA
714 TGTACA
XbaI 2373 TCTAGA T^CTAGA
3309 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI AocI ApoI AspHI
AvaI AvaII BanI BanII BbsI
BbvCI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrDI BsrI BssKI BstAPI BstF5I
BstNI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DraIII DrdI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmaCI XmaI