Restriction map for
pFN21AE9451
##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AflIII AgeI AhdI AocI ApaI
AscI AseI AsnI Asp718I AspEI
AviII AvrII BanI BbeI BcgI
BfrI BglII BlnI BlpI Bpu1102I
BsaWI BseAI BsiWI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraIII DrdI
EagI Eam1105I EclXI Eco52I Eco81I
EcoO65I FseI FspI HpaI KasI
KpnI KspI MluI MroI NaeI
NarI NgoMIV NheI NotI NruI
NspV PacI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SspBI SspI SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
AatII 1191 GACGTC GACGT^C
AccII 1809 CGCG CG^CG
Alw44I 1049 GTGCAC G^TGCAC
AlwNI 417 CAGCGTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1475 AGCGCT AGC^GCT
ApaLI 1049 GTGCAC G^TGCAC
Asp700I 1905 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1538 CTCGAG C^[CT]CG[GA]G
BamHI 216 GGATCC G^GATCC
BbrPI 1164 CACGTG CAC^GTG
BbvCI 1259 CCTCAGC CCTCAGC(-5/-2)
BciVI 2126 GGATAC GTATCC(6/5)
BclI 2093 TGATCA T^GATCA
BcnI 373 CCGGG CC^[GC]GG
BfaI 494 CTAG C^TAG
BglI 737 GCCACCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1164 CACGTG [CT]AC^GT[GA]
BseRI 19 CTCCTC GAGGAG(10/8)
BsiEI 1103 CGACCG CG[GA][CT]^CG
BsmBI 1189 GAGACG CGTCTC(1/5)
BsoBI 1538 CTCGAG C^[CT]CG[GA]G
BstUI 1809 CGCG CG^CG
DraI 1467 TTTAAA TTT^AAA
DsaI 452 CCATGG C^C[GA][CT]GG
Eco47III 1475 AGCGCT AGC^GCT
EcoNI 2263 CCTGTGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1533 GAATTC G^AATTC
EcoRV 394 GATATC GAT^ATC
EcoT22I 2017 ATGCAT ATGCA^T
FauI 1810 GCGGG CCCGC(4/6)
FbaI 2093 TGATCA T^GATCA
HaeII 1475 AGCGCT [GA]GCGC^[CT]
HincII 445 GTCAAC GT[CT]^[GA]AC
HindII 445 GTCAAC GT[CT]^[GA]AC
HindIII 1712 AAGCTT A^AGCTT
MaeI 494 CTAG C^TAG
MfeI 1397 CAATTG C^AATTG
MunI 1397 CAATTG C^AATTG
MvnI 1809 CGCG CG^CG
NciI 373 CCGGG CC^[GC]GG
NcoI 452 CCATGG C^CATGG
NdeI 1034 CATATG CA^TATG
NsiI 2017 ATGCAT ATGCA^T
PaeR7I 1538 CTCGAG C^TCGAG
PleI 1541 GAGTC GAGTC(4/5)
PmaCI 1164 CACGTG CAC^GTG
PmlI 1164 CACGTG CAC^GTG
PpuMI 359 GGGTCCC [GA]G^G[AT]CC[CT]
SacI 1783 GAGCTC GAGCT^C
SapI 2214 GCTCTTC GCTCTTC(1/4)
SbfI 2307 CCTGCAGG CCTGCA^GG
SexAI 2182 ACCAGGT A^CC[AT]GGT
SphI 552 GCATGC GCATG^C
Sse8387I 2307 CCTGCAGG CCTGCA^GG
StuI 2201 AGGCCT AGG^CCT
TaqI 1539 TCGA T^CGA
Tsp45I 1345 GTGAC ^GT[GC]AC
TthHB8I 1539 TCGA T^CGA
XbaI 493 TCTAGA T^CTAGA
XcmI 1205 CCACAGTCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1538 CTCGAG C^TCGAG
XmnI 1905 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcyI 1191 GACGTC G[GA]^CG[CT]C
1773 GGCGTC
AspI 355 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
439 GACTGTGTC
BalI 621 TGGCCA TGG^CCA
1202 TGGCCA
BanII 867 GGGCTC G[GA]GC[CT]^C
1783 GAGCTC
BsaHI 1191 GACGTC G[GA]^CG[CT]C
1773 GGCGTC
BsaI 1420 GAGACC GGTCTC(1/5)
640 GGTCTC
BstAPI 416 GCAGCGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1753 GCAAATCTTGC
DraII 359 GGGTCCC [GA]G^G[ACGT]CC[CT]
2314 GGGGCCT
EcoO109I 359 GGGTCCC [GA]G^G[ACGT]CC[CT]
2314 GGGGCCT
HapII 373 CCGG C^CGG
1276 CCGG
HgaI 419 GCGTC GACGC(5/10)
1774 GCGTC
Hin1I 1191 GACGTC G[GA]^CG[CT]C
1773 GGCGTC
HpaII 373 CCGG C^CGG
1276 CCGG
MluNI 621 TGGCCA TGG^CCA
1202 TGGCCA
MscI 621 TGGCCA TGG^CCA
1202 TGGCCA
MspI 373 CCGG C^CGG
1276 CCGG
NspI 552 GCATGC [GA]CATG^[CT]
700 ACATGC
PflFI 355 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
439 GACTGTGTC
PflMI 754 CCAAGCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
783 CCAAGGAGTGG
PstI 1734 CTGCAG CTGCA^G
2308 CTGCAG
Tth111I 355 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
439 GACTGTGTC
Van91I 754 CCAAGCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
783 CCAAGGAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BbsI BbvI
BmyI BpmI BpuAI BsaBI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspMI BsrDI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII EaeI EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HhaI HinP1I
HinfI HphI ItaI Ksp632I MaeII
MaeIII MamI MboI MboII MflI
MnlI MseI MslI MspA1I MvaI
MwoI NdeII NlaIII NlaIV PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TfiI Tru9I
TseI Tsp509I TspRI XhoII