Restriction map for
pFN21AE9650
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# NO CUT ENZYMES ( 45 enzymes) #
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Acc65I AflII AscI Asp718I AviII
BciVI BfrI BsiWI BspDI BsrDI
BssHII BstBI ClaI CpoI EagI
EclXI Eco52I EcoRI EcoRV FseI
FspI HincII HindII HpaI KpnI
KspI MluI NotI NspV PacI
PflMI PmeI PshAI PvuI RsrII
SacII SalI SbfI SfiI SfuI
SgrAI SnaBI Sse8387I SwaI Van91I
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 4196 GACGTC GACGT^C
AclI 6022 AACGTT AA^CGTT
AgeI 2528 ACCGGT A^CCGGT
Alw44I 2571 GTGCAC G^TGCAC
Aor51HI 4217 AGCGCT AGC^GCT
ApaI 7311 GGGCCC GGGCC^C
ApaLI 2571 GTGCAC G^TGCAC
AseI 5529 ATTAAT AT^TAAT
AsnI 5529 ATTAAT AT^TAAT
AspI 534 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1020 GGATCC G^GATCC
BbeI 3248 GGCGCC GGCGC^C
BsmI 1602 GAATGC GAATGC(1/-1)
Bsp1407I 1664 TGTACA T^GTACA
BsrGI 1664 TGTACA T^GTACA
Bst1107I 183 GTATAC GTA^TAC
BstAPI 2184 GCACAACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 4264 GGTAACC G^GT[ACGT]ACC
BstPI 4264 GGTAACC G^GT[ACGT]ACC
BstZ17I 183 GTATAC GTA^TAC
DraIII 6316 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 4217 AGCGCT AGC^GCT
EcoO65I 4264 GGTAACC G^GT[ACGT]ACC
EcoT22I 5839 ATGCAT ATGCA^T
KasI 3248 GGCGCC G^GCGCC
MfeI 785 CAATTG C^AATTG
MunI 785 CAATTG C^AATTG
NaeI 4241 GCCGGC GCC^GGC
NarI 3248 GGCGCC GG^CGCC
NdeI 1217 CATATG CA^TATG
NgoMIV 4241 GCCGGC G^CCGGC
NruI 1356 TCGCGA TCG^CGA
NsiI 5839 ATGCAT ATGCA^T
PflFI 534 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2528 ACCGGT A^CCGGT
PshBI 5529 ATTAAT AT^TAAT
Psp1406I 6022 AACGTT AA^CGTT
SfoI 3248 GGCGCC GGC^GCC
SmaI 470 CCCGGG CCC^GGG
SpeI 7807 ACTAGT A^CTAGT
SphI 7547 GCATGC GCATG^C
SspBI 1664 TGTACA T^GTACA
SspI 1506 AATATT AAT^ATT
Tth111I 534 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 470 CCCGGG C^CCGGG
XmaI 470 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AflIII 5032 ACATGT A^C[GA][CT]GT
6876 ACATGT
AvrII 217 CCTAGG C^CTAGG
1467 CCTAGG
BbrPI 1230 CACGTG CAC^GTG
6316 CACGTG
BcgI 3280 CGAGCGGAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
8058 CGAACCCAGTGC
BglI 838 GCCTGGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
7003 GCCACCTCGGC
BlnI 217 CCTAGG C^CTAGG
1467 CCTAGG
BsaAI 1230 CACGTG [CT]AC^GT[GA]
6316 CACGTG
BsaBI 1249 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5005 GATGATCATC
BsiEI 2526 CGACCG CG[GA][CT]^CG
3135 CGACCG
BspHI 4799 TCATGA T^CATGA
7403 TCATGA
BspLU11I 5032 ACATGT A^CATGT
6876 ACATGT
BssSI 3237 CACGAG CACGAG(-5/-1)
6849 CACGAG
MamI 1249 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5005 GATGATCATC
NheI 3010 GCTAGC G^CTAGC
3609 GCTAGC
PmaCI 1230 CACGTG CAC^GTG
6316 CACGTG
PmlI 1230 CACGTG CAC^GTG
6316 CACGTG
RcaI 4799 TCATGA T^CATGA
7403 TCATGA
SapI 1006 GAAGAGC GCTCTTC(1/4)
1908 GCTCTTC
StuI 476 AGGCCT AGG^CCT
2586 AGGCCT
XbaI 2274 TCTAGA T^CTAGA
5188 TCTAGA
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# 3 OR MORE CUT ENZYMES ( 147 enzymes) #
#########################################
AccI AccII AccIII AciI AcsI
AcyI AfaI AhdI AluI AlwI
AlwNI AocI ApoI Asp700I AspEI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BclI
BcnI BfaI BglII BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BsaWI BseAI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspEI BspMI
BsrBI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DrdI
DsaI EaeI Eam1105I EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MroI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PaeR7I
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScaI ScrFI
SexAI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoI
XhoII XmnI