Restriction map for
pFN21AE9653
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# NO CUT ENZYMES ( 91 enzymes) #
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AatII Acc65I AccII AclI AcyI
AgeI Aor51HI AscI Asp718I AvaI
AviII BbeI BbrPI BciVI BcnI
BglI BsaAI BsaHI BsiEI BsiWI
BsmBI BsoBI BsrBI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I CpoI EagI EclXI
Eco47III Eco52I EcoO65I FauI FseI
FspI HaeII HgaI Hin1I HincII
HindII HpaI KasI KpnI KspI
MfeI MluI MunI MvnI NaeI
NarI NciI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SwaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccIII 1513 TCCGGA T^CCGGA
Alw44I 6697 GTGCAC G^TGCAC
AocI 3682 CCTAAGG CC^T[ACGT]AGG
ApaI 3153 GGGCCC GGGCC^C
ApaLI 6697 GTGCAC G^TGCAC
AseI 6778 ATTAAT AT^TAAT
AsnI 6778 ATTAAT AT^TAAT
AspHI 6697 GTGCAC G[AT]GC[AT]^C
AvrII 3024 CCTAGG C^CTAGG
BalI 2451 TGGCCA TGG^CCA
BanII 3153 GGGCCC G[GA]GC[CT]^C
BcgI 304 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 3024 CCTAGG C^CTAGG
BlpI 2275 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2275 GCTGAGC GC^T[ACGT]AGC
BseAI 1513 TCCGGA T^CCGGA
BsiHKAI 6697 GTGCAC G[AT]GC[AT]^C
BspDI 6142 ATCGAT AT^CGAT
BspEI 1513 TCCGGA T^CCGGA
BspHI 2214 TCATGA T^CATGA
BsrFI 5139 GCCGGT [GA]^CCGG[CT]
Bsu36I 3682 CCTAAGG CC^T[ACGT]AGG
CelII 2275 GCTGAGC GC^T[ACGT]AGC
CfoI 3886 GCGC GCG^C
Cfr10I 5139 GCCGGT [GA]^CCGG[CT]
ClaI 6142 ATCGAT AT^CGAT
DraIII 927 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 496 GACCAGACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 2451 TGGCCA [CT]^GGCC[GA]
Eco81I 3682 CCTAAGG CC^T[ACGT]AGG
EcoRV 2695 GATATC GAT^ATC
HhaI 3886 GCGC GCG^C
HinP1I 3886 GCGC G^CGC
MluNI 2451 TGGCCA TGG^CCA
MroI 1513 TCCGGA T^CCGGA
MscI 2451 TGGCCA TGG^CCA
PflMI 3076 CCAGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 6021 GACTAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 6778 ATTAAT AT^TAAT
RcaI 2214 TCATGA T^CATGA
Van91I 3076 CCAGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 4635 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 18 enzymes) #
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AccI 2095 GTCTAC GT^[AC][GT]AC
3859 GTAGAC
AflIII 2800 ACATGT A^C[GA][CT]GT
6643 ACATGT
AlwNI 1723 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
4706 CAGATCCTG
AspI 207 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2940 GACAGAGTC
BamHI 3967 GGATCC G^GATCC
4257 GGATCC
BanI 264 GGTGCC G^G[CT][GA]CC
2763 GGTGCC
BbvCI 315 CCTCAGC CCTCAGC(-5/-2)
4399 CCTCAGC
BclI 5460 TGATCA T^GATCA
6426 TGATCA
BsaWI 1513 TCCGGA [AT]^CCGG[AT]
1860 ACCGGA
BsmI 1110 GAATGC GAATGC(1/-1)
66 GCATTC
BspLU11I 2800 ACATGT A^CATGT
6643 ACATGT
BspMI 1068 ACCTGC ACCTGC(4/8)
5176 GCAGGT
EcoRI 2262 GAATTC G^AATTC
6682 GAATTC
EcoT22I 847 ATGCAT ATGCA^T
4867 ATGCAT
FbaI 5460 TGATCA T^GATCA
6426 TGATCA
NsiI 847 ATGCAT ATGCA^T
4867 ATGCAT
PflFI 207 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2940 GACAGAGTC
Tth111I 207 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2940 GACAGAGTC
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# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
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AciI AcsI AfaI AflII AhdI
AluI AlwI ApoI Asp700I AspEI
AvaII BbsI BbvI BfaI BfrI
BglII BmyI BpmI BpuAI BsaBI
BsaI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI Bsp1286I Bsp1407I
BsrDI BsrGI BsrI BssKI BstAPI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraI DraII DsaI
Eam1105I EarI EcoNI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NcoI NdeI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI SspI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI