Restriction map for
pFN21AE9640
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# NO CUT ENZYMES ( 80 enzymes) #
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AatII Acc65I AccI AccII AccIII
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspI AviII
AvrII BbeI BcgI BciVI BlnI
BseAI BsiEI BsiWI BsmBI BsmI
BspEI BspHI BssHII Bst1107I BstBI
BstUI BstZ17I CpoI DraI EagI
EclXI Eco47III Eco52I EcoT22I FseI
FspI HpaI KasI KpnI KspI
MfeI MluI MroI MunI MvnI
NaeI NarI NgoMIV NotI NruI
NsiI NspV PacI PflFI PmeI
PshBI PvuI RcaI RsrII SacII
SalI SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SwaI Tth111I XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 28 enzymes) #
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AcyI 3 GGCGTC G[GA]^CG[CT]C
AgeI 3494 ACCGGT A^CCGGT
BalI 3383 TGGCCA TGG^CCA
BbrPI 472 CACGTG CAC^GTG
BsaHI 3 GGCGTC G[GA]^CG[CT]C
BspDI 3265 ATCGAT AT^CGAT
BsrFI 3494 ACCGGT [GA]^CCGG[CT]
BssSI 812 CTCGTG CACGAG(-5/-1)
BstEII 4580 GGTCACC G^GT[ACGT]ACC
BstPI 4580 GGTCACC G^GT[ACGT]ACC
BstXI 2546 CCATTGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 3494 ACCGGT [GA]^CCGG[CT]
ClaI 3265 ATCGAT AT^CGAT
EcoO65I 4580 GGTCACC G^GT[ACGT]ACC
EcoRV 3628 GATATC GAT^ATC
Hin1I 3 GGCGTC G[GA]^CG[CT]C
HincII 4006 GTCAAC GT[CT]^[GA]AC
HindII 4006 GTCAAC GT[CT]^[GA]AC
MluNI 3383 TGGCCA TGG^CCA
MscI 3383 TGGCCA TGG^CCA
NheI 5190 GCTAGC G^CTAGC
PinAI 3494 ACCGGT A^CCGGT
PmaCI 472 CACGTG CAC^GTG
PmlI 472 CACGTG CAC^GTG
PshAI 2259 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 4215 AGTACT AGT^ACT
SexAI 2990 ACCAGGT A^CC[AT]GGT
SphI 4529 GCATGC GCATG^C
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# TWO CUT ENZYMES ( 34 enzymes) #
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AclI 4115 AACGTT AA^CGTT
5060 AACGTT
AflII 3022 CTTAAG C^TTAAG
3163 CTTAAG
AhdI 4048 GACGACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4060 GACAACCAGTC
AocI 4282 CCTCAGG CC^T[ACGT]AGG
4813 CCTGAGG
Asp700I 2433 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4338 GAAAGAGTTC
AspEI 4048 GACGACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4060 GACAACCAGTC
BamHI 1170 GGATCC G^GATCC
3906 GGATCC
BbvCI 4543 GCTGAGG CCTCAGC(-5/-2)
5446 GCTGAGG
BclI 1901 TGATCA T^GATCA
2540 TGATCA
BfrI 3022 CTTAAG C^TTAAG
3163 CTTAAG
BsaAI 472 CACGTG [CT]AC^GT[GA]
1458 TACGTG
BsaWI 3461 ACCGGA [AT]^CCGG[AT]
3494 ACCGGT
Bsp1407I 2253 TGTACA T^GTACA
4759 TGTACA
BsrGI 2253 TGTACA T^GTACA
4759 TGTACA
Bsu36I 4282 CCTCAGG CC^T[ACGT]AGG
4813 CCTGAGG
DraIII 1867 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
3320 CACCGGGTG
DrdI 2377 GACATGTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3504 GACCCGTGCGTC
EaeI 1591 CGGCCA [CT]^GGCC[GA]
3383 TGGCCA
Eam1105I 4048 GACGACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4060 GACAACCAGTC
Eco81I 4282 CCTCAGG CC^T[ACGT]AGG
4813 CCTGAGG
EcoRI 1327 GAATTC G^AATTC
5609 GAATTC
FbaI 1901 TGATCA T^GATCA
2540 TGATCA
HindIII 334 AAGCTT A^AGCTT
2056 AAGCTT
NcoI 5208 CCATGG C^CATGG
5423 CCATGG
NdeI 1321 CATATG CA^TATG
4352 CATATG
PaeR7I 903 CTCGAG C^TCGAG
3356 CTCGAG
Psp1406I 4115 AACGTT AA^CGTT
5060 AACGTT
PstI 1660 CTGCAG CTGCA^G
3142 CTGCAG
SacI 3455 GAGCTC GAGCT^C
3810 GAGCTC
SapI 357 GAAGAGC GCTCTTC(1/4)
5122 GAAGAGC
SspBI 2253 TGTACA T^GTACA
4759 TGTACA
XcmI 575 CCAAGATGATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5486 CCAAGGAGTCAATGG
XhoI 903 CTCGAG C^TCGAG
3356 CTCGAG
XmnI 2433 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4338 GAAAGAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BglI BglII BlpI
BmyI BpmI Bpu1102I BpuAI BsaBI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspLU11I BspMI BsrBI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII