Restriction map for
pFN21AB9997
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# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII AccIII AclI AgeI AhdI
Alw44I AocI ApaLI AscI AseI
AsnI AspEI AspI AviII AvrII
BamHI BbrPI BbvCI BciVI BclI
BglI BlnI BseAI BsiWI Bsp1407I
BspEI BsrGI BssHII Bst1107I BstBI
BstZ17I Bsu36I CpoI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoRI
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KspI MroI
NaeI NgoMIV NheI NotI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI SexAI SfiI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 2511 GGTACC G^GTACC
AccI 1656 GTCTAC GT^[AC][GT]AC
AcyI 35 GGCGCC G[GA]^CG[CT]C
ApaI 447 GGGCCC GGGCC^C
Asp700I 2928 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2511 GGTACC G^GTACC
BbeI 35 GGCGCC GGCGC^C
BbsI 1284 GAAGAC GAAGAC(2/6)
BglII 491 AGATCT A^GATCT
BlpI 9 GCTGAGC GC^T[ACGT]AGC
BpmI 2737 CTGGAG CTGGAG(16/14)
Bpu1102I 9 GCTGAGC GC^T[ACGT]AGC
BpuAI 1284 GAAGAC GAAGAC(2/6)
BsaAI 1737 CACGTA [CT]AC^GT[GA]
BsaBI 1167 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 35 GGCGCC G[GA]^CG[CT]C
BsiEI 915 CGACCG CG[GA][CT]^CG
BsmBI 1783 GAGACG CGTCTC(1/5)
BsmI 1794 GAATGC GAATGC(1/-1)
BspDI 322 ATCGAT AT^CGAT
BspHI 3332 TCATGA T^CATGA
BsrFI 3425 ACCGGC [GA]^CCGG[CT]
BssSI 1478 CTCGTG CACGAG(-5/-1)
BstAPI 43 GCACGACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 710 GGTCACC G^GT[ACGT]ACC
BstPI 710 GGTCACC G^GT[ACGT]ACC
CelII 9 GCTGAGC GC^T[ACGT]AGC
Cfr10I 3425 ACCGGC [GA]^CCGG[CT]
ClaI 322 ATCGAT AT^CGAT
DrdI 520 GACAGAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 710 GGTCACC G^GT[ACGT]ACC
EcoRV 319 GATATC GAT^ATC
Hin1I 35 GGCGCC G[GA]^CG[CT]C
KasI 35 GGCGCC G^GCGCC
KpnI 2511 GGTACC GGTAC^C
MamI 1167 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 692 CAATTG C^AATTG
MluI 1786 ACGCGT A^CGCGT
MunI 692 CAATTG C^AATTG
NarI 35 GGCGCC GG^CGCC
NcoI 2640 CCATGG C^CATGG
NdeI 1106 CATATG CA^TATG
NruI 3113 TCGCGA TCG^CGA
PflMI 309 CCAGAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 3332 TCATGA T^CATGA
SapI 463 GAAGAGC GCTCTTC(1/4)
ScaI 1023 AGTACT AGT^ACT
SfoI 35 GGCGCC GGC^GCC
SmaI 2807 CCCGGG CCC^GGG
Van91I 309 CCAGAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 2807 CCCGGG C^CCGGG
XmaI 2807 CCCGGG C^CCGGG
XmnI 2928 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AflII 1341 CTTAAG C^TTAAG
2071 CTTAAG
Aor51HI 13 AGCGCT AGC^GCT
2054 AGCGCT
AvaI 444 CTCGGG C^[CT]CG[GA]G
2807 CCCGGG
BfrI 1341 CTTAAG C^TTAAG
2071 CTTAAG
BsaI 3311 GAGACC GGTCTC(1/5)
160 GGTCTC
BsaWI 2551 ACCGGA [AT]^CCGG[AT]
2943 ACCGGA
BsoBI 444 CTCGGG C^[CT]CG[GA]G
2807 CCCGGG
BspLU11I 2696 ACATGT A^CATGT
2897 ACATGT
BstXI 1460 CCATTACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2501 CCAAGTTGTTGG
BstYI 491 AGATCT [GA]^GATC[CT]
3139 AGATCC
DraIII 1253 CACTAGGTG CAC[ACGT][ACGT][ACGT]^GTG
1291 CACCCTGTG
Eco47III 13 AGCGCT AGC^GCT
2054 AGCGCT
EcoT14I 2234 CCAAGG C^C[AT][AT]GG
2640 CCATGG
MflI 491 AGATCT [GA]^GATC[CT]
3139 AGATCC
PpuMI 141 AGGACCC [GA]G^G[AT]CC[CT]
1051 AGGTCCT
SmlI 1341 CTTAAG C^T[CT][GA]AG
2071 CTTAAG
SphI 1899 GCATGC GCATG^C
3446 GCATGC
StyI 2234 CCAAGG C^C[AT][AT]GG
2640 CCATGG
XcmI 2498 CCACCAAGTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2882 CCACCGAAACCGTGG
XhoII 491 AGATCT [GA]^GATC[CT]
3139 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaII BalI BanI BanII BbvI
BcgI BcnI BfaI BmyI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrBI
BsrDI BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI StuI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I