Restriction map for
pFN21AE3767
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# NO CUT ENZYMES ( 96 enzymes) #
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AccI AccIII AclI AhdI AocI
Aor51HI AscI AseI AsnI AspEI
AspI AvaI AvaII AvrII BamHI
BbvCI BcgI BciVI BcnI BfaI
BglII BlnI BsaI BseAI BsiEI
BsiWI BsmBI BsoBI BspEI BspHI
BspLU11I BspMI BssHII Bst1107I BstAPI
BstBI BstXI BstZ17I Bsu36I CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoRI
EcoT22I FseI HpaI KspI MaeI
MfeI MluI MroI MunI NaeI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PmeI PpuMI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI SspI
SwaI Tth111I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AatII 2134 GACGTC GACGT^C
Acc65I 2244 GGTACC G^GTACC
AflII 1920 CTTAAG C^TTAAG
AflIII 1010 ACGTGT A^C[GA][CT]GT
Alw44I 55 GTGCAC G^TGCAC
AlwI 1061 GATCC GGATC(4/5)
ApaI 207 GGGCCC GGGCC^C
ApaLI 55 GTGCAC G^TGCAC
Asp718I 2244 GGTACC G^GTACC
AviII 1887 TGCGCA TGC^GCA
BbeI 148 GGCGCC GGCGC^C
BbrPI 1009 CACGTG CAC^GTG
BfrI 1920 CTTAAG C^TTAAG
BglI 134 GCCGCACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1335 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1335 GCTGAGC GC^T[ACGT]AGC
BsaAI 1009 CACGTG [CT]AC^GT[GA]
BsaBI 282 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 461 TGTACA T^GTACA
BspDI 1252 ATCGAT AT^CGAT
BsrDI 1555 GCAATG GCAATG(2/0)
BsrGI 461 TGTACA T^GTACA
BssSI 13 CACGAG CACGAG(-5/-1)
BstEII 1115 GGTTACC G^GT[ACGT]ACC
BstPI 1115 GGTTACC G^GT[ACGT]ACC
BstYI 1060 AGATCC [GA]^GATC[CT]
CelII 1335 GCTGAGC GC^T[ACGT]AGC
ClaI 1252 ATCGAT AT^CGAT
EcoO65I 1115 GGTTACC G^GT[ACGT]ACC
EcoRV 1255 GATATC GAT^ATC
FauI 1366 GCGGG CCCGC(4/6)
FspI 1887 TGCGCA TGC^GCA
HaeII 148 GGCGCC [GA]GCGC^[CT]
HincII 2110 GTTGAC GT[CT]^[GA]AC
HindII 2110 GTTGAC GT[CT]^[GA]AC
KasI 148 GGCGCC G^GCGCC
KpnI 2244 GGTACC GGTAC^C
MamI 282 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1060 AGATCC [GA]^GATC[CT]
NarI 148 GGCGCC GG^CGCC
NcoI 1307 CCATGG C^CATGG
PflMI 2163 CCATACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1009 CACGTG CAC^GTG
PmlI 1009 CACGTG CAC^GTG
PshAI 1975 GACAAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 2205 GCTCTTC GCTCTTC(1/4)
SfoI 148 GGCGCC GGC^GCC
SspBI 461 TGTACA T^GTACA
StuI 1916 AGGCCT AGG^CCT
Van91I 2163 CCATACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1060 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 26 enzymes) #
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AgeI 1841 ACCGGT A^CCGGT
2192 ACCGGT
Asp700I 637 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1483 GAACGATTTC
BanI 148 GGCGCC G^G[CT][GA]CC
2244 GGTACC
BclI 1530 TGATCA T^GATCA
1739 TGATCA
BseRI 820 CTCCTC GAGGAG(10/8)
595 GAGGAG
BsmI 1362 GAATGC GAATGC(1/-1)
1086 GCATTC
BsrBI 908 CCGCTC CCGCTC(-3/-3)
166 GAGCGG
BsrFI 1841 ACCGGT [GA]^CCGG[CT]
2192 ACCGGT
Cfr10I 1841 ACCGGT [GA]^CCGG[CT]
2192 ACCGGT
DraII 206 AGGGCCC [GA]G^G[ACGT]CC[CT]
686 AGGCCCT
DsaI 1307 CCATGG C^C[GA][CT]GG
1757 CCGTGG
EcoO109I 206 AGGGCCC [GA]G^G[ACGT]CC[CT]
686 AGGCCCT
EcoT14I 1307 CCATGG C^C[AT][AT]GG
1832 CCAAGG
FbaI 1530 TGATCA T^GATCA
1739 TGATCA
HindIII 433 AAGCTT A^AGCTT
1684 AAGCTT
MaeII 1010 ACGT A^CGT
2135 ACGT
MslI 58 CACGCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1728 CACAGAAATG
PinAI 1841 ACCGGT A^CCGGT
2192 ACCGGT
SacI 961 GAGCTC GAGCT^C
1246 GAGCTC
SbfI 969 CCTGCAGG CCTGCA^GG
1812 CCTGCAGG
ScaI 479 AGTACT AGT^ACT
1850 AGTACT
SmlI 897 CTTGAG C^T[CT][GA]AG
1920 CTTAAG
Sse8387I 969 CCTGCAGG CCTGCA^GG
1812 CCTGCAGG
StyI 1307 CCATGG C^C[AT][AT]GG
1832 CCAAGG
XcmI 1988 CCATGTTCTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2003 CCAAGTGCCAGGTGG
XmnI 637 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1483 GAACGATTTC
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# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwNI ApoI AspHI BalI
BanII BbsI BbvI BmyI BpmI
BpuAI BsaHI BsaJI BsaWI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII EaeI EarI EcoNI EcoRII
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeIII MboI
MboII MluNI MnlI MscI MseI
MspA1I MspI MvaI MvnI MwoI
NdeII NlaIII NlaIV PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I