Restriction map for
pFN21AE3760
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# NO CUT ENZYMES ( 104 enzymes) #
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AatII Acc65I AclI AflIII AgeI
Alw44I AlwI AocI Aor51HI ApaLI
AscI AseI AsnI Asp718I AspI
AvrII BamHI BbsI BciVI BclI
BfaI BglII BlnI BpmI BpuAI
BsaBI BsaI BsiWI BsmBI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrDI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
ClaI DraI DraIII DrdI Eco47III
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI HindIII
HpaI HphI KpnI MaeI MaeIII
MamI MfeI MflI MluI MslI
MunI NcoI NdeI NheI NruI
NsiI NspV PacI PflFI PflMI
PinAI PmeI PshAI PshBI Psp1406I
RcaI SbfI ScaI SexAI SfuI
SnaBI SpeI Sse8387I SspBI SspI
StuI StyI SwaI Tsp45I TspRI
Tth111I Van91I XbaI XhoII
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccIII 1098 TCCGGA T^CCGGA
AcsI 352 AAATTC [GA]^AATT[CT]
AflII 301 CTTAAG C^TTAAG
AhdI 1351 GACTCGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 1040 GGGCCC GGGCC^C
ApoI 352 AAATTC [GA]^AATT[CT]
Asp700I 348 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1351 GACTCGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 548 TGCGCA TGC^GCA
BalI 65 TGGCCA TGG^CCA
BbrPI 498 CACGTG CAC^GTG
BcgI 1267 GCACCTCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 301 CTTAAG C^TTAAG
BlpI 1104 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1104 GCTCAGC GC^T[ACGT]AGC
BsaAI 498 CACGTG [CT]AC^GT[GA]
BsaWI 1098 TCCGGA [AT]^CCGG[AT]
BseAI 1098 TCCGGA T^CCGGA
BsmAI 1419 GTCTC GTCTC(1/5)
BspEI 1098 TCCGGA T^CCGGA
BsrI 522 CCAGT ACTGG(1/-1)
CelII 1104 GCTCAGC GC^T[ACGT]AGC
Eam1105I 1351 GACTCGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 879 CTCTTC CTCTTC(1/4)
FspI 548 TGCGCA TGC^GCA
Ksp632I 879 CTCTTC CTCTTC(1/4)
MluNI 65 TGGCCA TGG^CCA
MroI 1098 TCCGGA T^CCGGA
MscI 65 TGGCCA TGG^CCA
MseI 302 TTAA T^TAA
PaeR7I 820 CTCGAG C^TCGAG
PmaCI 498 CACGTG CAC^GTG
PmlI 498 CACGTG CAC^GTG
PstI 409 CTGCAG CTGCA^G
PvuI 384 CGATCG CGAT^CG
SapI 878 GCTCTTC GCTCTTC(1/4)
SfaNI 718 GATGC GCATC(5/9)
SfcI 409 CTGCAG C^T[GA][CT]AG
SfiI 924 GGCCTCCGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 1226 CGCCGGCG C[GA]^CCGG[CT]G
SphI 183 GCATGC GCATG^C
TfiI 152 GATTC G^A[AT]TC
Tru9I 302 TTAA T^TAA
Tsp509I 353 AATT ^AATT
XcmI 522 CCAGTTCGCGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 820 CTCGAG C^TCGAG
XmnI 348 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 3 GTCGAC GT^[AC][GT]AC
34 GTCGAC
AlwNI 252 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1201 CAGGGCCTG
AspHI 1102 GAGCTC G[AT]GC[AT]^C
1392 GAGCTC
BbvCI 771 CCTCAGC CCTCAGC(-5/-2)
1404 CCTCAGC
BsgI 403 CTGCAC GTGCAG(16/14)
760 CTGCAC
BsiHKAI 1102 GAGCTC G[AT]GC[AT]^C
1392 GAGCTC
BssSI 541 CACGAG CACGAG(-5/-1)
1068 CTCGTG
BstAPI 251 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
396 GCACCTCCTGC
DpnII 385 GATC ^GATC
444 GATC
HgaI 111 GACGC GACGC(5/10)
1330 GACGC
HincII 3 GTCGAC GT[CT]^[GA]AC
34 GTCGAC
HindII 3 GTCGAC GT[CT]^[GA]AC
34 GTCGAC
MaeII 499 ACGT A^CGT
850 ACGT
MboI 385 GATC ^GATC
444 GATC
MboII 880 TCTTC GAAGA(8/7)
1454 TCTTC
NdeII 385 GATC ^GATC
444 GATC
NotI 976 GCGGCCGC GC^GGCCGC
988 GCGGCCGC
PpuMI 692 GGGACCT [GA]G^G[AT]CC[CT]
1022 AGGACCT
SacI 1102 GAGCTC GAGCT^C
1392 GAGCTC
SalI 3 GTCGAC G^TCGAC
34 GTCGAC
Sau3AI 385 GATC ^GATC
444 GATC
SmaI 234 CCCGGG CCC^GGG
810 CCCGGG
SmlI 301 CTTAAG C^T[CT][GA]AG
820 CTCGAG
XmaCI 234 CCCGGG C^CCGGG
810 CCCGGG
XmaI 234 CCCGGG C^CCGGG
810 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AvaI AvaII BanI BanII BbeI
BbvI BcnI BglI BmyI BsaHI
BsaJI BseRI BsiEI BsiYI BslI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I CpoI
DdeI DraII DsaI EaeI EagI
EclXI Eco52I EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
ItaI KasI KspI MnlI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NgoMIV NlaIII NlaIV
NspI PleI PvuII RsaI RsrII
SacII Sau96I ScrFI SfoI TaqI
TseI TthHB8I