Restriction map for
pFN21AE3751
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# NO CUT ENZYMES ( 67 enzymes) #
##################################
Acc65I AclI AflII AgeI AhdI
Alw44I ApaLI AscI Asp700I Asp718I
AspEI AviII AvrII BclI BfaI
BfrI BlnI BsiWI BsmI Bsp1407I
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I CpoI DraIII Eam1105I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FspI HincII HindII HpaI KpnI
MaeI MfeI MluI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PinAI PmeI Psp1406I PvuI
RsrII SalI SapI SexAI SfuI
SgrAI SnaBI SpeI SspBI SwaI
XbaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AatII 3564 GACGTC GACGT^C
AflIII 3198 ACATGT A^C[GA][CT]GT
Aor51HI 1671 AGCGCT AGC^GCT
AseI 1398 ATTAAT AT^TAAT
AsnI 1398 ATTAAT AT^TAAT
BbeI 3117 GGCGCC GGCGC^C
BbrPI 85 CACGTG CAC^GTG
BcgI 1874 CGAGTCCGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4326 GGATAC GTATCC(6/5)
BlpI 4009 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 4009 GCTGAGC GC^T[ACGT]AGC
BsaAI 85 CACGTG [CT]AC^GT[GA]
BsaBI 1321 GATCGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 1870 GAGACG CGTCTC(1/5)
BspDI 2398 ATCGAT AT^CGAT
BspLU11I 3198 ACATGT A^CATGT
BsrDI 203 GCAATG GCAATG(2/0)
BssSI 494 CACGAG CACGAG(-5/-1)
CelII 4009 GCTGAGC GC^T[ACGT]AGC
ClaI 2398 ATCGAT AT^CGAT
DrdI 3660 GACGCCGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1671 AGCGCT AGC^GCT
FseI 4016 GGCCGGCC GGCCGG^CC
HindIII 1838 AAGCTT A^AGCTT
KasI 3117 GGCGCC G^GCGCC
KspI 1853 CCGCGG CCGC^GG
MamI 1321 GATCGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 3117 GGCGCC GG^CGCC
NcoI 1744 CCATGG C^CATGG
PmaCI 85 CACGTG CAC^GTG
PmlI 85 CACGTG CAC^GTG
PshBI 1398 ATTAAT AT^TAAT
SacII 1853 CCGCGG CCGC^GG
SbfI 3440 CCTGCAGG CCTGCA^GG
ScaI 638 AGTACT AGT^ACT
SfoI 3117 GGCGCC GGC^GCC
Sse8387I 3440 CCTGCAGG CCTGCA^GG
SspI 802 AATATT AAT^ATT
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# TWO CUT ENZYMES ( 18 enzymes) #
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AccI 274 GTCTAC GT^[AC][GT]AC
1126 GTCTAC
AccIII 839 TCCGGA T^CCGGA
1514 TCCGGA
AcsI 1006 AAATTC [GA]^AATT[CT]
1368 GAATTT
ApoI 1006 AAATTC [GA]^AATT[CT]
1368 GAATTT
BglII 92 AGATCT A^GATCT
3038 AGATCT
BseAI 839 TCCGGA T^CCGGA
1514 TCCGGA
BspEI 839 TCCGGA T^CCGGA
1514 TCCGGA
DraI 1273 TTTAAA TTT^AAA
1371 TTTAAA
MroI 839 TCCGGA T^CCGGA
1514 TCCGGA
PaeR7I 109 CTCGAG C^TCGAG
634 CTCGAG
PflMI 2757 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4291 CCAGACTCTGG
PshAI 2594 GACTGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3207 GACCAGTGTC
SmaI 2732 CCCGGG CCC^GGG
2826 CCCGGG
SphI 1064 GCATGC GCATG^C
4371 GCATGC
Van91I 2757 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4291 CCAGACTCTGG
XhoI 109 CTCGAG C^TCGAG
634 CTCGAG
XmaCI 2732 CCCGGG C^CCGGG
2826 CCCGGG
XmaI 2732 CCCGGG C^CCGGG
2826 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AspI AvaI AvaII BalI BamHI
BanI BanII BbsI BbvCI BbvI
BcnI BglI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspHI
BspMI BsrBI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI EarI EclXI Eco52I
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV NspI PflFI PleI PpuMI
PstI PvuII RcaI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfiI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I XcmI XhoII