Restriction map for
pFN21AE3752
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# NO CUT ENZYMES ( 193 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbsI
BbvI BcgI BciVI BclI BcnI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI BsmAI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstUI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FauI FbaI Fnu4HI FseI
FspI HaeII HapII HgaI Hin1I
HincII HindII HindIII HpaI HpaII
ItaI KasI KpnI Ksp632I KspI
MaeI MaeII MaeIII MfeI MluI
MluNI MroI MscI MseI MspA1I
MspI MunI MvnI MwoI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StyI
SwaI TfiI Tru9I TseI Tsp45I
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AcsI 226 AAATTC [GA]^AATT[CT]
AluI 384 AGCT AG^CT
ApoI 226 AAATTC [GA]^AATT[CT]
AspHI 446 GAGCAC G[AT]GC[AT]^C
AvaII 441 GGTCC G^G[AT]CC
BbvCI 15 CCTCAGC CCTCAGC(-5/-2)
BmyI 446 GAGCAC G[AGT]GC[ACT]^C
BpmI 306 CTGGAG CTGGAG(16/14)
BsaBI 196 GATGATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 292 GAGGAG GAGGAG(10/8)
BsiHKAI 446 GAGCAC G[AT]GC[AT]^C
Bsp1286I 446 GAGCAC G[AGT]GC[ACT]^C
BspHI 113 TCATGA T^CATGA
BstBI 229 TTCGAA TT^CGAA
BstF5I 182 GGATG GGATG(2/0)
BstXI 57 CCACCCATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 332 AGATCC [GA]^GATC[CT]
Cac8I 312 GCCAGC GC[ACGT]^[ACGT]GC
Cfr13I 441 GGTCC G^G[ACGT]CC
DsaI 154 CCGTGG C^C[GA][CT]GG
FokI 182 GGATG GGATG(9/13)
HphI 246 TCACC GGTGA(8/7)
MamI 196 GATGATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 208 TCTTC GAAGA(8/7)
MflI 332 AGATCC [GA]^GATC[CT]
NlaIV 440 GGGTCC GG[ACGT]^[ACGT]CC
NspV 229 TTCGAA TT^CGAA
RcaI 113 TCATGA T^CATGA
Sau96I 441 GGTCC G^G[ACGT]CC
SfaNI 183 GATGC GCATC(5/9)
SfuI 229 TTCGAA TT^CGAA
SmlI 386 CTCAAG C^T[CT][GA]AG
StuI 395 AGGCCT AGG^CCT
TaqI 230 TCGA T^CGA
Tsp509I 227 AATT ^AATT
TspRI 272 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 230 TCGA T^CGA
XhoII 332 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 25 enzymes) #
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AfaI 258 GTAC GT^AC
429 GTAC
AlwI 333 GATCC GGATC(4/5)
8 GGATC
BsaJI 29 CCCAGG C^C[ACGT][ACGT]GG
154 CCGTGG
BsiYI 29 CCCAGGAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
154 CCGTGGGAAGG
BslI 29 CCCAGGAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
154 CCGTGGGAAGG
BsrI 119 ACTGG ACTGG(1/-1)
305 ACTGG
BssKI 30 CCAGG ^CC[ACGT]GG
136 CCAGG
BstNI 30 CCAGG CC^[AT]GG
136 CCAGG
CfoI 128 GCGC GCG^C
466 GCGC
DdeI 16 CTCAG C^T[ACGT]AG
288 CTTAG
DpnII 9 GATC ^GATC
333 GATC
EcoRII 30 CCAGG ^CC[AT]GG
136 CCAGG
HaeIII 311 GGCC GG^CC
396 GGCC
HhaI 128 GCGC GCG^C
466 GCGC
HinP1I 128 GCGC G^CGC
466 GCGC
HinfI 23 GACTC G^A[ACGT]TC
150 GACTC
MboI 9 GATC ^GATC
333 GATC
MslI 215 CACCAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
247 CACCCGAATG
MvaI 30 CCAGG CC^[AT]GG
136 CCAGG
NdeII 9 GATC ^GATC
333 GATC
NlaIII 44 CATG CATG^
114 CATG
PleI 23 GACTC GAGTC(4/5)
150 GACTC
RsaI 258 GTAC GT^AC
429 GTAC
Sau3AI 9 GATC ^GATC
333 GATC
ScrFI 30 CCAGG CC^[ACGT]GG
136 CCAGG
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# 3 OR MORE CUT ENZYMES ( 2 enzymes) #
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BsmFI MnlI