Restriction map for
pFN21AB5958
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# NO CUT ENZYMES ( 142 enzymes) #
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AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BbeI BbrPI
BbsI BbvCI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BsrGI BssHII BstBI BstEII
BstPI BstUI Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraII
DraIII EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoT22I FauI FbaI FseI FspI
HaeII HhaI HinP1I HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tth111I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 441 GTATAC GT^[AC][GT]AC
AcyI 15 GGCGTC G[GA]^CG[CT]C
AfaI 136 GTAC GT^AC
AlwNI 1610 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 1165 CTCGGG C^[CT]CG[GA]G
BamHI 758 GGATCC G^GATCC
BanI 88 GGCACC G^G[CT][GA]CC
BcgI 511 CGATTGCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 5 CCGGG CC^[GC]GG
BglI 1584 GCCTTCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 15 GGCGTC G[GA]^CG[CT]C
BsmFI 633 GTCCC GGGAC(10/14)
BsoBI 1165 CTCGGG C^[CT]CG[GA]G
BspMI 779 ACCTGC ACCTGC(4/8)
Bst1107I 441 GTATAC GTA^TAC
BstAPI 865 GCATTTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1589 CCAGGCCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 441 GTATAC GTA^TAC
DrdI 592 GACAGAGTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1301 CCGTGG C^C[GA][CT]GG
EarI 1195 CTCTTC CTCTTC(1/4)
EcoRI 415 GAATTC G^AATTC
EcoRV 394 GATATC GAT^ATC
HapII 5 CCGG C^CGG
Hin1I 15 GGCGTC G[GA]^CG[CT]C
HpaII 5 CCGG C^CGG
Ksp632I 1195 CTCTTC CTCTTC(1/4)
MaeII 26 ACGT A^CGT
MseI 343 TTAA T^TAA
MspI 5 CCGG C^CGG
NciI 5 CCGGG CC^[GC]GG
PflMI 1025 CCAAAATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1092 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 385 CTGCAG CTGCA^G
RsaI 136 GTAC GT^AC
SacI 1192 GAGCTC GAGCT^C
SapI 1194 GCTCTTC GCTCTTC(1/4)
SphI 1181 GCATGC GCATG^C
StuI 1591 AGGCCT AGG^CCT
TaqI 1090 TCGA T^CGA
Tru9I 343 TTAA T^TAA
TthHB8I 1090 TCGA T^CGA
Van91I 1025 CCAAAATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 19 enzymes) #
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AspHI 1192 GAGCTC G[AT]GC[AT]^C
1351 GTGCTC
AvaII 1098 GGTCC G^G[AT]CC
1104 GGTCC
BalI 62 TGGCCA TGG^CCA
1082 TGGCCA
BseRI 103 GAGGAG GAGGAG(10/8)
1531 GAGGAG
BsiHKAI 1192 GAGCTC G[AT]GC[AT]^C
1351 GTGCTC
BsrDI 517 CATTGC GCAATG(2/0)
1247 GCAATG
BssSI 890 CACGAG CACGAG(-5/-1)
1528 CACGAG
BstYI 758 GGATCC [GA]^GATC[CT]
1175 AGATCC
HgaI 1365 GACGC GACGC(5/10)
16 GCGTC
HphI 1269 GGTGA GGTGA(8/7)
1355 TCACC
MflI 758 GGATCC [GA]^GATC[CT]
1175 AGATCC
MluNI 62 TGGCCA TGG^CCA
1082 TGGCCA
MscI 62 TGGCCA TGG^CCA
1082 TGGCCA
MspA1I 51 CAGCTG C[AC]G^C[GT]G
73 CAGCTG
NspI 539 ACATGC [GA]CATG^[CT]
1181 GCATGC
PvuII 51 CAGCTG CAG^CTG
73 CAGCTG
SfcI 98 CTATAG C^T[GA][CT]AG
385 CTGCAG
Tsp45I 1363 GTGAC ^GT[GC]AC
1386 GTCAC
XhoII 758 GGATCC [GA]^GATC[CT]
1175 AGATCC
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# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
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AciI AcsI AluI AlwI ApoI
BanII BbvI BfaI BmyI BpmI
BsaI BsaJI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII EaeI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HinfI ItaI MaeI MaeIII MboI
MboII MnlI MslI MvaI MwoI
NdeII NlaIII NlaIV PleI Sau3AI
Sau96I ScrFI SfaNI StyI TfiI
TseI Tsp509I TspRI XcmI