Restriction map for
pFN21AE9436
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# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII AccI AccII AclI AflII
AgeI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI AviII AvrII
BbeI BbvCI BcgI BcnI BfrI
BglII BlnI BsaBI BsgI BsiEI
BsiWI BsmBI BspDI BsrBI BsrFI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Cfr10I ClaI
CpoI DraIII DrdI DsaI EagI
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoRV FseI FspI HaeII
KasI KspI MamI MfeI MluI
MunI MvnI NaeI NarI NciI
NcoI NgoMIV NotI NruI NspV
PacI PaeR7I PflMI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI SspI SwaI Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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Acc65I 2232 GGTACC G^GTACC
AccIII 1967 TCCGGA T^CCGGA
AcyI 304 GGCGTC G[GA]^CG[CT]C
AflIII 2954 ACATGT A^C[GA][CT]GT
AhdI 1317 GACTATAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 2087 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2493 CCTCAGG CC^T[ACGT]AGG
Asp718I 2232 GGTACC G^GTACC
AspEI 1317 GACTATAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 478 GAGCTC G[AT]GC[AT]^C
AspI 1099 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 749 CACGTG CAC^GTG
BciVI 665 GGATAC GTATCC(6/5)
BlpI 838 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 838 GCTGAGC GC^T[ACGT]AGC
BsaAI 749 CACGTG [CT]AC^GT[GA]
BsaHI 304 GGCGTC G[GA]^CG[CT]C
BseAI 1967 TCCGGA T^CCGGA
BsiHKAI 478 GAGCTC G[AT]GC[AT]^C
Bsp1407I 559 TGTACA T^GTACA
BspEI 1967 TCCGGA T^CCGGA
BspHI 1503 TCATGA T^CATGA
BspLU11I 2954 ACATGT A^CATGT
BsrGI 559 TGTACA T^GTACA
BssSI 2046 CTCGTG CACGAG(-5/-1)
Bsu36I 2493 CCTCAGG CC^T[ACGT]AGG
CelII 838 GCTGAGC GC^T[ACGT]AGC
CfoI 1079 GCGC GCG^C
DraI 2742 TTTAAA TTT^AAA
DraII 815 AGGTCCC [GA]G^G[ACGT]CC[CT]
Eam1105I 1317 GACTATAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2493 CCTCAGG CC^T[ACGT]AGG
EcoO109I 815 AGGTCCC [GA]G^G[ACGT]CC[CT]
HgaI 305 GCGTC GACGC(5/10)
HhaI 1079 GCGC GCG^C
Hin1I 304 GGCGTC G[GA]^CG[CT]C
HinP1I 1079 GCGC G^CGC
KpnI 2232 GGTACC GGTAC^C
MroI 1967 TCCGGA T^CCGGA
NdeI 2152 CATATG CA^TATG
NheI 1515 GCTAGC G^CTAGC
PflFI 1099 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 749 CACGTG CAC^GTG
PmlI 749 CACGTG CAC^GTG
PpuMI 815 AGGTCCC [GA]G^G[AT]CC[CT]
PstI 715 CTGCAG CTGCA^G
RcaI 1503 TCATGA T^CATGA
SacI 478 GAGCTC GAGCT^C
SapI 2777 GCTCTTC GCTCTTC(1/4)
SbfI 714 CCTGCAGG CCTGCA^GG
SexAI 2027 ACCTGGT A^CC[AT]GGT
SmlI 1129 CTTGAG C^T[CT][GA]AG
Sse8387I 714 CCTGCAGG CCTGCA^GG
SspBI 559 TGTACA T^GTACA
StuI 2073 AGGCCT AGG^CCT
Tth111I 1099 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AvaI 794 CTCGGG C^[CT]CG[GA]G
1680 CCCGAG
BalI 24 TGGCCA TGG^CCA
2642 TGGCCA
BanII 478 GAGCTC G[GA]GC[CT]^C
2465 GAGCCC
BbsI 86 GTCTTC GAAGAC(2/6)
2032 GTCTTC
BglI 26 GCCATGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1249 GCCCCTCTGGC
BmyI 478 GAGCTC G[AGT]GC[ACT]^C
2465 GAGCCC
BpuAI 86 GTCTTC GAAGAC(2/6)
2032 GTCTTC
BsaI 2118 GGTCTC GGTCTC(1/5)
2895 GGTCTC
BsaWI 1967 TCCGGA [AT]^CCGG[AT]
2319 ACCGGA
BseRI 183 CTCCTC GAGGAG(10/8)
1742 GAGGAG
BsoBI 794 CTCGGG C^[CT]CG[GA]G
1680 CCCGAG
Bsp1286I 478 GAGCTC G[AGT]GC[ACT]^C
2465 GAGCCC
BstXI 744 CCATCCACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
779 CCATCCCAGTGG
EaeI 24 TGGCCA [CT]^GGCC[GA]
2642 TGGCCA
EcoT14I 1166 CCTTGG C^C[AT][AT]GG
2763 CCAAGG
EcoT22I 296 ATGCAT ATGCA^T
593 ATGCAT
HpaI 1695 GTTAAC GTT^AAC
2566 GTTAAC
MluNI 24 TGGCCA TGG^CCA
2642 TGGCCA
MscI 24 TGGCCA TGG^CCA
2642 TGGCCA
NsiI 296 ATGCAT ATGCA^T
593 ATGCAT
StyI 1166 CCTTGG C^C[AT][AT]GG
2763 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AvaII BamHI BanI
BbvI BclI BfaI BpmI BsaJI
BsiYI BslI BsmAI BsmFI BsmI
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII EarI EcoRII FauI FbaI
Fnu4HI FokI HaeIII HapII HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI