Restriction map for
pFN21AE0461
##################################
# NO CUT ENZYMES ( 172 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AvaII
AviII AvrII BamHI BanI BbeI
BbrPI BbsI BbvCI BclI BcnI
BglI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaJI BsaWI BseAI BsiEI
BsiWI BsmAI BsmBI BsoBI BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BssHII BssKI BssSI Bst1107I
BstAPI BstBI BstEII BstNI BstPI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI Cfr13I ClaI CpoI DraII
DraIII DrdI DsaI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRII
EcoT14I EcoT22I FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HpaI KasI KpnI Ksp632I KspI
MaeII MamI MluI MroI MslI
MvaI MvnI NarI NciI NcoI
NheI NlaIV NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
Sau96I SbfI ScaI ScrFI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI StyI SwaI Tsp45I Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AflII 303 CTTAAG C^TTAAG
AlwI 543 GGATC GGATC(4/5)
AspHI 257 GAGCTC G[AT]GC[AT]^C
BalI 168 TGGCCA TGG^CCA
BanII 257 GAGCTC G[GA]GC[CT]^C
BcgI 453 CGAAGCGAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 195 GGATAC GTATCC(6/5)
BfaI 281 CTAG C^TAG
BfrI 303 CTTAAG C^TTAAG
BmyI 257 GAGCTC G[AGT]GC[ACT]^C
BseRI 245 GAGGAG GAGGAG(10/8)
BsgI 8 CTGCAC GTGCAG(16/14)
BsiHKAI 257 GAGCTC G[AT]GC[AT]^C
BsiYI 589 CCGCAGGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 589 CCGCAGGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 53 GGGAC GGGAC(10/14)
BsmI 549 GAATGC GAATGC(1/-1)
Bsp1286I 257 GAGCTC G[AGT]GC[ACT]^C
Bsp1407I 567 TGTACA T^GTACA
BsrFI 599 GCCGGC [GA]^CCGG[CT]
BsrGI 567 TGTACA T^GTACA
BsrI 608 CCAGT ACTGG(1/-1)
BstF5I 531 GGATG GGATG(2/0)
BstYI 543 GGATCT [GA]^GATC[CT]
Cfr10I 599 GCCGGC [GA]^CCGG[CT]
DdeI 557 CTGAG C^T[ACGT]AG
DpnII 544 GATC ^GATC
DraI 34 TTTAAA TTT^AAA
EaeI 168 TGGCCA [CT]^GGCC[GA]
EcoRV 274 GATATC GAT^ATC
FokI 531 GGATG GGATG(9/13)
HapII 600 CCGG C^CGG
HincII 315 GTTGAC GT[CT]^[GA]AC
HindII 315 GTTGAC GT[CT]^[GA]AC
HindIII 537 AAGCTT A^AGCTT
HpaII 600 CCGG C^CGG
HphI 335 TCACC GGTGA(8/7)
MaeI 281 CTAG C^TAG
MboI 544 GATC ^GATC
MfeI 128 CAATTG C^AATTG
MflI 543 GGATCT [GA]^GATC[CT]
MluNI 168 TGGCCA TGG^CCA
MscI 168 TGGCCA TGG^CCA
MspA1I 309 CAGCTG C[AC]G^C[GT]G
MspI 600 CCGG C^CGG
MunI 128 CAATTG C^AATTG
NaeI 599 GCCGGC GCC^GGC
NdeI 404 CATATG CA^TATG
NdeII 544 GATC ^GATC
NgoMIV 599 GCCGGC G^CCGGC
NlaIII 57 CATG CATG^
PleI 559 GAGTC GAGTC(4/5)
PstI 184 CTGCAG CTGCA^G
PvuII 309 CAGCTG CAG^CTG
SacI 257 GAGCTC GAGCT^C
Sau3AI 544 GATC ^GATC
SspBI 567 TGTACA T^GTACA
TfiI 330 GAATC G^A[AT]TC
TspRI 609 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoII 543 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AciI 589 CCGC CCGC(-3/-1)
253 GCGG
AcsI 103 AAATTT [GA]^AATT[CT]
139 GAATTT
ApoI 103 AAATTT [GA]^AATT[CT]
139 GAATTT
Cac8I 77 GCTTGC GC[ACGT]^[ACGT]GC
599 GCCGGC
FauI 588 CCCGC CCCGC(4/6)
253 GCGGG
HaeIII 169 GGCC GG^CC
598 GGCC
HinfI 330 GAATC G^A[ACGT]TC
559 GAGTC
MaeIII 226 GTTAC ^GT[ACGT]AC
400 GTTAC
MseI 35 TTAA T^TAA
304 TTAA
MwoI 250 GCTGCGGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
635 GCTGTGGCTGC
SfaNI 322 GATGC GCATC(5/9)
487 GATGC
SfcI 184 CTGCAG C^T[GA][CT]AG
240 CTACAG
SmlI 303 CTTAAG C^T[CT][GA]AG
496 CTTGAG
TaqI 158 TCGA T^CGA
452 TCGA
Tru9I 35 TTAA T^TAA
304 TTAA
TthHB8I 158 TCGA T^CGA
452 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 10 enzymes) #
#########################################
AfaI AluI BbvI Fnu4HI ItaI
MboII MnlI RsaI TseI Tsp509I