Restriction map for
pFN21AE3996
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
Acc65I AccI AclI AcsI AflII
AgeI AhdI AlwNI AocI ApaI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BanII BbrPI BbvCI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsgI BsiWI BsmI BspHI
BspMI BsrDI BsrI BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstYI
BstZ17I Bsu36I CelII CpoI DraI
DraIII DrdI Eam1105I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HgaI HindIII HpaI KpnI
MaeI MamI MfeI MflI MluI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
TfiI Tsp509I Tth111I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AccIII 372 TCCGGA T^CCGGA
Alw44I 172 GTGCAC G^TGCAC
AlwI 539 GGATC GGATC(4/5)
Aor51HI 452 AGCGCT AGC^GCT
ApaLI 172 GTGCAC G^TGCAC
AspHI 172 GTGCAC G[AT]GC[AT]^C
AvaI 583 CCCGAG C^[CT]CG[GA]G
BalI 212 TGGCCA TGG^CCA
BbeI 479 GGCGCC GGCGC^C
BbsI 577 GTCTTC GAAGAC(2/6)
BmyI 172 GTGCAC G[AGT]GC[ACT]^C
BpuAI 577 GTCTTC GAAGAC(2/6)
BsaI 586 GAGACC GGTCTC(1/5)
BseAI 372 TCCGGA T^CCGGA
BsiHKAI 172 GTGCAC G[AT]GC[AT]^C
BsmBI 593 GAGACG CGTCTC(1/5)
BsmFI 81 GTCCC GGGAC(10/14)
BsoBI 583 CCCGAG C^[CT]CG[GA]G
Bsp1286I 172 GTGCAC G[AGT]GC[ACT]^C
Bsp1407I 197 TGTACA T^GTACA
BspDI 178 ATCGAT AT^CGAT
BspEI 372 TCCGGA T^CCGGA
BsrGI 197 TGTACA T^GTACA
BssHII 412 GCGCGC G^CGCGC
ClaI 178 ATCGAT AT^CGAT
DpnII 540 GATC ^GATC
EagI 19 CGGCCG C^GGCCG
EarI 253 CTCTTC CTCTTC(1/4)
EclXI 19 CGGCCG C^GGCCG
Eco47III 452 AGCGCT AGC^GCT
Eco52I 19 CGGCCG C^GGCCG
EcoNI 564 CCTCATCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 434 CCATGG C^C[AT][AT]GG
HincII 613 GTCAAC GT[CT]^[GA]AC
HindII 613 GTCAAC GT[CT]^[GA]AC
HinfI 235 GACTC G^A[ACGT]TC
KasI 479 GGCGCC G^GCGCC
Ksp632I 253 CTCTTC CTCTTC(1/4)
KspI 62 CCGCGG CCGC^GG
MboI 540 GATC ^GATC
MluNI 212 TGGCCA TGG^CCA
MroI 372 TCCGGA T^CCGGA
MscI 212 TGGCCA TGG^CCA
MseI 383 TTAA T^TAA
NarI 479 GGCGCC GG^CGCC
NcoI 434 CCATGG C^CATGG
NdeII 540 GATC ^GATC
PflMI 429 CCAGCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 235 GACTC GAGTC(4/5)
PshAI 388 GACTTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 62 CCGCGG CCGC^GG
Sau3AI 540 GATC ^GATC
SfaNI 131 GCATC GCATC(5/9)
SfoI 479 GGCGCC GGC^GCC
SspBI 197 TGTACA T^GTACA
StyI 434 CCATGG C^C[AT][AT]GG
Tru9I 383 TTAA T^TAA
Tsp45I 497 GTGAC ^GT[GC]AC
TspRI 264 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 429 CCAGCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AatII 354 GACGTC GACGT^C
595 GACGTC
AvaII 29 GGTCC G^G[AT]CC
633 GGACC
BanI 58 GGCACC G^G[CT][GA]CC
479 GGCGCC
BsaWI 372 TCCGGA [AT]^CCGG[AT]
589 ACCGGA
BseRI 406 GAGGAG GAGGAG(10/8)
571 GAGGAG
BsiEI 19 CGGCCG CG[GA][CT]^CG
100 CGGTCG
BstXI 203 CCAGCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
272 CCATCGTCCTGG
DdeI 340 CTGAG C^T[ACGT]AG
547 CTCAG
DsaI 62 CCGCGG C^C[GA][CT]GG
434 CCATGG
EaeI 19 CGGCCG [CT]^GGCC[GA]
212 TGGCCA
FauI 657 CCCGC CCCGC(4/6)
26 GCGGG
HaeII 452 AGCGCT [GA]GCGC^[CT]
479 GGCGCC
MaeIII 497 GTGAC ^GT[ACGT]AC
517 GTTAC
MboII 254 TCTTC GAAGA(8/7)
578 TCTTC
MslI 175 CACATCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
177 CATCGATGTG
NaeI 619 GCCGGC GCC^GGC
674 GCCGGC
NgoMIV 619 GCCGGC G^CCGGC
674 GCCGGC
PpuMI 28 GGGTCCT [GA]G^G[AT]CC[CT]
632 AGGACCC
TaqI 179 TCGA T^CGA
569 TCGA
TthHB8I 179 TCGA T^CGA
569 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI BbvI BcnI BpmI BsaHI
BsaJI BsiYI BslI BsmAI BspLU11I
BsrBI BsrFI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DraII EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HhaI Hin1I HinP1I
HpaII HphI ItaI MaeII MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NlaIII NlaIV NspI RsaI
Sau96I ScrFI TseI