Restriction map for
pFN21AE3991
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# NO CUT ENZYMES ( 190 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AgeI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspHI
AspI AvaI AvaII AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbvCI BbvI BcgI BclI
BcnI BfaI BfrI BglI BglII
BlnI BlpI BmyI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspMI BsrBI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII
BstPI BstUI BstXI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I ClaI
CpoI DraI DraII DraIII DsaI
EaeI EagI EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoT22I FauI FbaI Fnu4HI
FseI FspI HaeII HaeIII HapII
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HpaII HphI ItaI
KasI KpnI Ksp632I KspI MaeI
MaeII MamI MfeI MluI MluNI
MroI MscI MspI MunI MvnI
MwoI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NlaIV NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI Sau96I SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI TseI Tsp45I
Tth111I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AflIII 625 ACATGT A^C[GA][CT]GT
AhdI 30 GACTTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 754 GATCC GGATC(4/5)
AspEI 30 GACTTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BciVI 232 GGATAC GTATCC(6/5)
BsaJI 133 CCAAGG C^C[ACGT][ACGT]GG
BsiYI 430 CCATTAAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 430 CCATTAAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspLU11I 625 ACATGT A^CATGT
BsrDI 210 CATTGC GCAATG(2/0)
BssKI 384 CCTGG ^CC[ACGT]GG
Bst1107I 415 GTATAC GTA^TAC
BstNI 384 CCTGG CC^[AT]GG
BstYI 753 AGATCC [GA]^GATC[CT]
BstZ17I 415 GTATAC GTA^TAC
DrdI 468 GACAAGACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 30 GACTTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRII 384 CCTGG ^CC[AT]GG
EcoRV 116 GATATC GAT^ATC
EcoT14I 133 CCAAGG C^C[AT][AT]GG
HgaI 190 GACGC GACGC(5/10)
HinfI 795 GAATC G^A[ACGT]TC
MflI 753 AGATCC [GA]^GATC[CT]
MspA1I 685 CAGCTG C[AC]G^C[GT]G
MvaI 384 CCTGG CC^[AT]GG
NspI 625 ACATGT [GA]CATG^[CT]
PflMI 430 CCATTAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 685 CAGCTG CAG^CTG
ScaI 555 AGTACT AGT^ACT
ScrFI 384 CCTGG CC^[ACGT]GG
SfcI 94 CTACAG C^T[GA][CT]AG
SmlI 343 CTCAAG C^T[CT][GA]AG
SspI 831 AATATT AAT^ATT
StyI 133 CCAAGG C^C[AT][AT]GG
TfiI 795 GAATC G^A[AT]TC
Van91I 430 CCATTAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 620 CCAAAACATGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 753 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 11 enzymes) #
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AccI 415 GTATAC GT^[AC][GT]AC
857 GTCTAC
AluI 462 AGCT AG^CT
686 AGCT
Asp700I 334 GAATTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
830 GAATATTTTC
BbsI 348 GAAGAC GAAGAC(2/6)
698 GAAGAC
BpuAI 348 GAAGAC GAAGAC(2/6)
698 GAAGAC
MaeIII 125 GTTAC ^GT[ACGT]AC
380 GTAAC
MslI 389 CACTGATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
610 CATGAAGATG
SfaNI 616 GATGC GCATC(5/9)
440 GCATC
TaqI 260 TCGA T^CGA
568 TCGA
TthHB8I 260 TCGA T^CGA
568 TCGA
XmnI 334 GAATTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
830 GAATATTTTC
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# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AcsI AfaI ApoI BsrI BstF5I
DdeI DpnII FokI MboI MboII
MnlI MseI NdeII NlaIII RsaI
Sau3AI Tru9I Tsp509I TspRI