Restriction map for
pFN21AE3967
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# NO CUT ENZYMES ( 110 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AflII AgeI AhdI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI AviII BamHI
BbrPI BcgI BciVI BclI BfrI
BlpI Bpu1102I BsaAI BsaWI BseAI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrGI
BssHII BssSI Bst1107I BstEII BstPI
BstXI BstZ17I CelII ClaI CpoI
DraI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI HphI KpnI KspI MfeI
MluI MroI MunI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 69 enzymes) #
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AatII 868 GACGTC GACGT^C
AccII 513 CGCG CG^CG
AfaI 918 GTAC GT^AC
AflIII 686 ACACGT A^C[GA][CT]GT
AlwNI 951 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 948 CCTCAGG CC^T[ACGT]AGG
ApaI 959 GGGCCC GGGCC^C
Asp700I 1185 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 636 GTGCTC G[AT]GC[AT]^C
AvaI 43 CCCGGG C^[CT]CG[GA]G
AvrII 1087 CCTAGG C^CTAGG
BalI 1062 TGGCCA TGG^CCA
BanI 47 GGCGCC G^G[CT][GA]CC
BbeI 47 GGCGCC GGCGC^C
BbsI 255 GAAGAC GAAGAC(2/6)
BbvCI 819 GCTGAGG CCTCAGC(-5/-2)
BglI 925 GCCACGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1087 CCTAGG C^CTAGG
BpuAI 255 GAAGAC GAAGAC(2/6)
BsaBI 402 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 636 GTGCTC G[AT]GC[AT]^C
BsmBI 226 GAGACG CGTCTC(1/5)
BsoBI 43 CCCGGG C^[CT]CG[GA]G
BspMI 587 ACCTGC ACCTGC(4/8)
BsrFI 808 ACCGGC [GA]^CCGG[CT]
BstAPI 629 GCATGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1130 TTCGAA TT^CGAA
BstUI 513 CGCG CG^CG
Bsu36I 948 CCTCAGG CC^T[ACGT]AGG
CfoI 48 GCGC GCG^C
Cfr10I 808 ACCGGC [GA]^CCGG[CT]
DrdI 862 GACAGTGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 530 CTCTTC CTCTTC(1/4)
Eco81I 948 CCTCAGG CC^T[ACGT]AGG
FauI 850 CCCGC CCCGC(4/6)
HaeII 47 GGCGCC [GA]GCGC^[CT]
HhaI 48 GCGC GCG^C
HinP1I 48 GCGC G^CGC
HincII 844 GTTGAC GT[CT]^[GA]AC
HindII 844 GTTGAC GT[CT]^[GA]AC
KasI 47 GGCGCC G^GCGCC
Ksp632I 530 CTCTTC CTCTTC(1/4)
MamI 402 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1062 TGGCCA TGG^CCA
MscI 1062 TGGCCA TGG^CCA
MseI 691 TTAA T^TAA
MslI 454 CACCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 513 CGCG CG^CG
NarI 47 GGCGCC GG^CGCC
NcoI 854 CCATGG C^CATGG
NspI 629 GCATGC [GA]CATG^[CT]
NspV 1130 TTCGAA TT^CGAA
PflMI 1027 CCACATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 265 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 918 GTAC GT^AC
SfoI 47 GGCGCC GGC^GCC
SfuI 1130 TTCGAA TT^CGAA
SgrAI 807 CACCGGCG C[GA]^CCGG[CT]G
SmaI 43 CCCGGG CCC^GGG
SphI 629 GCATGC GCATG^C
TaqI 1131 TCGA T^CGA
Tru9I 691 TTAA T^TAA
Tsp509I 693 AATT ^AATT
TthHB8I 1131 TCGA T^CGA
Van91I 1027 CCACATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 106 CCAGATGGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 43 CCCGGG C^CCGGG
XmaI 43 CCCGGG C^CCGGG
XmnI 1185 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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AcyI 47 GGCGCC G[GA]^CG[CT]C
868 GACGTC
AlwI 156 GATCC GGATC(4/5)
953 GGATC
BanII 427 GGGCTC G[GA]GC[CT]^C
959 GGGCCC
BcnI 43 CCCGG CC^[GC]GG
44 CCGGG
BsaHI 47 GGCGCC G[GA]^CG[CT]C
868 GACGTC
BsaI 128 GGTCTC GGTCTC(1/5)
507 GGTCTC
BseRI 946 CTCCTC GAGGAG(10/8)
172 GAGGAG
BsgI 1014 CTGCAC GTGCAG(16/14)
418 GTGCAG
BsrDI 981 CATTGC GCAATG(2/0)
1041 CATTGC
BstF5I 183 CATCC GGATG(2/0)
858 GGATG
DraIII 485 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
1028 CACATGGTG
EcoT14I 854 CCATGG C^C[AT][AT]GG
1087 CCTAGG
FokI 183 CATCC GGATG(9/13)
858 GGATG
Hin1I 47 GGCGCC G[GA]^CG[CT]C
868 GACGTC
MboII 255 GAAGA GAAGA(8/7)
531 TCTTC
NciI 43 CCCGG CC^[GC]GG
44 CCGGG
PvuII 75 CAGCTG CAG^CTG
644 CAGCTG
SfaNI 143 GCATC GCATC(5/9)
536 GCATC
SmlI 37 CTCAAG C^T[CT][GA]AG
131 CTCAAG
StyI 854 CCATGG C^C[AT][AT]GG
1087 CCTAGG
TfiI 441 GAATC G^A[AT]TC
567 GATTC
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# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AluI AvaII BbvI BfaI
BglII BmyI BpmI BsaJI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII Fnu4HI HaeIII HapII
HgaI HinfI HpaII ItaI MaeI
MaeII MaeIII MboI MflI MnlI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV PleI PpuMI PstI
Sau3AI Sau96I ScrFI SfcI TseI
Tsp45I TspRI XhoII