Restriction map for
pFN21AE3954
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# NO CUT ENZYMES ( 95 enzymes) #
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Acc65I AccIII AclI AcsI AflII
AgeI Alw44I AocI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AvrII BamHI BbrPI BbvCI BciVI
BclI BfaI BfrI BlnI BlpI
Bpu1102I BsaBI BseAI BsmI BspEI
BspHI BspLU11I BsrDI BssSI BstBI
BstEII BstPI BstXI Bsu36I CelII
CpoI DraI DraII DraIII Eco81I
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HpaI KpnI MaeI
MamI MfeI MluI MroI MseI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
ScaI SexAI SfiI SfuI SmaI
SnaBI SpeI SspI SwaI Tru9I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AatII 139 GACGTC GACGT^C
AflIII 1412 ACGTGT A^C[GA][CT]GT
AlwI 1130 GGATC GGATC(4/5)
Asp700I 516 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 869 TGCGCA TGC^GCA
BalI 1199 TGGCCA TGG^CCA
BbsI 1186 GAAGAC GAAGAC(2/6)
BcgI 1727 GCAGGGGCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 380 AGATCT A^GATCT
BpuAI 1186 GAAGAC GAAGAC(2/6)
BsaAI 1299 CACGTA [CT]AC^GT[GA]
BsaI 909 GGTCTC GGTCTC(1/5)
BsaWI 1403 ACCGGA [AT]^CCGG[AT]
BsiWI 1301 CGTACG C^GTACG
Bsp1407I 1337 TGTACA T^GTACA
BspDI 499 ATCGAT AT^CGAT
BsrGI 1337 TGTACA T^GTACA
BsrI 1572 CCAGT ACTGG(1/-1)
BssHII 1444 GCGCGC G^CGCGC
Bst1107I 64 GTATAC GTA^TAC
BstAPI 162 GCAGCGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 380 AGATCT [GA]^GATC[CT]
BstZ17I 64 GTATAC GTA^TAC
ClaI 499 ATCGAT AT^CGAT
DrdI 21 GACGCGAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 869 TGCGCA TGC^GCA
HincII 1735 GTCGAC GT[CT]^[GA]AC
HindII 1735 GTCGAC GT[CT]^[GA]AC
HindIII 242 AAGCTT A^AGCTT
KspI 123 CCGCGG CCGC^GG
MflI 380 AGATCT [GA]^GATC[CT]
MluNI 1199 TGGCCA TGG^CCA
MscI 1199 TGGCCA TGG^CCA
NcoI 1520 CCATGG C^CATGG
PflMI 687 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 1662 CAGCTG CAG^CTG
SacI 928 GAGCTC GAGCT^C
SacII 123 CCGCGG CCGC^GG
SalI 1735 GTCGAC G^TCGAC
SapI 129 GCTCTTC GCTCTTC(1/4)
SgrAI 1047 CGCCGGCG C[GA]^CCGG[CT]G
SspBI 1337 TGTACA T^GTACA
StuI 1550 AGGCCT AGG^CCT
TfiI 52 GAATC G^A[AT]TC
Tsp509I 1365 AATT ^AATT
Van91I 687 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 380 AGATCT [GA]^GATC[CT]
XmnI 516 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 30 enzymes) #
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AhdI 1245 GACGTACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1264 GACTCCTGGTC
Aor51HI 1227 AGCGCT AGC^GCT
1526 AGCGCT
AspEI 1245 GACGTACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1264 GACTCCTGGTC
AspI 139 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
349 GACTACGTC
AvaI 664 CCCGAG C^[CT]CG[GA]G
1344 CTCGGG
BbeI 271 GGCGCC GGCGC^C
1587 GGCGCC
BcnI 998 CCCGG CC^[GC]GG
1839 CCGGG
BglI 993 GCCCGCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1307 GCCACGCGGGC
BseRI 802 GAGGAG GAGGAG(10/8)
1420 GAGGAG
BsmFI 1067 GGGAC GGGAC(10/14)
1253 GTCCC
BsoBI 664 CCCGAG C^[CT]CG[GA]G
1344 CTCGGG
DdeI 589 CTGAG C^T[ACGT]AG
931 CTCAG
EagI 752 CGGCCG C^GGCCG
1209 CGGCCG
Eam1105I 1245 GACGTACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1264 GACTCCTGGTC
EarI 130 CTCTTC CTCTTC(1/4)
1233 CTCTTC
EclXI 752 CGGCCG C^GGCCG
1209 CGGCCG
Eco47III 1227 AGCGCT AGC^GCT
1526 AGCGCT
Eco52I 752 CGGCCG C^GGCCG
1209 CGGCCG
KasI 271 GGCGCC G^GCGCC
1587 GGCGCC
Ksp632I 130 CTCTTC CTCTTC(1/4)
1233 CTCTTC
MaeIII 935 GTGAC ^GT[ACGT]AC
1475 GTGAC
NarI 271 GGCGCC GG^CGCC
1587 GGCGCC
NciI 998 CCCGG CC^[GC]GG
1839 CCGGG
PflFI 139 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
349 GACTACGTC
SbfI 450 CCTGCAGG CCTGCA^GG
630 CCTGCAGG
SfoI 271 GGCGCC GGC^GCC
1587 GGCGCC
SphI 233 GCATGC GCATG^C
1599 GCATGC
Sse8387I 450 CCTGCAGG CCTGCA^GG
630 CCTGCAGG
Tsp45I 935 GTGAC ^GT[GC]AC
1475 GTGAC
Tth111I 139 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
349 GACTACGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwNI AspHI AvaII BanI
BanII BbvI BmyI BpmI BsaHI
BsaJI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI Bsp1286I BspMI
BsrBI BsrFI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DpnII DsaI EaeI EcoNI EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeII MboI MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NdeII NgoMIV NlaIII NlaIV
NspI PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TseI TspRI TthHB8I