Restriction map for
pFN21AE3945
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaII AviII AvrII BanI BbeI
BbrPI BbvCI BcgI BclI BcnI
BfrI BglI BlnI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsiEI BsiWI BsmBI BsmFI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstZ17I Bsu36I CfoI Cfr10I Cfr13I
CpoI DraII DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO109I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HpaI HpaII KasI KpnI
KspI MamI MluI MroI MspI
NaeI NarI NciI NcoI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacII SalI SapI
Sau96I SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I StuI StyI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccII 810 CGCG CG^CG
AflIII 1786 ACACGT A^C[GA][CT]GT
AlwNI 529 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 1596 GAGCTC G[AT]GC[AT]^C
AvaI 441 CCCGAG C^[CT]CG[GA]G
BamHI 237 GGATCC G^GATCC
BanII 1596 GAGCTC G[GA]GC[CT]^C
BciVI 662 GTATCC GTATCC(6/5)
BlpI 988 GCTGAGC GC^T[ACGT]AGC
BmyI 1596 GAGCTC G[AGT]GC[ACT]^C
Bpu1102I 988 GCTGAGC GC^T[ACGT]AGC
BsgI 980 CTGCAC GTGCAG(16/14)
BsiHKAI 1596 GAGCTC G[AT]GC[AT]^C
BsmI 967 GAATGC GAATGC(1/-1)
BsoBI 441 CCCGAG C^[CT]CG[GA]G
Bsp1286I 1596 GAGCTC G[AGT]GC[ACT]^C
BspDI 97 ATCGAT AT^CGAT
BspMI 115 GCAGGT ACCTGC(4/8)
BstEII 868 GGTTACC G^GT[ACGT]ACC
BstPI 868 GGTTACC G^GT[ACGT]ACC
BstUI 810 CGCG CG^CG
BstXI 1601 CCATCAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 988 GCTGAGC GC^T[ACGT]AGC
ClaI 97 ATCGAT AT^CGAT
DraI 1791 TTTAAA TTT^AAA
DsaI 956 CCACGG C^C[GA][CT]GG
EcoNI 51 CCTTCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 868 GGTTACC G^GT[ACGT]ACC
FauI 12 GCGGG CCCGC(4/6)
HindIII 1538 AAGCTT A^AGCTT
MfeI 1637 CAATTG C^AATTG
MslI 1124 CATCTGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 10 CAGCGG C[AC]G^C[GT]G
MunI 1637 CAATTG C^AATTG
MvnI 810 CGCG CG^CG
NdeI 1701 CATATG CA^TATG
PshAI 121 GACAAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1596 GAGCTC GAGCT^C
ScaI 179 AGTACT AGT^ACT
SfcI 1090 CTACAG C^T[GA][CT]AG
SspI 689 AATATT AAT^ATT
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Asp700I 87 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1004 GAAATCATTC
BalI 326 TGGCCA TGG^CCA
1314 TGGCCA
BbsI 813 GAAGAC GAAGAC(2/6)
128 GTCTTC
BbvI 937 GCAGC GCAGC(8/12)
1270 GCTGC
BglII 72 AGATCT A^GATCT
1428 AGATCT
BpmI 742 CTCCAG CTGGAG(16/14)
1663 CTGGAG
BpuAI 813 GAAGAC GAAGAC(2/6)
128 GTCTTC
BsaJI 886 CCCAGG C^C[ACGT][ACGT]GG
956 CCACGG
Bsp1407I 486 TGTACA T^GTACA
1381 TGTACA
BsrGI 486 TGTACA T^GTACA
1381 TGTACA
EaeI 326 TGGCCA [CT]^GGCC[GA]
1314 TGGCCA
HaeIII 327 GGCC GG^CC
1315 GGCC
MaeII 1755 ACGT A^CGT
1788 ACGT
MluNI 326 TGGCCA TGG^CCA
1314 TGGCCA
MscI 326 TGGCCA TGG^CCA
1314 TGGCCA
NlaIV 237 GGATCC GG[ACGT]^[ACGT]CC
1434 GGCTCC
PleI 446 GACTC GAGTC(4/5)
1666 GAGTC
SfaNI 769 GATGC GCATC(5/9)
1123 GCATC
SspBI 486 TGTACA T^GTACA
1381 TGTACA
TaqI 98 TCGA T^CGA
1112 TCGA
TseI 937 GCAGC G^C[AT]GC
1270 GCTGC
Tsp45I 119 GTGAC ^GT[GC]AC
1507 GTCAC
TthHB8I 98 TCGA T^CGA
1112 TCGA
XmnI 87 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1004 GAAATCATTC
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# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BfaI BsiYI BslI BsmAI
BsrI BssKI BstF5I BstNI BstYI
Cac8I DdeI DpnII EarI EcoRII
Fnu4HI FokI HinfI HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MvaI MwoI
NdeII NlaIII RsaI Sau3AI ScrFI
TfiI Tru9I Tsp509I TspRI XhoII