Restriction map for
pFN21AE3927
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# NO CUT ENZYMES ( 149 enzymes) #
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AatII Acc65I AccI AccIII AciI
AcyI AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BamHI BanI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Bsu36I Cac8I CelII Cfr10I
ClaI CpoI DraII DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FauI FseI
FspI HaeII HgaI Hin1I HindIII
KasI KpnI Ksp632I KspI MamI
MfeI MluI MroI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NlaIV NotI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI PstI
PvuI RcaI RsrII SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SwaI Tth111I
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccII 31 CGCG CG^CG
AclI 144 AACGTT AA^CGTT
AcsI 224 AAATTT [GA]^AATT[CT]
AfaI 688 GTAC GT^AC
AflII 985 CTTAAG C^TTAAG
AflIII 171 ACATGT A^C[GA][CT]GT
ApoI 224 AAATTT [GA]^AATT[CT]
Asp700I 487 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 1210 GGACC G^G[AT]CC
BalI 1188 TGGCCA TGG^CCA
BclI 579 TGATCA T^GATCA
BcnI 862 CCGGG CC^[GC]GG
BfrI 985 CTTAAG C^TTAAG
BpmI 587 CTCCAG CTGGAG(16/14)
BsiYI 499 CCAAATACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 499 CCAAATACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 573 GTCCC GGGAC(10/14)
BsmI 193 GAATGC GAATGC(1/-1)
BspLU11I 171 ACATGT A^CATGT
BstAPI 937 GCATACCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 31 CGCG CG^CG
CfoI 886 GCGC GCG^C
DraI 228 TTTAAA TTT^AAA
DsaI 716 CCGTGG C^C[GA][CT]GG
EaeI 1188 TGGCCA [CT]^GGCC[GA]
EcoT14I 1053 CCTTGG C^C[AT][AT]GG
FbaI 579 TGATCA T^GATCA
HapII 862 CCGG C^CGG
HhaI 886 GCGC GCG^C
HinP1I 886 GCGC G^CGC
HincII 1176 GTTAAC GT[CT]^[GA]AC
HindII 1176 GTTAAC GT[CT]^[GA]AC
HpaI 1176 GTTAAC GTT^AAC
HpaII 862 CCGG C^CGG
HphI 1266 GGTGA GGTGA(8/7)
MluNI 1188 TGGCCA TGG^CCA
MscI 1188 TGGCCA TGG^CCA
MslI 889 CATACTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 862 CCGG C^CGG
MvnI 31 CGCG CG^CG
NciI 862 CCGGG CC^[GC]GG
NruI 30 TCGCGA TCG^CGA
NspI 171 ACATGT [GA]CATG^[CT]
PflMI 499 CCAAATACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 34 GAGTC GAGTC(4/5)
Psp1406I 144 AACGTT AA^CGTT
RsaI 688 GTAC GT^AC
ScaI 687 AGTACT AGT^ACT
SfcI 841 CTACAG C^T[GA][CT]AG
StuI 876 AGGCCT AGG^CCT
StyI 1053 CCTTGG C^C[AT][AT]GG
TaqI 657 TCGA T^CGA
TfiI 240 GAATC G^A[AT]TC
TthHB8I 657 TCGA T^CGA
Van91I 499 CCAAATACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 487 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
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AlwI 567 GATCC GGATC(4/5)
859 GATCC
AspHI 163 GAGCTC G[AT]GC[AT]^C
670 GAGCTC
BanII 163 GAGCTC G[GA]GC[CT]^C
670 GAGCTC
BmyI 163 GAGCTC G[AGT]GC[ACT]^C
670 GAGCTC
BsiHKAI 163 GAGCTC G[AT]GC[AT]^C
670 GAGCTC
Bsp1286I 163 GAGCTC G[AGT]GC[ACT]^C
670 GAGCTC
BsrI 505 ACTGG ACTGG(1/-1)
828 CCAGT
BstNI 415 CCTGG CC^[AT]GG
1182 CCTGG
BstYI 566 AGATCC [GA]^GATC[CT]
858 AGATCC
Cfr13I 1057 GGGCC G^G[ACGT]CC
1210 GGACC
EcoRII 415 CCTGG ^CC[AT]GG
1182 CCTGG
HinfI 34 GAGTC G^A[ACGT]TC
240 GAATC
MflI 566 AGATCC [GA]^GATC[CT]
858 AGATCC
MvaI 415 CCTGG CC^[AT]GG
1182 CCTGG
MwoI 937 GCATACCTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1123 GCTCCAACAGC
SacI 163 GAGCTC GAGCT^C
670 GAGCTC
Sau96I 1057 GGGCC G^G[ACGT]CC
1210 GGACC
SfaNI 509 GATGC GCATC(5/9)
990 GCATC
SmlI 38 CTCAAG C^T[CT][GA]AG
985 CTTAAG
Tsp45I 454 GTGAC ^GT[GC]AC
1162 GTGAC
XbaI 369 TCTAGA T^CTAGA
1236 TCTAGA
XhoII 566 AGATCC [GA]^GATC[CT]
858 AGATCC
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# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AluI BbvI BfaI BsaJI BsmAI
BssKI BstF5I DdeI DpnII Fnu4HI
FokI HaeIII ItaI MaeI MaeII
MaeIII MboI MboII MnlI MseI
MspA1I NdeII NlaIII PvuII Sau3AI
ScrFI SspI Tru9I TseI Tsp509I
TspRI