Restriction map for
pFN21AE3657
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# NO CUT ENZYMES ( 99 enzymes) #
##################################
Acc65I AccI AclI AflII AgeI
AocI Aor51HI AscI AseI AsnI
Asp700I Asp718I AviII AvrII BamHI
BbeI BbrPI BcgI BciVI BclI
BfaI BfrI BglII BlnI BsaAI
BsiWI BsmBI BsmI BspHI BspLU11I
BspMI BsrDI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
CpoI DraI DrdI Eco47III Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HpaI
HphI KasI KpnI KspI MaeI
MfeI MluI MseI MunI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
RcaI RsrII SacII SalI SapI
ScaI SexAI SfoI SfuI SnaBI
SpeI SphI SspI SwaI TfiI
Tru9I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AccIII 338 TCCGGA T^CCGGA
AflIII 701 ACGTGT A^C[GA][CT]GT
AhdI 670 GACCGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 232 GTGCAC G^TGCAC
AlwI 371 GGATC GGATC(4/5)
ApaLI 232 GTGCAC G^TGCAC
AspEI 670 GACCGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 775 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 797 CCCGGG C^[CT]CG[GA]G
BalI 1220 TGGCCA TGG^CCA
BbsI 361 GAAGAC GAAGAC(2/6)
BlpI 39 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 39 GCTGAGC GC^T[ACGT]AGC
BpuAI 361 GAAGAC GAAGAC(2/6)
BsaBI 504 GATGGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 268 GAGACC GGTCTC(1/5)
BsaWI 338 TCCGGA [AT]^CCGG[AT]
BseAI 338 TCCGGA T^CCGGA
BsoBI 797 CCCGGG C^[CT]CG[GA]G
Bsp1407I 590 TGTACA T^GTACA
BspDI 253 ATCGAT AT^CGAT
BspEI 338 TCCGGA T^CCGGA
BsrGI 590 TGTACA T^GTACA
BstXI 272 CCATCGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 371 GGATCT [GA]^GATC[CT]
CelII 39 GCTGAGC GC^T[ACGT]AGC
ClaI 253 ATCGAT AT^CGAT
DraIII 697 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 670 GACCGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HaeII 1066 AGCGCC [GA]GCGC^[CT]
HindIII 1018 AAGCTT A^AGCTT
MaeIII 825 GTCAC ^GT[ACGT]AC
MamI 504 GATGGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 371 GGATCT [GA]^GATC[CT]
MluNI 1220 TGGCCA TGG^CCA
MroI 338 TCCGGA T^CCGGA
MscI 1220 TGGCCA TGG^CCA
MslI 972 CACAGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 118 CAGCTG C[AC]G^C[GT]G
NcoI 977 CCATGG C^CATGG
NspI 1060 ACATGC [GA]CATG^[CT]
PflFI 775 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 216 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 958 CTGCAG CTGCA^G
PvuI 137 CGATCG CGAT^CG
PvuII 118 CAGCTG CAG^CTG
SbfI 957 CCTGCAGG CCTGCA^GG
SfcI 958 CTGCAG C^T[GA][CT]AG
SgrAI 1176 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 797 CCCGGG CCC^GGG
SmlI 643 CTCAAG C^T[CT][GA]AG
Sse8387I 957 CCTGCAGG CCTGCA^GG
SspBI 590 TGTACA T^GTACA
Tsp45I 825 GTCAC ^GT[GC]AC
Tth111I 775 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 216 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 371 GGATCT [GA]^GATC[CT]
XmaCI 797 CCCGGG C^CCGGG
XmaI 797 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 39 enzymes) #
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AatII 160 GACGTC GACGT^C
184 GACGTC
AccII 1107 CGCG CG^CG
1131 CGCG
AcsI 66 GAATTT [GA]^AATT[CT]
564 GAATTT
AcyI 160 GACGTC G[GA]^CG[CT]C
184 GACGTC
AfaI 249 GTAC GT^AC
591 GTAC
ApaI 150 GGGCCC GGGCC^C
800 GGGCCC
ApoI 66 GAATTT [GA]^AATT[CT]
564 GAATTT
BanI 487 GGCACC G^G[CT][GA]CC
1138 GGCACC
BbvCI 1005 GCTGAGG CCTCAGC(-5/-2)
1120 GCTGAGG
BpmI 223 CTGGAG CTGGAG(16/14)
433 CTGGAG
BsaHI 160 GACGTC G[GA]^CG[CT]C
184 GACGTC
BseRI 1233 CTCCTC GAGGAG(10/8)
142 GAGGAG
BsgI 90 GTGCAG GTGCAG(16/14)
115 GTGCAG
BsmAI 268 GAGAC GTCTC(1/5)
1161 GAGAC
BsmFI 992 GGGAC GGGAC(10/14)
219 GTCCC
BssSI 399 CACGAG CACGAG(-5/-1)
499 CACGAG
BstUI 1107 CGCG CG^CG
1131 CGCG
CfoI 1067 GCGC GCG^C
1108 GCGC
DsaI 623 CCGTGG C^C[GA][CT]GG
977 CCATGG
EagI 133 CGGCCG C^GGCCG
766 CGGCCG
EarI 829 CTCTTC CTCTTC(1/4)
21 GAAGAG
EclXI 133 CGGCCG C^GGCCG
766 CGGCCG
Eco52I 133 CGGCCG C^GGCCG
766 CGGCCG
EcoNI 588 CCTGTACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
804 CCTGCGGCAGG
EcoT14I 977 CCATGG C^C[AT][AT]GG
1226 CCAAGG
HgaI 1105 GACGC GACGC(5/10)
1129 GACGC
HhaI 1067 GCGC GCG^C
1108 GCGC
Hin1I 160 GACGTC G[GA]^CG[CT]C
184 GACGTC
HinP1I 1067 GCGC G^CGC
1108 GCGC
Ksp632I 829 CTCTTC CTCTTC(1/4)
21 GAAGAG
MvnI 1107 CGCG CG^CG
1131 CGCG
PshAI 180 GACTGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
270 GACCATCGTC
RsaI 249 GTAC GT^AC
591 GTAC
SfaNI 256 GATGC GCATC(5/9)
836 GCATC
SfiI 1212 GGCCTCCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1221 GGCCACCAAGGCC
StyI 977 CCATGG C^C[AT][AT]GG
1226 CCAAGG
Tsp509I 67 AATT ^AATT
565 AATT
TspRI 815 CACTG CA[GC]TG[ACGT][ACGT]^
951 CACTG
XcmI 422 CCATGCGGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
986 CCACAGGGGACGTGG
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# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AluI AlwNI AspHI AvaII
BanII BbvI BcnI BglI BmyI
BsaJI BsiEI BsiHKAI BsiYI BslI
Bsp1286I BsrBI BsrFI BsrI BssKI
BstF5I BstNI Cac8I Cfr10I Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII ItaI MaeII
MboI MboII MnlI MspI MvaI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV PleI SacI Sau3AI
Sau96I ScrFI StuI TaqI TseI
TthHB8I