Restriction map for
pFN21AE3655
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# NO CUT ENZYMES ( 75 enzymes) #
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AatII AccII AclI AcsI AflII
AhdI ApoI AscI AseI AsnI
AspEI AspI AviII AvrII BbeI
BciVI BclI BfrI BglII BlnI
BsaBI BsiWI Bsp1407I BspDI BspHI
BsrGI BssHII Bst1107I BstUI BstZ17I
ClaI CpoI DraI Eam1105I EcoRI
EcoRV FbaI FspI HindIII HpaI
KasI KspI MamI MfeI MluI
MseI MunI MvnI NarI NdeI
NotI NruI PacI PaeR7I PflFI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI ScaI SexAI
SfoI SgrAI SpeI SphI SspBI
SspI SwaI Tru9I Tth111I XhoI
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# ONE CUT ENZYMES ( 56 enzymes) #
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Acc65I 2754 GGTACC G^GTACC
AccI 1466 GTAGAC GT^[AC][GT]AC
AccIII 1185 TCCGGA T^CCGGA
AcyI 1292 GACGCC G[GA]^CG[CT]C
AflIII 1728 ACATGT A^C[GA][CT]GT
AgeI 2505 ACCGGT A^CCGGT
Aor51HI 1418 AGCGCT AGC^GCT
Asp700I 2917 GAACTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2754 GGTACC G^GTACC
BbrPI 685 CACGTG CAC^GTG
BbsI 1181 GTCTTC GAAGAC(2/6)
BpuAI 1181 GTCTTC GAAGAC(2/6)
BsaHI 1292 GACGCC G[GA]^CG[CT]C
BseAI 1185 TCCGGA T^CCGGA
BsiEI 2864 CGGCCG CG[GA][CT]^CG
BsmBI 1290 GAGACG CGTCTC(1/5)
BsmI 569 GCATTC GAATGC(1/-1)
BspEI 1185 TCCGGA T^CCGGA
BspLU11I 1728 ACATGT A^CATGT
BsrBI 1531 CCGCTC CCGCTC(-3/-3)
BsrDI 2556 CATTGC GCAATG(2/0)
BssSI 460 CTCGTG CACGAG(-5/-1)
BstBI 18 TTCGAA TT^CGAA
BstEII 2873 GGTCACC G^GT[ACGT]ACC
BstPI 2873 GGTCACC G^GT[ACGT]ACC
DraIII 408 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2778 GACCCTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2864 CGGCCG C^GGCCG
EclXI 2864 CGGCCG C^GGCCG
Eco47III 1418 AGCGCT AGC^GCT
Eco52I 2864 CGGCCG C^GGCCG
EcoNI 867 CCTGCATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2873 GGTCACC G^GT[ACGT]ACC
EcoT22I 2212 ATGCAT ATGCA^T
FseI 913 GGCCGGCC GGCCGG^CC
Hin1I 1292 GACGCC G[GA]^CG[CT]C
KpnI 2754 GGTACC GGTAC^C
MroI 1185 TCCGGA T^CCGGA
NaeI 914 GCCGGC GCC^GGC
NcoI 909 CCATGG C^CATGG
NgoMIV 914 GCCGGC G^CCGGC
NheI 2473 GCTAGC G^CTAGC
NsiI 2212 ATGCAT ATGCA^T
NspI 1728 ACATGT [GA]CATG^[CT]
NspV 18 TTCGAA TT^CGAA
PinAI 2505 ACCGGT A^CCGGT
PleI 2785 GAGTC GAGTC(4/5)
PmaCI 685 CACGTG CAC^GTG
PmlI 685 CACGTG CAC^GTG
PshAI 788 GACATAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 117 CCTGCAGG CCTGCA^GG
SfuI 18 TTCGAA TT^CGAA
SnaBI 2179 TACGTA TAC^GTA
Sse8387I 117 CCTGCAGG CCTGCA^GG
XbaI 2316 TCTAGA T^CTAGA
XmnI 2917 GAACTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
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Alw44I 316 GTGCAC G^TGCAC
884 GTGCAC
AocI 1213 CCTGAGG CC^T[ACGT]AGG
2904 CCTCAGG
ApaI 1841 GGGCCC GGGCC^C
2397 GGGCCC
ApaLI 316 GTGCAC G^TGCAC
884 GTGCAC
BbvCI 1757 CCTCAGC CCTCAGC(-5/-2)
2064 GCTGAGG
BsaAI 685 CACGTG [CT]AC^GT[GA]
2179 TACGTA
BsaI 771 GAGACC GGTCTC(1/5)
1882 GAGACC
BsaWI 1185 TCCGGA [AT]^CCGG[AT]
2505 ACCGGT
BsrFI 914 GCCGGC [GA]^CCGG[CT]
2505 ACCGGT
Bsu36I 1213 CCTGAGG CC^T[ACGT]AGG
2904 CCTCAGG
Cfr10I 914 GCCGGC [GA]^CCGG[CT]
2505 ACCGGT
DsaI 909 CCATGG C^C[GA][CT]GG
2623 CCACGG
Eco81I 1213 CCTGAGG CC^T[ACGT]AGG
2904 CCTCAGG
HincII 1399 GTTGAC GT[CT]^[GA]AC
2509 GTCAAC
HindII 1399 GTTGAC GT[CT]^[GA]AC
2509 GTCAAC
SapI 1000 GAAGAGC GCTCTTC(1/4)
1012 GAAGAGC
TaqI 19 TCGA T^CGA
1175 TCGA
TfiI 87 GATTC G^A[AT]TC
2499 GATTC
TthHB8I 19 TCGA T^CGA
1175 TCGA
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# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaI AvaII BalI BamHI
BanI BanII BbvI BcgI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstAPI
BstF5I BstNI BstXI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PflMI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SfiI
SmaI SmlI StuI StyI TseI
Tsp45I Tsp509I TspRI Van91I XcmI
XhoII XmaCI XmaI