Restriction map for
pFN21AE3616
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
AspEI AspI AvaI AviII AvrII
BamHI BbeI BbvCI BcgI BciVI
BclI BfrI BglI BlnI BlpI
Bpu1102I BsaBI BsaHI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstUI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HpaI KasI KspI
MamI MluI MroI MslI MspA1I
MvnI NaeI NarI NdeI NgoMIV
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SspBI StuI
SwaI Tth111I Van91I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 1146 GGTACC G^GTACC
Asp718I 1146 GGTACC G^GTACC
AspHI 787 GTGCTC G[AT]GC[AT]^C
AvaII 1239 GGACC G^G[AT]CC
BalI 486 TGGCCA TGG^CCA
BanII 854 GGGCTC G[GA]GC[CT]^C
BbrPI 868 CACGTG CAC^GTG
BbvI 120 GCAGC GCAGC(8/12)
BcnI 15 CCCGG CC^[GC]GG
BsaAI 868 CACGTG [CT]AC^GT[GA]
BsiHKAI 787 GTGCTC G[AT]GC[AT]^C
BsmFI 831 GTCCC GGGAC(10/14)
BsmI 1004 GCATTC GAATGC(1/-1)
BsrDI 230 CATTGC GCAATG(2/0)
BstAPI 213 GCACAGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 388 GGTCACC G^GT[ACGT]ACC
BstPI 388 GGTCACC G^GT[ACGT]ACC
DraII 1238 AGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 529 CCATGG C^C[GA][CT]GG
EaeI 486 TGGCCA [CT]^GGCC[GA]
EcoO109I 1238 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 388 GGTCACC G^GT[ACGT]ACC
FauI 589 CCCGC CCCGC(4/6)
HapII 16 CCGG C^CGG
HincII 240 GTCAAC GT[CT]^[GA]AC
HindII 240 GTCAAC GT[CT]^[GA]AC
HindIII 689 AAGCTT A^AGCTT
HpaII 16 CCGG C^CGG
KpnI 1146 GGTACC GGTAC^C
MaeII 869 ACGT A^CGT
MfeI 520 CAATTG C^AATTG
MluNI 486 TGGCCA TGG^CCA
MscI 486 TGGCCA TGG^CCA
MspI 16 CCGG C^CGG
MunI 520 CAATTG C^AATTG
NciI 15 CCCGG CC^[GC]GG
NcoI 529 CCATGG C^CATGG
NheI 802 GCTAGC G^CTAGC
PmaCI 868 CACGTG CAC^GTG
PmlI 868 CACGTG CAC^GTG
PpuMI 1238 AGGACCC [GA]G^G[AT]CC[CT]
SapI 437 GAAGAGC GCTCTTC(1/4)
SmlI 1217 CTCAAG C^T[CT][GA]AG
SpeI 508 ACTAGT A^CTAGT
SspI 1123 AATATT AAT^ATT
TaqI 1335 TCGA T^CGA
TseI 120 GCAGC G^C[AT]GC
TthHB8I 1335 TCGA T^CGA
XcmI 290 CCACAGCAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Asp700I 58 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1033 GAAATAATTC
BanI 18 GGTGCC G^G[CT][GA]CC
1146 GGTACC
BglII 34 AGATCT A^GATCT
1137 AGATCT
BpmI 315 CTCCAG CTGGAG(16/14)
1253 CTGGAG
BsaI 533 GGTCTC GGTCTC(1/5)
756 GGTCTC
BsaJI 134 CCAAGG C^C[ACGT][ACGT]GG
529 CCATGG
BsiYI 9 CCTCAACCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
846 CCCTCATGGGG
BslI 9 CCTCAACCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
846 CCCTCATGGGG
BspMI 361 GCAGGT ACCTGC(4/8)
442 GCAGGT
BstYI 34 AGATCT [GA]^GATC[CT]
1137 AGATCT
Cfr13I 607 GGGCC G^G[ACGT]CC
1239 GGACC
DpnII 35 GATC ^GATC
1138 GATC
DraI 105 TTTAAA TTT^AAA
1245 TTTAAA
EcoT14I 134 CCAAGG C^C[AT][AT]GG
529 CCATGG
Fnu4HI 120 GCAGC GC^[ACGT]GC
210 GCCGC
ItaI 120 GCAGC GC^[ACGT]GC
210 GCCGC
MboI 35 GATC ^GATC
1138 GATC
MflI 34 AGATCT [GA]^GATC[CT]
1137 AGATCT
NdeII 35 GATC ^GATC
1138 GATC
PleI 781 GAGTC GAGTC(4/5)
829 GAGTC
Sau3AI 35 GATC ^GATC
1138 GATC
Sau96I 607 GGGCC G^G[ACGT]CC
1239 GGACC
StyI 134 CCAAGG C^C[AT][AT]GG
529 CCATGG
XhoII 34 AGATCT [GA]^GATC[CT]
1137 AGATCT
XmnI 58 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1033 GAAATAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BbsI BfaI BmyI BpuAI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I DdeI EarI EcoRII FokI
HaeIII HinfI HphI Ksp632I MaeI
MaeIII MboII MnlI MseI MvaI
MwoI NlaIII NlaIV RsaI ScrFI
SfaNI SfcI TfiI Tru9I Tsp45I
Tsp509I TspRI