Restriction map for
pFN21AE3605
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII AccI AclI AcyI AflII
AgeI AhdI Aor51HI ApaI AscI
AseI AsnI Asp700I AspEI AspI
AviII BamHI BbeI BbrPI BbsI
BbvCI BcgI BclI BfrI BpuAI
BsaAI BsaBI BsaHI BsaI BsiEI
BsmAI BsmBI BsmI BspDI BspHI
BsrBI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoO65I EcoRV EcoT22I FauI FbaI
FseI FspI HaeII Hin1I HpaI
KasI KspI MamI MfeI MslI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SspI StuI
SwaI Tth111I XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 1233 GGTACC G^GTACC
AccIII 264 TCCGGA T^CCGGA
AciI 1240 GCGG CCGC(-3/-1)
Alw44I 1270 GTGCAC G^TGCAC
AlwNI 1072 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 889 CCTGAGG CC^T[ACGT]AGG
ApaLI 1270 GTGCAC G^TGCAC
Asp718I 1233 GGTACC G^GTACC
AvaI 1138 CCCGAG C^[CT]CG[GA]G
AvrII 49 CCTAGG C^CTAGG
BalI 242 TGGCCA TGG^CCA
BanI 1233 GGTACC G^G[CT][GA]CC
BciVI 261 GTATCC GTATCC(6/5)
BcnI 1113 CCGGG CC^[GC]GG
BglI 312 GCCTTTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 896 AGATCT A^GATCT
BlnI 49 CCTAGG C^CTAGG
BlpI 1304 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 1304 GCTTAGC GC^T[ACGT]AGC
BseAI 264 TCCGGA T^CCGGA
BsiWI 227 CGTACG C^GTACG
BsoBI 1138 CCCGAG C^[CT]CG[GA]G
BspEI 264 TCCGGA T^CCGGA
BspLU11I 1118 ACATGT A^CATGT
BsrDI 780 GCAATG GCAATG(2/0)
BstXI 440 CCAGTGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 889 CCTGAGG CC^T[ACGT]AGG
CelII 1304 GCTTAGC GC^T[ACGT]AGC
CfoI 1018 GCGC GCG^C
EaeI 242 TGGCCA [CT]^GGCC[GA]
EarI 125 GAAGAG CTCTTC(1/4)
Eco81I 889 CCTGAGG CC^T[ACGT]AGG
EcoRI 350 GAATTC G^AATTC
HhaI 1018 GCGC GCG^C
HinP1I 1018 GCGC G^CGC
HincII 130 GTCAAC GT[CT]^[GA]AC
HindII 130 GTCAAC GT[CT]^[GA]AC
KpnI 1233 GGTACC GGTAC^C
Ksp632I 125 GAAGAG CTCTTC(1/4)
MaeII 226 ACGT A^CGT
MluI 22 ACGCGT A^CGCGT
MluNI 242 TGGCCA TGG^CCA
MroI 264 TCCGGA T^CCGGA
MscI 242 TGGCCA TGG^CCA
NciI 1113 CCGGG CC^[GC]GG
PshAI 1159 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 900 CTGCAG CTGCA^G
SfaNI 862 GATGC GCATC(5/9)
SpeI 277 ACTAGT A^CTAGT
TaqI 114 TCGA T^CGA
TfiI 492 GAATC G^A[AT]TC
TthHB8I 114 TCGA T^CGA
XbaI 834 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 23 CGCG CG^CG
1019 CGCG
AcsI 350 GAATTC [GA]^AATT[CT]
616 GAATTT
AflIII 22 ACGCGT A^C[GA][CT]GT
1118 ACATGT
AlwI 91 GGATC GGATC(4/5)
1146 GGATC
ApoI 350 GAATTC [GA]^AATT[CT]
616 GAATTT
BanII 12 GAGCCC G[GA]GC[CT]^C
1178 GGGCTC
BsaJI 49 CCTAGG C^C[ACGT][ACGT]GG
500 CCAAGG
BsaWI 264 TCCGGA [AT]^CCGG[AT]
1311 ACCGGA
BsgI 811 GTGCAG GTGCAG(16/14)
1128 GTGCAG
BsmFI 785 GGGAC GGGAC(10/14)
1115 GGGAC
Bsp1407I 1004 TGTACA T^GTACA
1219 TGTACA
BspMI 1236 ACCTGC ACCTGC(4/8)
902 GCAGGT
BsrGI 1004 TGTACA T^GTACA
1219 TGTACA
BstUI 23 CGCG CG^CG
1019 CGCG
BstYI 91 GGATCT [GA]^GATC[CT]
896 AGATCT
EcoT14I 49 CCTAGG C^C[AT][AT]GG
500 CCAAGG
HgaI 471 GACGC GACGC(5/10)
24 GCGTC
HphI 468 GGTGA GGTGA(8/7)
729 GGTGA
MflI 91 GGATCT [GA]^GATC[CT]
896 AGATCT
MvnI 23 CGCG CG^CG
1019 CGCG
NlaIV 29 GGGTCC GG[ACGT]^[ACGT]CC
1233 GGTACC
NspI 999 GCATGT [GA]CATG^[CT]
1118 ACATGT
PflMI 418 CCAAGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1071 CCAGCTGCTGG
PleI 128 GAGTC GAGTC(4/5)
948 GAGTC
ScaI 274 AGTACT AGT^ACT
792 AGTACT
SspBI 1004 TGTACA T^GTACA
1219 TGTACA
StyI 49 CCTAGG C^C[AT][AT]GG
500 CCAAGG
Van91I 418 CCAAGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1071 CCAGCTGCTGG
XcmI 677 CCAACAGTTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1287 CCAGAGGAGTGATGG
XhoII 91 GGATCT [GA]^GATC[CT]
896 AGATCT
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# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AfaI AluI AspHI AvaII BbvI
BfaI BmyI BpmI BseRI BsiHKAI
BsiYI BslI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EcoRII Fnu4HI FokI HaeIII
HapII HindIII HinfI HpaII ItaI
MaeI MaeIII MboI MboII MnlI
MseI MspA1I MspI MvaI MwoI
NdeII NlaIII PvuII RsaI Sau3AI
Sau96I ScrFI SfcI SmlI Tru9I
TseI Tsp45I Tsp509I TspRI