Restriction map for
pFN21AE1897
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AhdI Alw44I AocI Aor51HI ApaLI
AscI AspEI AspHI AspI AvaI
AviII AvrII BamHI BbeI BbvCI
BcgI BcnI BglI BglII BlnI
BlpI BpmI Bpu1102I BsaBI BsaHI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrDI BsrGI BssHII
BssKI BssSI Bst1107I BstAPI BstBI
BstEII BstNI BstPI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRII
FseI FspI Hin1I KasI KspI
MamI MfeI MluI MroI MslI
MunI MvaI NaeI NarI NciI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PmeI PpuMI
PshAI Psp1406I PvuI RsrII SacI
SacII SalI SbfI ScaI ScrFI
SexAI SfiI SfoI SfuI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Tth111I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccII 1342 CGCG CG^CG
AflIII 1510 ACACGT A^C[GA][CT]GT
AgeI 448 ACCGGT A^CCGGT
ApaI 5 GGGCCC GGGCC^C
AseI 2059 ATTAAT AT^TAAT
AsnI 2059 ATTAAT AT^TAAT
AvaII 1628 GGTCC G^G[AT]CC
BbrPI 1181 CACGTG CAC^GTG
BciVI 783 GGATAC GTATCC(6/5)
BsaAI 1181 CACGTG [CT]AC^GT[GA]
BsaI 1032 GAGACC GGTCTC(1/5)
BsaWI 448 ACCGGT [AT]^CCGG[AT]
BseRI 868 GAGGAG GAGGAG(10/8)
BsmI 2222 GAATGC GAATGC(1/-1)
BspHI 1982 TCATGA T^CATGA
BspMI 428 ACCTGC ACCTGC(4/8)
BsrFI 448 ACCGGT [GA]^CCGG[CT]
BstUI 1342 CGCG CG^CG
Cac8I 1945 GCCAGC GC[ACGT]^[ACGT]GC
Cfr10I 448 ACCGGT [GA]^CCGG[CT]
DraI 522 TTTAAA TTT^AAA
DrdI 42 GACCTTCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 1012 GATATC GAT^ATC
EcoT22I 185 ATGCAT ATGCA^T
HaeII 1913 AGCGCC [GA]GCGC^[CT]
HapII 449 CCGG C^CGG
HgaI 32 GCGTC GACGC(5/10)
HpaI 244 GTTAAC GTT^AAC
HpaII 449 CCGG C^CGG
HphI 1374 GGTGA GGTGA(8/7)
MspI 449 CCGG C^CGG
MvnI 1342 CGCG CG^CG
NdeI 2201 CATATG CA^TATG
NsiI 185 ATGCAT ATGCA^T
PflMI 1575 CCAGTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 448 ACCGGT A^CCGGT
PmaCI 1181 CACGTG CAC^GTG
PmlI 1181 CACGTG CAC^GTG
PshBI 2059 ATTAAT AT^TAAT
PstI 929 CTGCAG CTGCA^G
PvuII 2390 CAGCTG CAG^CTG
RcaI 1982 TCATGA T^CATGA
SapI 2269 GAAGAGC GCTCTTC(1/4)
SgrAI 447 CACCGGTG C[GA]^CCGG[CT]G
SspI 809 AATATT AAT^ATT
TspRI 356 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 1575 CCAGTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 464 CCAGAGAAGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Acc65I 490 GGTACC G^GTACC
1465 GGTACC
AflII 1369 CTTAAG C^TTAAG
1879 CTTAAG
AlwNI 1706 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
1912 CAGCGCCTG
Asp700I 160 GAAGCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1755 GAAGAGGTTC
Asp718I 490 GGTACC G^GTACC
1465 GGTACC
BanI 490 GGTACC G^G[CT][GA]CC
1465 GGTACC
BclI 1412 TGATCA T^GATCA
2078 TGATCA
BfrI 1369 CTTAAG C^TTAAG
1879 CTTAAG
BsmFI 508 GGGAC GGGAC(10/14)
992 GGGAC
CfoI 1239 GCGC GCG^C
1914 GCGC
DsaI 875 CCATGG C^C[GA][CT]GG
1106 CCATGG
FauI 1340 CCCGC CCCGC(4/6)
2073 CCCGC
FbaI 1412 TGATCA T^GATCA
2078 TGATCA
HhaI 1239 GCGC GCG^C
1914 GCGC
HinP1I 1239 GCGC G^CGC
1914 GCGC
HincII 244 GTTAAC GT[CT]^[GA]AC
558 GTCAAC
HindII 244 GTTAAC GT[CT]^[GA]AC
558 GTCAAC
KpnI 490 GGTACC GGTAC^C
1465 GGTACC
MaeII 1182 ACGT A^CGT
1512 ACGT
MspA1I 2074 CCGCTG C[AC]G^C[GT]G
2390 CAGCTG
NcoI 875 CCATGG C^CATGG
1106 CCATGG
NspI 435 GCATGT [GA]CATG^[CT]
2087 ACATGC
SfcI 929 CTGCAG C^T[GA][CT]AG
2387 CTACAG
SmlI 1369 CTTAAG C^T[CT][GA]AG
1879 CTTAAG
Tsp45I 439 GTCAC ^GT[GC]AC
631 GTCAC
XmnI 160 GAAGCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1755 GAAGAGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BalI BanII BbsI BbvI
BfaI BmyI BpuAI BsaJI BsiYI
BslI BsmAI Bsp1286I BsrI BstF5I
BstYI Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoT14I Fnu4HI
FokI HaeIII HindIII HinfI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MwoI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I SfaNI StyI
TaqI TfiI Tru9I TseI Tsp509I
TthHB8I XhoII