Restriction map for
pFN21AE2079
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# NO CUT ENZYMES ( 113 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AgeI AhdI Alw44I ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BamHI BbeI
BbrPI BciVI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I CelII Cfr10I ClaI
CpoI DraI DraIII EagI Eam1105I
EclXI Eco52I EcoO65I EcoRV EcoT22I
FseI FspI Hin1I HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MunI NaeI NarI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI Van91I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccII 1528 CGCG CG^CG
AflII 1885 CTTAAG C^TTAAG
AflIII 1400 ACATGT A^C[GA][CT]GT
AocI 637 CCTGAGG CC^T[ACGT]AGG
Aor51HI 743 AGCGCT AGC^GCT
AspHI 544 GTGCTC G[AT]GC[AT]^C
AspI 511 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 444 TGGCCA TGG^CCA
BanI 97 GGCACC G^G[CT][GA]CC
BcgI 536 CGAATCTTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 956 TGATCA T^GATCA
BfaI 1276 CTAG C^TAG
BfrI 1885 CTTAAG C^TTAAG
BsiHKAI 544 GTGCTC G[AT]GC[AT]^C
BspLU11I 1400 ACATGT A^CATGT
BssSI 2174 CACGAG CACGAG(-5/-1)
BstUI 1528 CGCG CG^CG
Bsu36I 637 CCTGAGG CC^T[ACGT]AGG
DrdI 1495 GACCAATGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 444 TGGCCA [CT]^GGCC[GA]
Eco47III 743 AGCGCT AGC^GCT
Eco81I 637 CCTGAGG CC^T[ACGT]AGG
EcoNI 2210 CCTGGCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1594 GAATTC G^AATTC
FbaI 956 TGATCA T^GATCA
HgaI 384 GCGTC GACGC(5/10)
HincII 1165 GTTGAC GT[CT]^[GA]AC
HindII 1165 GTTGAC GT[CT]^[GA]AC
MaeI 1276 CTAG C^TAG
MluNI 444 TGGCCA TGG^CCA
MscI 444 TGGCCA TGG^CCA
MvnI 1528 CGCG CG^CG
NcoI 114 CCATGG C^CATGG
NdeI 1351 CATATG CA^TATG
PflFI 511 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1956 GACAGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1315 CTGCAG CTGCA^G
SexAI 1337 ACCAGGT A^CC[AT]GGT
SfaNI 178 GCATC GCATC(5/9)
SmaI 938 CCCGGG CCC^GGG
Tth111I 511 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1382 CCATCTGCGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 938 CCCGGG C^CCGGG
XmaI 938 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
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AcsI 1594 GAATTC [GA]^AATT[CT]
2241 GAATTT
AfaI 39 GTAC GT^AC
165 GTAC
AlwI 1182 GATCC GGATC(4/5)
848 GGATC
ApoI 1594 GAATTC [GA]^AATT[CT]
2241 GAATTT
AvaI 90 CTCGGG C^[CT]CG[GA]G
938 CCCGGG
BbsI 705 GAAGAC GAAGAC(2/6)
555 GTCTTC
BbvCI 108 CCTCAGC CCTCAGC(-5/-2)
2214 GCTGAGG
BpuAI 705 GAAGAC GAAGAC(2/6)
555 GTCTTC
BseRI 531 CTCCTC GAGGAG(10/8)
1066 CTCCTC
BsgI 1333 CTGCAC GTGCAG(16/14)
1749 CTGCAC
BsoBI 90 CTCGGG C^[CT]CG[GA]G
938 CCCGGG
BspHI 990 TCATGA T^CATGA
1043 TCATGA
BspMI 767 ACCTGC ACCTGC(4/8)
1471 ACCTGC
BsrBI 562 GAGCGG CCGCTC(-3/-3)
2056 GAGCGG
BsrDI 1519 CATTGC GCAATG(2/0)
332 GCAATG
BstYI 848 GGATCT [GA]^GATC[CT]
1181 AGATCC
EarI 2194 CTCTTC CTCTTC(1/4)
1200 GAAGAG
EcoT14I 114 CCATGG C^C[AT][AT]GG
2115 CCTTGG
HaeII 743 AGCGCT [GA]GCGC^[CT]
826 AGCGCC
Ksp632I 2194 CTCTTC CTCTTC(1/4)
1200 GAAGAG
MaeII 80 ACGT A^CGT
644 ACGT
MaeIII 1444 GTCAC ^GT[ACGT]AC
1975 GTGAC
MflI 848 GGATCT [GA]^GATC[CT]
1181 AGATCC
PvuII 422 CAGCTG CAG^CTG
1160 CAGCTG
RcaI 990 TCATGA T^CATGA
1043 TCATGA
RsaI 39 GTAC GT^AC
165 GTAC
SfcI 1315 CTGCAG C^T[GA][CT]AG
1797 CTGTAG
SphI 1302 GCATGC GCATG^C
2201 GCATGC
StyI 114 CCATGG C^C[AT][AT]GG
2115 CCTTGG
TaqI 344 TCGA T^CGA
535 TCGA
Tsp45I 1444 GTCAC ^GT[GC]AC
1975 GTGAC
Tsp509I 1595 AATT ^AATT
2242 AATT
TthHB8I 344 TCGA T^CGA
535 TCGA
XhoII 848 GGATCT [GA]^GATC[CT]
1181 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AluI AlwNI ApaI AvaII
BanII BbvI BcnI BglI BmyI
BpmI BsaI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstXI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI Sau3AI
Sau96I ScrFI SmlI TfiI Tru9I
TseI TspRI