Restriction map for
pFN21AE0399
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# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII AccIII AclI AflII AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp700I AspEI AviII AvrII
BbrPI BcgI BfrI BlnI BlpI
Bpu1102I BseAI BsiEI BsiWI BspDI
BspEI BspHI BsrBI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I CelII
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I FseI FspI HgaI HpaI
KspI MluI MroI NaeI NdeI
NgoMIV NotI NruI NspV PacI
PaeR7I PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI SexAI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I SspI SwaI XhoI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 1628 GTAGAC GT^[AC][GT]AC
AccII 2125 CGCG CG^CG
AgeI 660 ACCGGT A^CCGGT
AlwNI 2609 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1720 CCTCAGG CC^T[ACGT]AGG
AspI 141 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2889 TGGCCA TGG^CCA
BamHI 2253 GGATCC G^GATCC
BbsI 1114 GAAGAC GAAGAC(2/6)
BclI 1287 TGATCA T^GATCA
BglII 1095 AGATCT A^GATCT
BpuAI 1114 GAAGAC GAAGAC(2/6)
BsaAI 1488 CACGTA [CT]AC^GT[GA]
BsaBI 1267 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 660 ACCGGT [AT]^CCGG[AT]
BsmBI 139 GAGACG CGTCTC(1/5)
BsmI 1966 GAATGC GAATGC(1/-1)
Bsp1407I 1910 TGTACA T^GTACA
BspMI 3258 ACCTGC ACCTGC(4/8)
BsrFI 660 ACCGGT [GA]^CCGG[CT]
BsrGI 1910 TGTACA T^GTACA
BssSI 2234 CACGAG CACGAG(-5/-1)
BstUI 2125 CGCG CG^CG
Bsu36I 1720 CCTCAGG CC^T[ACGT]AGG
Cfr10I 660 ACCGGT [GA]^CCGG[CT]
Eco81I 1720 CCTCAGG CC^T[ACGT]AGG
EcoRI 2016 GAATTC G^AATTC
EcoRV 556 GATATC GAT^ATC
FbaI 1287 TGATCA T^GATCA
MaeII 1489 ACGT A^CGT
MamI 1267 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 486 CAATTG C^AATTG
MluNI 2889 TGGCCA TGG^CCA
MscI 2889 TGGCCA TGG^CCA
MunI 486 CAATTG C^AATTG
MvnI 2125 CGCG CG^CG
NheI 1045 GCTAGC G^CTAGC
PflFI 141 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 119 CCAAGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 660 ACCGGT A^CCGGT
SmlI 2011 CTTGAG C^T[CT][GA]AG
SphI 3155 GCATGC GCATG^C
SspBI 1910 TGTACA T^GTACA
StuI 78 AGGCCT AGG^CCT
Tth111I 141 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 119 CCAAGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 2184 GGTACC G^GTACC
3192 GGTACC
AcyI 43 GGCGCC G[GA]^CG[CT]C
346 GGCGCC
ApaI 108 GGGCCC GGGCC^C
2296 GGGCCC
Asp718I 2184 GGTACC G^GTACC
3192 GGTACC
AspHI 1406 GTGCTC G[AT]GC[AT]^C
2858 GAGCAC
BbeI 43 GGCGCC GGCGC^C
346 GGCGCC
BbvCI 2624 CCTCAGC CCTCAGC(-5/-2)
3302 CCTCAGC
BciVI 2478 GGATAC GTATCC(6/5)
3219 GTATCC
BglI 4 GCCGCAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2623 GCCTCAGCGGC
BsaHI 43 GGCGCC G[GA]^CG[CT]C
346 GGCGCC
BsgI 244 GTGCAG GTGCAG(16/14)
478 GTGCAG
BsiHKAI 1406 GTGCTC G[AT]GC[AT]^C
2858 GAGCAC
BspLU11I 1941 ACATGT A^CATGT
2530 ACATGT
BsrDI 2283 CATTGC GCAATG(2/0)
1639 GCAATG
EcoNI 1195 CCTGTTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1276 CCTGCCAAAGG
EcoT22I 1361 ATGCAT ATGCA^T
1968 ATGCAT
HaeII 43 GGCGCC [GA]GCGC^[CT]
346 GGCGCC
Hin1I 43 GGCGCC G[GA]^CG[CT]C
346 GGCGCC
HincII 1303 GTTGAC GT[CT]^[GA]AC
1615 GTTGAC
HindII 1303 GTTGAC GT[CT]^[GA]AC
1615 GTTGAC
HindIII 602 AAGCTT A^AGCTT
2314 AAGCTT
KasI 43 GGCGCC G^GCGCC
346 GGCGCC
KpnI 2184 GGTACC GGTAC^C
3192 GGTACC
NarI 43 GGCGCC GG^CGCC
346 GGCGCC
NcoI 784 CCATGG C^CATGG
1634 CCATGG
NsiI 1361 ATGCAT ATGCA^T
1968 ATGCAT
ScaI 1149 AGTACT AGT^ACT
2089 AGTACT
SfoI 43 GGCGCC GGC^GCC
346 GGCGCC
SmaI 3073 CCCGGG CCC^GGG
3188 CCCGGG
XbaI 1221 TCTAGA T^CTAGA
2781 TCTAGA
XmaCI 3073 CCCGGG C^CCGGG
3188 CCCGGG
XmaI 3073 CCCGGG C^CCGGG
3188 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AvaI AvaII BanI
BanII BbvI BcnI BfaI BmyI
BpmI BsaI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstAPI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII