Restriction map for
pFN21AE1697
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI AlwI
AocI Aor51HI ApaI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AvrII BamHI BbvCI BcgI BciVI
BfaI BfrI BglII BlnI BsaBI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstYI
BstZ17I Bsu36I ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FseI HincII
HindII HindIII HpaI KpnI MaeI
MamI MfeI MflI MluI MroI
MseI MunI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
StyI SwaI TfiI Tru9I Tth111I
Van91I XbaI XcmI XhoI XhoII
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# ONE CUT ENZYMES ( 64 enzymes) #
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AcyI 657 GGCGCC G[GA]^CG[CT]C
AflIII 332 ACATGT A^C[GA][CT]GT
Alw44I 595 GTGCAC G^TGCAC
AlwNI 244 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 595 GTGCAC G^TGCAC
Asp700I 141 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 595 GTGCAC G[AT]GC[AT]^C
AvaI 653 CCCGGG C^[CT]CG[GA]G
AviII 368 TGCGCA TGC^GCA
BalI 530 TGGCCA TGG^CCA
BbeI 657 GGCGCC GGCGC^C
BbrPI 526 CACGTG CAC^GTG
BclI 752 TGATCA T^GATCA
BlpI 225 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 225 GCTGAGC GC^T[ACGT]AGC
BsaHI 657 GGCGCC G[GA]^CG[CT]C
BsaI 103 GAGACC GGTCTC(1/5)
BsgI 40 CTGCAC GTGCAG(16/14)
BsiHKAI 595 GTGCAC G[AT]GC[AT]^C
BsmFI 188 GGGAC GGGAC(10/14)
BsoBI 653 CCCGGG C^[CT]CG[GA]G
BspLU11I 332 ACATGT A^CATGT
BstAPI 216 GCACAATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 428 CCAGCATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 225 GCTGAGC GC^T[ACGT]AGC
DpnII 753 GATC ^GATC
DraIII 217 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 325 CTCTTC CTCTTC(1/4)
FbaI 752 TGATCA T^GATCA
FspI 368 TGCGCA TGC^GCA
HaeII 657 GGCGCC [GA]GCGC^[CT]
HgaI 377 GCGTC GACGC(5/10)
Hin1I 657 GGCGCC G[GA]^CG[CT]C
HinfI 424 GAGTC G^A[ACGT]TC
HphI 473 TCACC GGTGA(8/7)
KasI 657 GGCGCC G^GCGCC
Ksp632I 325 CTCTTC CTCTTC(1/4)
KspI 558 CCGCGG CCGC^GG
MaeIII 121 GTGAC ^GT[ACGT]AC
MboI 753 GATC ^GATC
MluNI 530 TGGCCA TGG^CCA
MscI 530 TGGCCA TGG^CCA
NaeI 174 GCCGGC GCC^GGC
NarI 657 GGCGCC GG^CGCC
NdeII 753 GATC ^GATC
NgoMIV 174 GCCGGC G^CCGGC
PleI 424 GAGTC GAGTC(4/5)
PmaCI 526 CACGTG CAC^GTG
PmlI 526 CACGTG CAC^GTG
PpuMI 188 GGGACCT [GA]G^G[AT]CC[CT]
PstI 763 CTGCAG CTGCA^G
SacII 558 CCGCGG CCGC^GG
Sau3AI 753 GATC ^GATC
SfcI 763 CTGCAG C^T[GA][CT]AG
SfoI 657 GGCGCC GGC^GCC
SmaI 653 CCCGGG CCC^GGG
SmlI 211 CTCAAG C^T[CT][GA]AG
StuI 575 AGGCCT AGG^CCT
TaqI 449 TCGA T^CGA
Tsp45I 121 GTGAC ^GT[GC]AC
TthHB8I 449 TCGA T^CGA
XmaCI 653 CCCGGG C^CCGGG
XmaI 653 CCCGGG C^CCGGG
XmnI 141 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
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AfaI 246 GTAC GT^AC
516 GTAC
BanI 554 GGCACC G^G[CT][GA]CC
657 GGCGCC
BbsI 145 GTCTTC GAAGAC(2/6)
379 GTCTTC
BcnI 653 CCCGG CC^[GC]GG
654 CCGGG
BglI 349 GCCCAGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
581 GCCAGGAGGGC
BpmI 436 CTGGAG CTGGAG(16/14)
622 CTGGAG
BpuAI 145 GTCTTC GAAGAC(2/6)
379 GTCTTC
BsaAI 313 TACGTG [CT]AC^GT[GA]
526 CACGTG
BsmAI 21 GAGAC GTCTC(1/5)
103 GAGAC
BspMI 761 ACCTGC ACCTGC(4/8)
269 GCAGGT
BsrFI 174 GCCGGC [GA]^CCGG[CT]
548 ACCGGC
BsrI 599 ACTGG ACTGG(1/-1)
513 CCAGT
BstF5I 321 CATCC GGATG(2/0)
522 CATCC
Cfr10I 174 GCCGGC [GA]^CCGG[CT]
548 ACCGGC
DraII 188 GGGACCT [GA]G^G[ACGT]CC[CT]
452 AGGGCCT
EaeI 510 CGGCCA [CT]^GGCC[GA]
530 TGGCCA
EcoO109I 188 GGGACCT [GA]G^G[ACGT]CC[CT]
452 AGGGCCT
FauI 354 GCGGG CCCGC(4/6)
358 GCGGG
FokI 321 CATCC GGATG(9/13)
522 CATCC
MaeII 314 ACGT A^CGT
527 ACGT
MslI 68 CACTAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
522 CATCCACGTG
NciI 653 CCCGG CC^[GC]GG
654 CCGGG
PvuII 183 CAGCTG CAG^CTG
388 CAGCTG
RsaI 246 GTAC GT^AC
516 GTAC
SfaNI 521 GCATC GCATC(5/9)
590 GCATC
Tsp509I 80 AATT ^AATT
254 AATT
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# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AccII AciI AluI AvaII BanII
BbvI BmyI BsaJI BseRI BsiYI
BslI Bsp1286I BssKI BstNI BstUI
Cac8I CfoI Cfr13I DdeI DsaI
EcoRII Fnu4HI HaeIII HapII HhaI
HinP1I HpaII ItaI MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NlaIII NlaIV NspI Sau96I ScrFI
SphI TseI TspRI