Restriction map for
pFN21AB6156
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# NO CUT ENZYMES ( 128 enzymes) #
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AatII AccII AclI AcyI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I AspEI AviII AvrII
BalI BbeI BbrPI BcgI BciVI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsgI
BsiWI BsmBI BsmFI Bsp1407I BspDI
BspHI BspLU11I BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI Eam1105I Eco47III Eco81I
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HaeII HgaI Hin1I HindIII
KasI KspI MamI MfeI MluI
MluNI MscI MspA1I MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacII SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Van91I XbaI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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Acc65I 29 GGTACC G^GTACC
AccI 5 GTCGAC GT^[AC][GT]AC
Asp718I 29 GGTACC G^GTACC
AspI 1237 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 533 CTCGAG C^[CT]CG[GA]G
BamHI 1828 GGATCC G^GATCC
BanI 29 GGTACC G^G[CT][GA]CC
BbsI 1366 GAAGAC GAAGAC(2/6)
BclI 741 TGATCA T^GATCA
BpuAI 1366 GAAGAC GAAGAC(2/6)
BsmI 1302 GAATGC GAATGC(1/-1)
BsoBI 533 CTCGAG C^[CT]CG[GA]G
BspMI 648 GCAGGT ACCTGC(4/8)
BsrDI 1050 CATTGC GCAATG(2/0)
BsrI 1068 CCAGT ACTGG(1/-1)
BstXI 230 CCATAAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 84 GCGC GCG^C
DraII 1447 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoO109I 1447 AGGACCC [GA]G^G[ACGT]CC[CT]
FbaI 741 TGATCA T^GATCA
HhaI 84 GCGC GCG^C
HinP1I 84 GCGC G^CGC
HpaI 202 GTTAAC GTT^AAC
KpnI 29 GGTACC GGTAC^C
MseI 203 TTAA T^TAA
MslI 231 CATAAGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 1701 CCATGG C^CATGG
NspI 773 ACATGC [GA]CATG^[CT]
PaeR7I 533 CTCGAG C^TCGAG
PflFI 1237 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1447 AGGACCC [GA]G^G[AT]CC[CT]
SacI 1800 GAGCTC GAGCT^C
SalI 5 GTCGAC G^TCGAC
SexAI 614 ACCAGGT A^CC[AT]GGT
Tru9I 203 TTAA T^TAA
Tth111I 1237 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 533 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AlwNI 616 CAGGTACTG CAG[ACGT][ACGT][ACGT]^CTG
1396 CAGGAGCTG
AspHI 1515 GAGCAC G[AT]GC[AT]^C
1800 GAGCTC
BbvCI 1054 GCTGAGG CCTCAGC(-5/-2)
1099 GCTGAGG
BcnI 1782 CCGGG CC^[GC]GG
1824 CCGGG
BfaI 242 CTAG C^TAG
665 CTAG
BglII 1225 AGATCT A^GATCT
1253 AGATCT
BsiEI 1641 CGGCCG CG[GA][CT]^CG
1779 CGGCCG
BsiHKAI 1515 GAGCAC G[AT]GC[AT]^C
1800 GAGCTC
DsaI 1644 CCGTGG C^C[GA][CT]GG
1701 CCATGG
EagI 1641 CGGCCG C^GGCCG
1779 CGGCCG
EclXI 1641 CGGCCG C^GGCCG
1779 CGGCCG
Eco52I 1641 CGGCCG C^GGCCG
1779 CGGCCG
EcoNI 1350 CCTGCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1452 CCTCCTGGAGG
FauI 1376 CCCGC CCCGC(4/6)
1796 GCGGG
HincII 5 GTCGAC GT[CT]^[GA]AC
202 GTTAAC
HindII 5 GTCGAC GT[CT]^[GA]AC
202 GTTAAC
HphI 525 GGTGA GGTGA(8/7)
591 GGTGA
MaeI 242 CTAG C^TAG
665 CTAG
MaeII 255 ACGT A^CGT
1499 ACGT
NciI 1782 CCGGG CC^[GC]GG
1824 CCGGG
SapI 1089 GAAGAGC GCTCTTC(1/4)
1132 GAAGAGC
SmlI 533 CTCGAG C^T[CT][GA]AG
1309 CTCAAG
TfiI 757 GATTC G^A[AT]TC
1203 GAATC
XcmI 1361 CCAAGGAAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1412 CCAAGCTGGCCCTGG
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# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AccIII AciI AcsI AfaI AluI
AlwI ApoI AvaII BanII BbvI
BmyI BpmI BsaJI BsaWI BseAI
BseRI BsiYI BslI BsmAI Bsp1286I
BspEI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII EaeI
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HinfI HpaII ItaI
Ksp632I MaeIII MboI MboII MflI
MnlI MroI MspI MvaI MwoI
NdeII NlaIII NlaIV PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII