Restriction map for
pFN21AE1299
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# NO CUT ENZYMES ( 160 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
Aor51HI AscI AseI AsnI Asp700I
Asp718I AspI AvaI AviII AvrII
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BcnI BfaI BfrI
BglII BlnI BsaBI BsaHI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I CfoI Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EagI
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HindIII HpaI HpaII KasI
KpnI KspI MaeI MamI MfeI
MflI MluI MroI MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PstI PvuI PvuII RsrII SacII
SalI SapI SbfI ScaI SexAI
SfaNI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TaqI Tth111I TthHB8I Van91I XbaI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AfaI 114 GTAC GT^AC
AhdI 369 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 251 GTGCAC G^TGCAC
AlwI 3 GATCC GGATC(4/5)
AlwNI 647 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 337 CCTGAGG CC^T[ACGT]AGG
ApaLI 251 GTGCAC G^TGCAC
AspEI 369 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 135 TGGCCA TGG^CCA
BclI 501 TGATCA T^GATCA
BglI 683 GCCTCGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 441 GCTGAGC GC^T[ACGT]AGC
BpmI 184 CTGGAG CTGGAG(16/14)
Bpu1102I 441 GCTGAGC GC^T[ACGT]AGC
BsaAI 175 TACGTG [CT]AC^GT[GA]
BsaI 478 GAGACC GGTCTC(1/5)
BsmFI 128 GTCCC GGGAC(10/14)
BspHI 293 TCATGA T^CATGA
BsrDI 446 GCAATG GCAATG(2/0)
BssSI 685 CTCGTG CACGAG(-5/-1)
BstF5I 717 CATCC GGATG(2/0)
Bsu36I 337 CCTGAGG CC^T[ACGT]AGG
CelII 441 GCTGAGC GC^T[ACGT]AGC
EaeI 135 TGGCCA [CT]^GGCC[GA]
Eam1105I 369 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 596 CTCTTC CTCTTC(1/4)
Eco81I 337 CCTGAGG CC^T[ACGT]AGG
FauI 243 CCCGC CCCGC(4/6)
FbaI 501 TGATCA T^GATCA
FokI 717 CATCC GGATG(9/13)
HincII 427 GTCAAC GT[CT]^[GA]AC
HindII 427 GTCAAC GT[CT]^[GA]AC
HinfI 621 GATTC G^A[ACGT]TC
Ksp632I 596 CTCTTC CTCTTC(1/4)
MaeII 176 ACGT A^CGT
MluNI 135 TGGCCA TGG^CCA
MscI 135 TGGCCA TGG^CCA
MseI 299 TTAA T^TAA
MspA1I 317 CCGCTG C[AC]G^C[GT]G
MwoI 683 GCCTCGTGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PshAI 651 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 293 TCATGA T^CATGA
RsaI 114 GTAC GT^AC
SacI 640 GAGCTC GAGCT^C
StuI 723 AGGCCT AGG^CCT
TfiI 621 GATTC G^A[AT]TC
Tru9I 299 TTAA T^TAA
XcmI 147 CCATGAATTTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 15 enzymes) #
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AcsI 151 GAATTT [GA]^AATT[CT]
226 AAATTT
ApaI 29 GGGCCC GGGCC^C
757 GGGCCC
ApoI 151 GAATTT [GA]^AATT[CT]
226 AAATTT
BbsI 70 GAAGAC GAAGAC(2/6)
709 GTCTTC
BbvI 21 GCTGC GCAGC(8/12)
319 GCTGC
BpuAI 70 GAAGAC GAAGAC(2/6)
709 GTCTTC
BsiYI 58 CCCTGTGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
203 CCTGGCGGTGG
BslI 58 CCCTGTGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
203 CCTGGCGGTGG
Cac8I 453 GCACGC GC[ACGT]^[ACGT]GC
725 GCCTGC
EcoT14I 720 CCAAGG C^C[AT][AT]GG
753 CCAAGG
Fnu4HI 21 GCTGC GC^[ACGT]GC
319 GCTGC
ItaI 21 GCTGC GC^[ACGT]GC
319 GCTGC
StyI 720 CCAAGG C^C[AT][AT]GG
753 CCAAGG
TseI 21 GCTGC G^C[AT]GC
319 GCTGC
Tsp509I 152 AATT ^AATT
227 AATT
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# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AciI AluI AspHI AvaII BanII
BmyI BsaJI BsiHKAI BsmAI Bsp1286I
BsrI BssKI BstNI Cfr13I DdeI
DpnII DraII EcoO109I EcoRII HaeIII
HphI MaeIII MboI MboII MnlI
MslI MvaI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI Tsp45I TspRI