Restriction map for
pFN21AE1976
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# NO CUT ENZYMES ( 126 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BamHI BbeI
BbrPI BbvCI BcgI BciVI BfrI
BglII BlnI BsaBI BsaHI BseAI
BsiEI BsiWI BsmI BsoBI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FseI FspI Hin1I
HindIII HpaI KasI KpnI KspI
MamI MfeI MflI MluI MroI
MseI MunI MvnI NarI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SapI SbfI ScaI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspBI SspI
SwaI TaqI Tru9I Tth111I TthHB8I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AgeI 655 ACCGGT A^CCGGT
AlwI 715 GATCC GGATC(4/5)
AocI 917 CCTCAGG CC^T[ACGT]AGG
BanI 1271 GGCACC G^G[CT][GA]CC
BbsI 302 GAAGAC GAAGAC(2/6)
BclI 395 TGATCA T^GATCA
BlpI 261 GCTAAGC GC^T[ACGT]AGC
BpmI 460 CTGGAG CTGGAG(16/14)
Bpu1102I 261 GCTAAGC GC^T[ACGT]AGC
BpuAI 302 GAAGAC GAAGAC(2/6)
BsaAI 566 CACGTA [CT]AC^GT[GA]
BspHI 41 TCATGA T^CATGA
BsrBI 7 CCGCTC CCGCTC(-3/-3)
BsrDI 627 CATTGC GCAATG(2/0)
Bsu36I 917 CCTCAGG CC^T[ACGT]AGG
CelII 261 GCTAAGC GC^T[ACGT]AGC
EarI 178 GAAGAG CTCTTC(1/4)
Eco81I 917 CCTCAGG CC^T[ACGT]AGG
FbaI 395 TGATCA T^GATCA
HgaI 1389 GACGC GACGC(5/10)
HincII 1027 GTCAAC GT[CT]^[GA]AC
HindII 1027 GTCAAC GT[CT]^[GA]AC
HphI 468 GGTGA GGTGA(8/7)
Ksp632I 178 GAAGAG CTCTTC(1/4)
MslI 1422 CACCGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 509 GCCGGC GCC^GGC
NdeI 341 CATATG CA^TATG
NgoMIV 509 GCCGGC G^CCGGC
PinAI 655 ACCGGT A^CCGGT
PleI 24 GAGTC GAGTC(4/5)
RcaI 41 TCATGA T^CATGA
SexAI 1371 ACCAGGT A^CC[AT]GGT
SfiI 1104 GGCCCTTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 114 GCATGC GCATG^C
TfiI 471 GAATC G^A[AT]TC
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# TWO CUT ENZYMES ( 22 enzymes) #
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AcsI 1118 AAATTC [GA]^AATT[CT]
1399 GAATTT
ApoI 1118 AAATTC [GA]^AATT[CT]
1399 GAATTT
BcnI 779 CCCGG CC^[GC]GG
879 CCGGG
BglI 926 GCCTGTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1105 GCCCTTGGGGC
BsaWI 409 ACCGGA [AT]^CCGG[AT]
655 ACCGGT
BsmBI 12 CGTCTC CGTCTC(1/5)
224 GAGACG
BsrFI 509 GCCGGC [GA]^CCGG[CT]
655 ACCGGT
BssSI 53 CACGAG CACGAG(-5/-1)
503 CTCGTG
BstAPI 588 GCAGCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
851 GCAGCCAGTGC
Cfr10I 509 GCCGGC [GA]^CCGG[CT]
655 ACCGGT
DpnII 396 GATC ^GATC
715 GATC
DraIII 822 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
1370 CACCAGGTG
HaeII 608 GGCGCT [GA]GCGC^[CT]
839 AGCGCC
HinfI 24 GAGTC G^A[ACGT]TC
471 GAATC
MboI 396 GATC ^GATC
715 GATC
NciI 779 CCCGG CC^[GC]GG
879 CCGGG
NcoI 295 CCATGG C^CATGG
806 CCATGG
NdeII 396 GATC ^GATC
715 GATC
NspI 114 GCATGC [GA]CATG^[CT]
560 ACATGC
SacI 527 GAGCTC GAGCT^C
1071 GAGCTC
Sau3AI 396 GATC ^GATC
715 GATC
StuI 159 AGGCCT AGG^CCT
194 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AfaI AluI AlwNI ApaI
AspHI AvaII BalI BanII BbvI
BfaI BmyI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DraII DsaI EaeI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HpaII
ItaI MaeI MaeII MaeIII MboII
MluNI MnlI MscI MspA1I MspI
MvaI MwoI NlaIII NlaIV PflMI
PpuMI PstI PvuII RsaI Sau96I
ScrFI SfaNI SfcI StyI TseI
Tsp45I Tsp509I TspRI Van91I XcmI