Restriction map for
pFN21AB7277
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# NO CUT ENZYMES ( 65 enzymes) #
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AclI AcsI AgeI ApoI AscI
AseI AsnI Asp700I AspI AvrII
BbrPI BbsI BciVI BlnI BpuAI
BsaBI BsiWI BsmI BspDI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
DraI DrdI EcoO65I EcoRI EcoRV
EcoT22I FseI HincII HindII HindIII
HpaI MamI MfeI MluI MunI
NcoI NruI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I SalI ScaI SfuI
SgrAI SnaBI SpeI SphI SspI
SwaI Tsp509I Tth111I XbaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AatII 1792 GACGTC GACGT^C
Acc65I 1415 GGTACC G^GTACC
AccI 142 GTCTAC GT^[AC][GT]AC
AccIII 2952 TCCGGA T^CCGGA
AflII 2152 CTTAAG C^TTAAG
AflIII 1085 ACATGT A^C[GA][CT]GT
AhdI 2491 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2302 GTGCAC G^TGCAC
AocI 2842 CCTCAGG CC^T[ACGT]AGG
ApaLI 2302 GTGCAC G^TGCAC
Asp718I 1415 GGTACC G^GTACC
AspEI 2491 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 2525 TGGCCA TGG^CCA
BamHI 3838 GGATCC G^GATCC
BbvCI 3195 GCTGAGG CCTCAGC(-5/-2)
BclI 2333 TGATCA T^GATCA
BfrI 2152 CTTAAG C^TTAAG
BglII 2156 AGATCT A^GATCT
BseAI 2952 TCCGGA T^CCGGA
Bsp1407I 2591 TGTACA T^GTACA
BspEI 2952 TCCGGA T^CCGGA
BspHI 830 TCATGA T^CATGA
BspLU11I 1085 ACATGT A^CATGT
BsrDI 1206 CATTGC GCAATG(2/0)
BsrGI 2591 TGTACA T^GTACA
Bsu36I 2842 CCTCAGG CC^T[ACGT]AGG
CpoI 19 CGGTCCG CG^G[AT]CCG
Eam1105I 2491 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2842 CCTCAGG CC^T[ACGT]AGG
FbaI 2333 TGATCA T^GATCA
KpnI 1415 GGTACC GGTAC^C
MluNI 2525 TGGCCA TGG^CCA
MroI 2952 TCCGGA T^CCGGA
MscI 2525 TGGCCA TGG^CCA
MseI 2153 TTAA T^TAA
NheI 2134 GCTAGC G^CTAGC
NotI 255 GCGGCCGC GC^GGCCGC
PshAI 3609 GACCTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 1998 CGATCG CGAT^CG
RcaI 830 TCATGA T^CATGA
RsrII 19 CGGTCCG CG^G[AT]CCG
SexAI 1418 ACCTGGT A^CC[AT]GGT
SfiI 3003 GGCCATGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 2591 TGTACA T^GTACA
Tru9I 2153 TTAA T^TAA
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# TWO CUT ENZYMES ( 13 enzymes) #
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AviII 272 TGCGCA TGC^GCA
2015 TGCGCA
BcgI 2922 CGAGGAGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2937 CGAGGAGCCTGC
BsaAI 145 TACGTG [CT]AC^GT[GA]
1971 CACGTA
BssSI 2007 CACGAG CACGAG(-5/-1)
2548 CACGAG
DraIII 1493 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG
2296 CACATCGTG
EcoT14I 396 CCTTGG C^C[AT][AT]GG
3210 CCTTGG
FspI 272 TGCGCA TGC^GCA
2015 TGCGCA
NdeI 1410 CATATG CA^TATG
1722 CATATG
SacI 1240 GAGCTC GAGCT^C
2551 GAGCTC
SapI 3109 GAAGAGC GCTCTTC(1/4)
2083 GCTCTTC
SbfI 465 CCTGCAGG CCTGCA^GG
2265 CCTGCAGG
Sse8387I 465 CCTGCAGG CCTGCA^GG
2265 CCTGCAGG
StyI 396 CCTTGG C^C[AT][AT]GG
3210 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI Aor51HI ApaI AspHI
AvaI AvaII BanI BanII BbeI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssHII BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EarI EclXI Eco47III Eco52I
EcoNI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PaeR7I PflMI PleI PpuMI
PstI PvuII RsaI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI SmlI StuI TaqI TfiI
TseI Tsp45I TspRI TthHB8I Van91I
XcmI XhoI XhoII XmaCI XmaI